HU Tiantian,WANG Yali,YANG Xiulian,et al.Study on Pedicels and Leaves Callus Induction of Osmanthus fragrans[J].Northern Horticulture,2015,39(19):95-101.[doi:10.11937/bfyy.201519024]
桂花花梗与叶片的愈伤组织诱导研究
- Title:
- Study on Pedicels and Leaves Callus Induction of Osmanthus fragrans
- Keywords:
- tissue culture; callus; Osmanthus fragrans; mediums
- 文献标志码:
- A
- 摘要:
- 以桂花幼嫩花梗和叶片为外植体，通过植物组织培养技术，研究了桂花无菌体系的建立，不同品种群的桂花花梗在不同培养基配方下的出愈情况和植物生长调节剂、蔗糖浓度及暗处理时间等因素对不同桂花品种群的叶片愈伤组织诱导培养的影响。结果表明：桂花花梗最佳消毒方式为5% NaClO消毒3 min，叶片最佳消毒方式为0.1% HgCl2消毒5 min。花梗愈伤组织的诱导培养结果表明，“波叶金桂”的最佳配方为MS+2.00 mg/L 6-BA+0.40 mg/L 2,4-D；“紫梗籽银桂”为B5+2.00 mg/L 6-BA+0.40 mg/L 2,4-D；“雨城丹桂”为B5+1.00 mg/L 6-BA+0.10 mg/L 2,4-D；“日香桂”为B5+2.00 mg/L 6-BA+0.10 mg/L 2,4-D。叶片愈伤组织的诱导培养结果表明，最佳蔗糖浓度为25 g/L，最适宜的暗培养天数为22 d；最利于“雨城丹桂”叶片愈伤组织诱导培养基的配方为MS+3.00 mg/L 6-BA+1.00 mg/L 2,4-D，当6-BA浓度为2.00 mg/L为“天香台阁”的最佳诱导配方。
- Abstract:
- The young pedicles and leaves of Osmanthus fragrans were used as the explants in the study.Through the plant tissue culture techniques,the experiment studied the establishment of Osmanthus fragrans sterile system,pedicels callus induction in different media formulations of varieties of O.fragrans groups and the influences of plant growth regulators，sucrose concentration，dark processing time etc.to leaves callus induction of different varieties of O.fragrans groups. The results showed that the best way to sterilize pedicels was using 5% NaClO for 3 min,the best way to sterilize leaves was using 0.1% HgCl for 5 min.The best mediums for pedicels of different varieties of O.fragrans were as follow,O.fragrans ’Boye Jingui’ was MS+2.00 mg/L 6-BA+0.40 mg/L 2,4-D，O.fragrans ’Zigeng Zi Yingui’ was B5+2.00 mg/L 6-BA+0.40 mg/L 2,4-D，O.fragrans ‘Yucheng Dangui’ was B5+1.00 mg/L 6-BA+0.10 mg/L 2,4-D，O.fragrans ’Rixiang’ was B5+2.00 mg/L 6-BA+0.10 mg/L 2,4-D.The results of leaves callus induction showed that,the best sucrose concentration was 25 g/L；it was best to put the mediums in dark condition for 22 days.The best medium for leaves of O.fragrans ‘Yucheng Dangui’ was MS+3.00 mg/L 6-BA+1.00 mg/L 2,4-D.The best medium for O.fragrans ‘Tianxiang Taige’ was MS+2.00 mg/L 6-BA+0.50 mg/L 2,4-D.
参考文献/References:
[1]聂谷华，向其柏.桂花研究现状及其存在的问题[J].九江学院学报（哲学社会科学版），2008，26(3)：85-87. [2]张丽霞，王毅彰.桂花的离体快繁技术[J].江苏农业科学,2013(3):42-43. [3]蔡新玲,胡蕙露.桂花茎尖初代组织培养试验研究[J].安徽林业科技，2009 (1)：17-19. [4]胡海波,黄丹,许岳香.木犀科植物组织培养研究综述[J].林业科技开发,2009(3):5-8. [5]BONGA J M.树木组织培养[M].阙国宁,等译.北京:中国林业出版社，1988. [6]潘瑞炽.植物组织培养[M].广州:广东高等教育出版社，2000：20-21. [7]杜燕,蒋海玉,刘其宁.贮存过期油菜种子消毒方法的研究[J].种子,2003(2):39-40,42. [8]WANG Y P，LIU Q C，LI A X，et al．In vitro selection and identification of drought tolerant mutants in sweet potato[J].Agricultural Sciences in China，2003，2(12)：1314-1320. [9]董闪,李明,唐堃,等.白木香愈伤组织的诱导及培养[J].湖北农业科学,2014（15）:3673-3677. [10]和凤美,朱永平,杨晓红,等.冬樱花愈伤组织诱导和抑制褐化初探[J].中国农学通报，2010(12)：130-134. [11]宋会访,葛红,周媛,等.桂花离体培养与快速繁殖技术的初步研究[J].园艺学报,2005(4):738-740. [12]喻晓雁,刘克旺,梁文斌.穗花杉组织培养初探[J].中南林学院学报,2005(2):55-58. [13]任桂芳,王建红,冯慧,等.现代月季(Rosa hybrida)叶片植株再生体系的建立[J].园艺学报,2004(4):533-536. [14]蔡新玲.不同桂花品种群组织培养的研究[D].合肥：安徽农业大学,2007. [15]吕晋慧，孔冬梅.园艺植物组织培养[M].北京：中国农业科学技术出版社,2008:24. [16]彭尽晖,吕长平,周晨.四季桂愈伤组织诱导与继代培养[J].湖南农业大学学报(自然科学版)，2003(2)：131-133. [17]王文房,李修岭.樱花花柄的组织培养[J].安徽农业科学,2006(22):5839-5841. [18]王金刚,张兴.园林植物组织培养技术[M].北京：中国农业科学技术出版社，2008:27-28. [19]丁路明,龙瑞军,朱铁霞.2,4-D和6-BA对早熟禾愈伤组织诱导的影响[J].草原与草坪,2003(1):34-37. [20]周宜君,周生闯,刘玉,等.植物生长调节剂对植物愈伤组织的诱导与分化的影响[J].中央民族大学学报(自然科学版),2007（1）:23-28. [21]宋会访.桂花组织培养技术体系的研究[D].武汉：华中农业大学,2004.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(19):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(19):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(19):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(19):101.
[7]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(19):108.
[8]闫海霞,黄昌艳,何荆洲,等.观赏凤梨组织培养的影响因素分析[J].北方园艺,2013,37(22):196.
YAN Hai-xia,HUANG Chang-yan,HE Jing-zhou,et al.Analysis of the Influence Factors in Tissue Culture of Ornamental Bromeliads[J].Northern Horticulture,2013,37(19):196.
[9]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(19):89.
[10]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(19):91.
[11]高素燕,李素文,吕敬刚,等.影响辣椒愈伤组织诱导的关键因素分析[J].北方园艺,2014,38(07):99.
GAO Su-yan,LI Su-wen,LV Jing-gang,et al.Analysis of Key Factors on Influencing Callus Induction of Pepper[J].Northern Horticulture,2014,38(19):99.
[12]张华,李姝睿,龙丽坤.五味子种子萌发与愈伤组织的诱导[J].北方园艺,2013,37(03):164.
ZHANG Hua,LI Shu-rui,LONG Li-kun.Seed Germination and Callus Induction of Schisandra chinensis (Turcz) Bail[J].Northern Horticulture,2013,37(19):164.
[13]代月,吴雄,廉美兰,等.影响洋葱愈伤组织及胚性愈伤组织诱导的因素研究[J].北方园艺,2013,37(07):125.
DAI Yue,WU Xiong,LIAN Mei-lan,et al.Study on the Factors of Onion Callus and Embryonic Callus Induction[J].Northern Horticulture,2013,37(19):125.
[14]孟 倩,孔祥生,张改娜.不同植物生长调节剂对丹参组培快繁的影响[J].北方园艺,2013,37(09):102.
MENG Qian,KONG Xiang-sheng,ZHANG Gai-na.Effect of Different Growth Regulators on Tissue Culture and Rapid Propagation of Salvia miltiorrhiza [J].Northern Horticulture,2013,37(19):102.
[15]邵红,孙睿,张丽敏,等.北马兜铃组培快繁技术[J].北方园艺,2012,36(18):128.
SHAO Hong,SUN Rui,ZHANG Li-min,et al.Study on the Technique of Tissue Culture and Rapid Propagation of Aristolochia contorta Bunge[J].Northern Horticulture,2012,36(19):128.
[16]宋丽莉,司亮,于典司,等.亚洲百合“普瑞头”叶片组织培养技术研究[J].北方园艺,2012,36(06):122.
SONG Li-li,SI Liang,YU Dian-si,et al.Study on Tissue Culture and Micropropagation of Lilium asiatic Hybrids Prato[J].Northern Horticulture,2012,36(19):122.
[17]杨丽莉,张晓,杨睿,等.大花萱草‘莎蔓’的组织培养技术研究[J].北方园艺,2012,36(19):134.
YANG Li-li,ZHANG Xiao,YANG Rui,et al.Study on Tissue Cultivation Technique in the Hemerocallis hybrida ‘Shaman’[J].Northern Horticulture,2012,36(19):134.
[18]谭武平,梁毅,张洪伟,等.洋葱胚芽再生体系的研究[J].北方园艺,2014,38(16):93.
TAN Wu-ping,LIANG Yi,ZHANG Hong-wei,et al.Study on Embryo Regeneration System of Onion[J].Northern Horticulture,2014,38(19):93.
[19]李利霞,李婷婷,朱虹,等.濒危植物珠子参愈伤组织诱导因素分析[J].北方园艺,2016,40(07):85.[doi:10.11937/bfyy.201607022]
LI Lixia,LI Tingting,ZHU Hong,et al.Different Factors on Callus Induction of Panax japonicus var.major[J].Northern Horticulture,2016,40(19):85.[doi:10.11937/bfyy.201607022]
[20]都彦伶,赵新涛,宿烽.两种基因型黄瓜愈伤组织的诱导[J].北方园艺,2016,40(11):96.[doi:10.11937/bfyy.201611025]
DU Yanling,ZHAO Xintao,SU Feng.Induction of Two Genotypes Cucumber Callus[J].Northern Horticulture,2016,40(19):96.[doi:10.11937/bfyy.201611025]
备注/Memo
第一作者简介：胡甜甜（1991-），女，硕士，研究方向为园林植物遗传育种。E-mail:htt0726@163.com.责任作者：王良桂（1962-），男，博士，教授，博士生导师，研究方向为植物栽培与应用和园林工程管理。E-mail:wlg@njfu.com.cn.基金项目：“十二五”国家科技支撑计划资助项目（2013BA001B06）；江苏高校优势学科建设工程资助项目（PAPD）。