LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(22):108-110.
两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响
- Title:
- Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang
- 文章编号:
- 1001-0009(2013)22-0108-03
- Keywords:
- Citrus; gelling agent; callus; plant regeneration; Gelrite
- 分类号:
- S 666.2
- 文献标志码:
- A
- 摘要:
- 以冰糖橙胚性愈伤组织为试材，研究了琼脂及Gelrite 2种凝固剂对愈伤组织增殖、胚状体诱导、芽诱导及生根的影响。结果表明：以琼脂为凝固剂的培养基有利于提高胚性愈伤组织的增殖率，以Gelrite为凝固剂的培养基有利于胚状体诱导、芽诱导和生根。因此，在愈伤组织增殖过程中可使用琼脂，在体细胞胚状体诱导、芽诱导和生根阶段可使用Gelrite。
- Abstract:
- Taking embryogenic callus of Citrus sinensis L.Osbeck cv.Bingtang as initial explants,the effects of two types of gelling agents,agar and gelrite on the callus proliferation,somatic embryoid induction,bud induction and rooting in vitro was studied.The results showed that the culture medium prepared with agar was helpful for improving the proliferation rate of the embryogenic callus,and the culture medium prepared with gelrite facilitated somatic embryoid induction,bud induction and rooting of the embryogenic callus.Therefore,agar may be used during the process of callus culture,and it may be more reasonable of using gelrite as a gelling agent at the stages of somatic embryogenesis,bud induction and rooting.
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备注/Memo
第一作者简介：李松丽（1979-），女，河南南阳人，硕士，实验师，现主要从事园艺植物逆境生理研究工作。
责任作者：蔡小东（1978-），男，湖北浠水人，博士，副教授，现主要从事园艺植物生物技术育种研究工作。E-mail:caixiao.dong@163.com.
基金项目：经济林木种质改良与资源综合利用湖北省重点实验室开放课题资助项目（20011BLKF239）。
收稿日期：2013-06-19