MENG Qian,KONG Xiang-sheng,ZHANG Gai-na.Effect of Different Growth Regulators on Tissue Culture and Rapid Propagation of Salvia miltiorrhiza [J].Northern Horticulture,2013,37(09):102-105.
不同植物生长调节剂对丹参组培快繁的影响
- Title:
- Effect of Different Growth Regulators on Tissue Culture and Rapid Propagation of Salvia miltiorrhiza
- 文章编号:
- 1001-0009(2013)09-0102-04
- 分类号:
- S 567.5
- 文献标志码:
- A
- 摘要:
- 以丹参无菌苗叶片为外植体，以MS为基本培养基，研究了不同种类和浓度的植物生长调节剂对丹参叶片愈伤组织诱导、芽诱导及根诱导的影响。结果表明：MS+0.5 mg/L 2,4-D+1.0 mg/L 6-BA利于丹参叶片诱导愈伤；诱导愈伤分化为芽的最适培养基为MS+0.5 mg/L NAA+1.5 mg/L 6-BA；诱导生根的最适培养基为1/2MS+0.5 mg/L NAA。该试验为丹参的工厂化生产奠定了理论基础。
- Abstract:
- Taking the leaves of aseptic seedling as explants，using MS as basic medium，the effect of different kinds and concentrations of plant growth regulators on tissue culture of blade callus，bud and root induction of Salvia miltiorrhiza were studied.The results showed that MS+0.5 mg/L 2,4-D+1.0 mg/L 6-BA culture medium was suitable for blade of Salvia miltiorrhiza induce callus.MS+0.5 mg/L NAA+1.5 mg/L 6-BA medium was the optimum medium for callus differentiate bud.1/2MS+0.5 mg/L NAA was the optimum medum for root induction.It provided a theoretical basis for its industrial production.
参考文献/References:
[1]肖培根.新编中药志[S].北京:化学工业出版社,2001:212. [2]中国药典[S].卷I,2005. [3]Liu J，Shen H M，Narnong C.Salvia miltiorrhizainhibits cell growth and induces apoptosis in human hepatoma HepG2 cells[J].Cancer Lett,2000,153:85-93. [4]单成钢,王志芬,苏学合,等.丹参组织培养研究进展[J].现代中药研究与实践,2007,33(2):54-57. [5]赵东利,曹雪梅,邱桂佳,等.丹参的组织培养及植株再生[J].北京农学院学报,2010,25(2):5-7. [6]柳福智,董娟娥,梁宗锁.不同生长调节物质对丹参愈伤组织的诱导效应[J].中国农学通报,2005,21(11):202-204,275. [7]杨精华,刘曦,张乐,等.崖白菜的愈伤组织诱导及植株再生[J].植物生理学报,2012,48(7):664-668. [8]全妙华,欧立军,贺安娜,等.天门冬组培快繁体系研究[J].中草药,2012,8(43):1599-1603. [9]陈书安,王晓东,欧阳杰,等.藏红花球茎愈伤组织快速诱导的研究[J].中国药学杂志,2003,38(4):254-256. [10]梁宏伟,王锋祥,崔秀伟.白花丹参的组织培养和植株再生[J].安徽农业科学,2009,37(21):9876,9904.
相似文献/References:
[1]王海新,赵艳,尤淑丽,等.植物生长调节剂“易丰收”对树莓组培苗生根的影响[J].北方园艺,2013,37(04):31.
[2]陶米林,刘清波,黄红梅,等.半夏组织培养体系的建立[J].北方园艺,2013,37(04):113.
[3]曹 虹,金荣荣.三倍体薄皮甜瓜未成熟胚子叶诱导不定芽的研究[J].北方园艺,2014,38(13):106.
CAO Hong,JIN Rong-rong.Study on Adventitious Bud From Immature Embryo Cotyledon of Triploid Muskmelon[J].Northern Horticulture,2014,38(09):106.
[4]张振霞,李彩萍,张秋枚,等.甘肃贝母的组织培养和植株再生研究[J].北方园艺,2014,38(10):80.
ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(09):80.
[5]王玉娇,杨 忠,赵春莉,等.兴安白头翁组织培养与快繁技术研究[J].北方园艺,2014,38(10):84.
WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(09):84.
[6]朱 军,李晓瑾,孙 丽,等.药用植物玛咖离体快繁技术研究[J].北方园艺,2013,37(22):101.
ZHU Jun,LI Xiao-jin,SUN Li,et al.Study on the Rapid Propagation of Medicinal Plants Lepidium meyenii[J].Northern Horticulture,2013,37(09):101.
[7]杜 丽.香樟子叶胚培养的研究[J].北方园艺,2014,38(14):89.
DU Li.Research on Cotyledonary Embryo Culture of Cinnamomum camphora L.[J].Northern Horticulture,2014,38(09):89.
[8]赵丽莉,徐芳芳,荀 洋,等.桔梗花药愈伤组织诱导培养基筛选[J].北方园艺,2014,38(01):95.
ZHAO Li-li,XU Fang-fang,XUN Yang,et al.Screening of Callus Induction Medium of Platycodon grandiflorumAnther[J].Northern Horticulture,2014,38(09):95.
[9]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(09):106.
[10]陈丽文,杨利平,荣薏,等.金心也门铁工厂化育苗技术研究[J].北方园艺,2013,37(13):138.
CHEN Liwen,YANG Li-ping,RONG Yi,et al.Study on the Techniques of Industrialized Seedling Culture of Draceana arborea[J].Northern Horticulture,2013,37(09):138.
[11]房师梅,雷世俊,王洪波,等.丹参组培快繁技术研究[J].北方园艺,2013,37(21):123.
FANG Shi-mei,LEI Shi-jun,WANG Hong-bo,et al.Study on Tissue Culture and Rapid Propagation of Salvia[J].Northern Horticulture,2013,37(09):123.
备注/Memo
第一作者简介：孟倩（1987-），女，河南郑州人，硕士，研究方向为植物学。E-mail:damengqian@163.com.