WANG Yu-jiao,YANG Zhong,ZHAO Chun-li,et al.Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng[J].Northern Horticulture,2014,38(10):84-87.
兴安白头翁组织培养与快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation of Pulsatill dahurica（Fisch.）Spreng
- 文章编号:
- 1001-0009（2014）10-0084-04
- 分类号:
- S 567
- 文献标志码:
- A
- 摘要:
- 以兴安白头翁种子无菌苗幼芽为外植体，进行了组织培养与快繁技术研究。结果表明：兴安白头翁种子以0.1%氯化汞灭菌的最佳时间为4 min；种子萌发的最佳培养基为MS+IAA 0.1 mg/L；兴安白头翁愈伤组织最佳诱导培养基为MS+2,4-D 0.15 mg/L+6-BA 1.0 mg/L；培养基MS+IAA 1.0 mg/L+ZT 0.2 mg/L最适合兴安白头翁愈伤组织不定芽的诱导；1/2MS+NAA 2.0 mg/L为兴安白头翁的最佳生根培养基；草炭、园土及其等比混合基质对兴安白头翁移栽成活率影响不大，均达90%以上。
- Abstract:
- Using aseptic shoots from seed of Pulsatill dahurica（Fisch.）Spreng as explants，tissue culture and rapid propagation of Pulsatill dahurica（Fisch.）Spreng were established and studied.The results showed that the seed of Pulsatill dahurica（Fisch.）Spreng optimum sterilization time was 4 min by 0.1% HgCl2；the best combination of culture medium was MS+IAA 0.1 mg/L；optimum induced medium was MS+2,4-D 0.15 mg/L+6-BA 1.0 mg/L；optimal proliferation medium was MS+IAA 1.0 mg/L+ZT 0.2 mg/L；the rooting medium was 1/2MS+NAA 2.0 mg/L,the growth was the best;different medium (grass ashes,garden soil,and its equal ratio) had little influence on survival rate of transplanting,all above 90%.
参考文献/References:
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备注/Memo
第一作者简介：王玉娇（1987-），女，山东临沂人，硕士研究生，研究方向为园林植物资源与种质创新。E-mail：shuyi79@163.com.