WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(01):91-94.
长筒石蒜叶片薄层培养诱导愈伤组织发生的研究
- Title:
- Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture
- 文章编号:
- 1001-0009(2014)01-0091-04
- Keywords:
- Lycoris longituba; leaves; thin cell layer; callus
- 分类号:
- Q 949.71+8.25
- 文献标志码:
- A
- 摘要:
- 以切成2 mm左右的长筒石蒜幼嫩叶片薄片作为外植体，研究了不同激素组合以及不同接种方式对其愈伤组织诱导的影响，以期获得长筒石蒜叶片愈伤诱导的最佳条件。结果表明：在1/2MS+NAA 0.5 mg/L+TDZ 2.0 mg/L+蔗糖30 g/L+土豆粉3 g/L培养基中，愈伤诱导率最高可达90.0%，TDZ诱导愈伤效果明显优于2,4-D；采用叶片垂直插入培养基的接种方式，培养30 d后获得愈伤诱导率最高为83.3%。
- Abstract:
- The young leaves of Lycoris longituba that were cut into 2 mm slices as explants,which cultured in vitro on several mediums supplemented with diverse concentrations of plant growth regulator and different inoculation method in order to find the best conditions of callus induction.The results showed that the highest induction rate 90.0% was obtained from the mediums which consist of 1/2MS+NAA 0.5 mg/L+TDZ 2.0 mg/L+ sucrose 30 g/L+dried potato powder 3 g/L.Callus induction effect of TDZ was obviously better than that of 2,4-D；adopts blades vertically inserted into the culture medium of inoculation method,cultivation of 30 days could obtain the highest callus induction rate of 83.3%.
参考文献/References:
[1] 张海珍,周兔英,鲍淳松,等.“中国的郁金香”-石蒜属快繁及园林应用研究[J].园林科技,2012(1):5-7. [2] 王磊,汤庚国,刘彤.长筒石蒜切花衰败生理及超微结构的变化[J].浙江林学院学报,2009(4):498-502. [3] 谷海燕,谢孔平,李世丽,等.石蒜属植物的无性繁殖研究[J].中国林副特产,2012(2):32-35. [4] 时剑,童再康,刘志高,等.换锦花种胚和叶片的组织培养研究[J].江西农业大学学报，2011(4):665-669. [5] Steinmacher D A,Krohn N G,Dantas A C M,et al.Somatic embryogenesis in peach palm using the thin cell layer technique：induction,morpho-histological aspects and AFLP analysis of somaclonal variation[J].Annals of Botany,2007,100:699-709. [6] 赵志敏,钟湘云,杨帅,等.黄花石蒜愈伤组织培养研究[J].湖南中医药大学学报,2009(5):48-50. [7] 刘合霞,周坚.石蒜愈伤组织的诱导及其植株再生研究[J].北方园艺,2013(6):93-96. [8] Lee Y,Lee D E,Lee H S,et al.Influence of auxins,cytokinins,and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree (Morus albaL.)[J].Plant Cell Tissue Organ Culture,2011,105:9-19. [9] Guedes R S,Silva T L,Luis Z G,et al.Initial requirements for embryogenic calluses initiation in thin cell layers explants from immature female oil palm inflorescences[J].African Journal of Biotechnology,2011,10(52):10774-10780. [10]王关林,方宏筠,那杰.高活性细胞激动素TDZ在植物组织培养中的应用[J].植物学通报,1997(3):48-54. [11]周红菊.TDZ对欧亚旋覆花愈伤组织诱导的影响研究[J].湖北农业科学,2010(4):798-799. [12]Nhut D T,An T T T,Nguyen H T D,et al.Effect of genotype,explant size,position,and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture[J].Scientia Horticulturae,2007,111:146-151. [13]王跃华,张阔军,张丽君,等.川贝母叶高效诱导愈伤组织体系的研究[J].安徽农业科学,2011(3):1374-1375.
相似文献/References:
[1]张玉杰,杨 忠,顾德峰.狗枣猕猴桃叶片离体培养的研究[J].北方园艺,2014,38(10):97.
ZHANG Yu-jie,YANG Zhong,GU De-feng.Study on in vitro Culture of Actinidia kolomikta Leaves[J].Northern Horticulture,2014,38(01):97.
[2]鲍淳松,张鹏翀,周 虹,等.切根种植对长筒石蒜生长的影响[J].北方园艺,2013,37(22):92.
BAO Chun-song,ZHANG Peng-chong,ZHOU Hong,et al.Effects of Root-cutting on Growth of Lycoris longituba[J].Northern Horticulture,2013,37(01):92.
[3]杜社妮,耿桂俊,张 蕊,等.向日葵光合作用能力最强叶片探讨及其光合作用日变化研究[J].北方园艺,2014,38(01):11.
DU She-ni,GENG Gui-jun,ZHANG Rui,et al.Exploration and Research on the Strongest Photosynthetic Capacity and Diurnal Photosynthetic Dynamics Leaf of Sunflower[J].Northern Horticulture,2014,38(01):11.
[4]常立儒.‘山梨’果实和叶片发育期有机酸含量的变化及其相关性研究[J].北方园艺,2014,38(04):26.
CHANG Li-ru.Correlation Analysis of Changes in the Organic Acid Contents of Sorb During Fruit and Leaf Development[J].Northern Horticulture,2014,38(01):26.
[5]卢伟红,张玉星.鸭梨幼龄和成树叶片中营养元素周年变化规律研究[J].北方园艺,2013,37(14):40.
LU Wei-hong,ZHANG Yu-xing.Study on Annual Change Rule of Nutritive Elements in Young and Mature Leaves of Yali Pears[J].Northern Horticulture,2013,37(01):40.
[6]蒋燕,王进涛,袁红.激素配比对辣椒叶片愈伤组织诱导和不定芽分化的影响[J].北方园艺,2013,37(14):118.
JIANG Yan,WANG Jin-tao,YUAN Hong.Effect of Hormone Combinations on Callus Induction and Adventitious Bud Differentiation of Pepper Leaves[J].Northern Horticulture,2013,37(01):118.
[7]姜喜,马宝蕴,陈加利,等.香梨生长季果实和叶片矿质元素含量的变化[J].北方园艺,2013,37(12):15.
JIANG Xi,MA Bao-yun,CHEN Jia-li,et al.Variation of Mineral Element Content in Fragrant Pear Fruits and Leaves During Its Development[J].Northern Horticulture,2013,37(01):15.
[8]邢小明,林夏珍,王旭艳,等.波叶红果树叶片愈伤组织诱导研究[J].北方园艺,2013,37(12):108.
XING Xiao-ming,LIN Xia-zhen,WANG Xu-yan,et al.Study on Callus Inducement of Leaf Explant from Stranvaesia davidiana var.undulate[J].Northern Horticulture,2013,37(01):108.
[9]孙海燕,郭伟.正交实验优选向日葵叶片提取总黄酮的研究[J].北方园艺,2013,37(03):16.
SUN Hai-yan,GUO Wei.Study on the Total Flavonoids Extrated from the Leaves of Sunflower by Orthogonal Test[J].Northern Horticulture,2013,37(01):16.
[10]郑国琴.,柳金凤.宁夏枸杞叶片组培快繁技术研究[J].北方园艺,2012,36(01):122.
ZHENG Guo-qin.,LIU Jin-feng.Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.[J].Northern Horticulture,2012,36(01):122.
备注/Memo
第一作者简介：王婷（1988-），女，硕士研究生，现主要从事石蒜生长发育及繁殖等研究工作。