ZHANG Hua,LI Shu-rui,LONG Li-kun.Seed Germination and Callus Induction of Schisandra chinensis (Turcz) Bail[J].Northern Horticulture,2013,37(03):164-167.
五味子种子萌发与愈伤组织的诱导
- Title:
- Seed Germination and Callus Induction of Schisandra chinensis (Turcz) Bail
- 文章编号:
- 1001-0009(2013)03-0164-04
- Keywords:
- Schisandra chinensis (Turcz) Bail; tissue culture; explant; callus
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 以五味子种子为外植体，研究了不同种类培养基、不同植物生长调节剂、蔗糖及水解酪蛋白对五味子种子萌发和愈伤组织诱导的影响。结果表明：以GA 0.1 mg/L、蔗糖20 g/L、琼脂5.5 g/L为基本培养基时，五味子的种子快速大量萌发，此时，五味子的胚轴诱导愈伤组织出愈率最高为87%，完成愈伤组织的诱导需35 d左右。
- Abstract:
- The effect of culture medium，plant growth regulator，sucrose and casein hydrolysate on the seeds germination and callus induction of Schisandra were studied.The results showed that the seeds of Schisandra had a high frequency regeneration when taking GA 0.1 mg/L，sucrose 20 g/L，agar 5.5 g/L as the basic medium.Callus induction of Schisandra hypocotyl had the highest rate (87%) than that of other explants.Completion of callus induction took about 35 days.
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备注/Memo
第一作者简介：张华（1983-），女，硕士，助理研究员，现主要从事植物组织培养技术研究工作。