ZHANG Zhen-xia,LI Cai-ping,ZHANG Qiu-mei,et al.Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim[J].Northern Horticulture,2014,38(10):80-84.
甘肃贝母的组织培养和植株再生研究
- Title:
- Study on Tissue Culture and Plant Regeneration of Fritillaria przewalskii Maxim
- 文章编号:
- 1001-0009（2014）10-0080-05
- Keywords:
- Fritillaria przewalskii Maxim; tissue culture; bulb; proliferation
- 分类号:
- S 567.23+.1
- 文献标志码:
- A
- 摘要:
- 以甘肃贝母地下鳞茎为试材，研究了不同消毒方法、不同激素组合对其鳞茎的诱导增殖及植株再生的影响，以期建立甘肃贝母的快速无性繁殖体系。结果表明：用70%乙醇处理15 s，0.1%升汞处理 10 min，无菌水洗5次，然后在4℃中冷藏过夜，第2天重复上述灭菌步骤的连续消毒的方式灭菌效果很好，其染菌率为16.7%；诱导鳞茎不定芽的最佳培养基是MS+NAA 2.0 mg/L+6-BA 0.5 mg/L，出芽率较高，为87.5%，能分化出的绿色健壮的不定芽；继代增殖培养基是MS+NAA 0.5 mg/L+6-BA 2.0 mg/L，增殖系数达到9；生根的最佳培养基是1/2MS+NAA 0.5 mg/L，生根率90%。该试验基本建立了甘肃贝母植株的再生体系，为其愈伤组织的增殖和完整植株的再生提供了重要的研究基础。
- Abstract:
- In order to establish a rapid reproduction system of Fritillaria przewalskii Maxim，using bulb of Fritillaria przewalskii as material,the sterilization methods were optimized，combination of different plant hormones on the induced proliferation of bulbs from F.przewalskii were studied.The results showed that the procedure of optimal sterilization method were that，the materials should be immersed in 70% alcohol for 15 s，immerged in 0.1% mercuric chloride for 10 min，rinsed with sterile water 5 times，place in 4℃ for overnight；besides，the procedure should be repeated on the second day.Following above-mentioned steps，contamination rate of the materials was only 16.7%.The optimal culture medium that could induce bulbs of F.przewalskii to adventitious buds was MS+NAA 2.0 mg/L+6-BA 0.5 mg/L,and the induction rate had reached to 87.5%.The best subculture medium was MS+NAA 0.5 mg/L+6-BA 2.0 mg/L，and the multiplication coefficient was 9.The best rooting medium was 1/2MS+NAA 0.5 mg/L，and the rooting rate was about 90%.The paper had established regeneration system of F.przewalskii，which could provide the basic research reference for high-performance seedling methods of F.przewalskii.
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备注/Memo
第一作者简介：张振霞（1975-），女，博士，副教授，现主要从事植物生物技术等研究工作。E-mail：zhangzhenxia2006@gmail.com.