DAI Yue,WU Xiong,LIAN Mei-lan,et al.Study on the Factors of Onion Callus and Embryonic Callus Induction[J].Northern Horticulture,2013,37(07):125-127.
影响洋葱愈伤组织及胚性愈伤组织诱导的因素研究
- Title:
- Study on the Factors of Onion Callus and Embryonic Callus Induction
- 文章编号:
- 1001-0009(2013)07-0125-03
- Keywords:
- onion (Allium cepa L.); callus; embryogenic callus; tissue culture
- 分类号:
- S 633.203.6
- 文献标志码:
- A
- 摘要:
- 以洋葱雄性不育系种子（10-8F）为外植体，研究了蔗糖浓度、暗处理及取材时间对洋葱愈伤组织及胚性愈伤组织诱导的影响，以期优化胚性愈伤组织的形成条件，为转基因提供良好受体并为建立高频再生体系奠定基础。结果表明：40 g/L蔗糖及光培养最适合愈伤组织的诱导；50 g/L蔗糖及暗培养最适合胚性愈伤组织的诱导，最适宜的取材时间为45 d。
- Abstract:
- Taking the male sterility line seeds of onion (Allium cepa L.) as explants，the effects of sucrose concentration，light and sampling time on callus tissues and embryogenic callus tissues induction were studied，in order to optimize the conditions of embryogenic callus formation，and provide a good receptor transgenic and lay the foundation for the establishment of high frequency regeneration system.The results showed that 40 g/L sucrose and light culture were the most suitable for callus induction；50 g/L sucrose and dark culture were the most suitable for embryogenic callus induction，and the optimum sampling time was the 45th day.
参考文献/References:
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备注/Memo
第一作者简介：代月（1987-），女，在读硕士，研究方向为植物组织培养。E-mail:daiyue1030@126.com.