LI Qingwei,HOU Dianming,SHEN Shunxian.Establishment of Leaf Regeneration System of Crassula arborescens[J].Northern Horticulture,2016,40(17):107-110.[doi:10.11937/bfyy.201617025]
燕子掌叶片再生体系的建立
- Title:
- Establishment of Leaf Regeneration System of Crassula arborescens
- Keywords:
- Crassula arborescens; leaf; adventitious buds; regeneration
- 文献标志码:
- A
- 摘要:
- 以燕子掌叶片为试材，采用植物组织培养方法，研究了消毒时间、不同浓度2,4-D、6-BA、NAA对燕子掌再生的影响。结果表明:叶片用70%～75%的酒精浸泡10～15 s，再用0.1% HgCl2消毒12～15 min，具有较低污染率和较高的存活率;用MS+1.5 mg?L-1 6-BA+0.5 mg?L-1 2,4-D进行不定芽诱导，平均可以产生13.7个不定芽；不定芽在MS+6-BA 1.5 mg?L-1+NAA 0.1 mg?L-1+GA3 0.5 mg?L-1中增殖倍数为11.0；在1/2MS+IBA 0.5 mg?L-1或MS+IBA 0.5 mg?L-1 培养基中平均能产生9～11条根。
- Abstract:
- Taking Crassula arborescens leaves as materials，using plant tissue culture method，the effects of sterilizing time and different concentration of 2,4-D，6-BA，NAA on regeneration of Crassula arborescens were studied.The results showed that soaking the leaf in 70%-75% alcohol for 10-15 seconds，then 0.1% HgCl2 disinfection 12-15 minutes，the callus had low contamination rate and high survival rate.MS+1.5 mg?L-1 6-BA+0.5 mg?L-1 2,4-D was optimum medium for inducing adventitious buds with an average of 13.7 adventitious buds；adventitious buds in MS+6-BA 1.5 mg?L-1+NAA 0.1 mg?L-1+GA3 0.5 mg?L-1，the proliferation coefficient was 11.0；multiple shoots could produce an average of 9-11 roots in the 1/2MS+IBA 0.5 mg?L-1 or MS+IBA 0.5 mg?L-1 medium.
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备注/Memo
第一作者简介：李庆伟（1979-)，男，河南封丘人，硕士，讲师，研究方向为种苗繁育与大蒜生产。E-mail：liqingwei2003@163.com.