JIANG Yan,WANG Jin-tao,YUAN Hong.Effect of Hormone Combinations on Callus Induction and Adventitious Bud Differentiation of Pepper Leaves[J].Northern Horticulture,2013,37(14):118-120.
激素配比对辣椒叶片愈伤组织诱导和不定芽分化的影响
- Title:
- Effect of Hormone Combinations on Callus Induction and Adventitious Bud Differentiation of Pepper Leaves
- 文章编号:
- 1001-0009(2013)14-0118-03
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 以雄性不育系辣椒“071”的第9~11节叶片为外植体，研究了不同浓度激素配比对辣椒叶片愈伤组织诱导和不定芽分化的影响。结果表明：6-BA与NAA配合使用对愈伤组织的诱导效果较好，但不能促进不定芽的发生，其中6-BA 3.0 mg/L+NAA 0.1 mg/L诱导愈伤组织速度快，诱导率为100%，愈伤组织良好。NAA浓度在0.05、0.1 mg/L，6-BA浓度在0.2~5.0 mg/L之间时，6BA的浓度越高，愈伤组织越易发生褐化现象；6-BA和IAA配合使用，愈伤组织的分化率达50%左右，并促进不定芽的发生，其中6BA 50 mg/L+IAA 0.5 mg/L配比时，叶片不定芽生成诱导率较高为35.29％。N苯基N′-1,2,噻二唑5脲（TDZ）浓度为0.05、0.1 mg/L时，单独使用或与NAA、IAA配合使用，都无不定芽出现。
- Abstract:
- Taking the section 9~11 blade male sterile pepper ‘071’ as explants,the effects of different concentrations of hormone combinations on callus induction and adventitious bud differentiation of pepper leaves were studied.The results showed that the callus induction effect of the use of 6-BA and NAA was good,but could not promote adventitious bud differentiation occurred.On the medium of 6-BA 3.0 mg/L+NAA 0.1 mg/L,the callus had the fastest induction speed,with callus induction rate 100%.The concentration of NAA in the 0.05,0.1 mg/L,6-BA concentration in 0.2~5.0 mg/L,the higher the concentration of 6-BA,the callus was prone to browning；and with the use of 6-BA and IAA,the callus differentiation rate was about 50%,and could promote the occurrence of adventitious bud differentiation.With 6-BA 5.0 mg/L+IAA 0.5 mg/L ratio,bud induction rate of leaf blade was higher for 35.29%.The concentration of TDZ was 0.05,0.1 mg/L,used alone or in conjunction with the NAA,IAA,all without exception bud emergence.
参考文献/References:
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备注/Memo
第一作者简介：蒋燕（1966-），女，河南洛阳人，硕士，副教授，现主要从事园艺专业教学与科研工作。