ZHANG Yu-jie,YANG Zhong,GU De-feng.Study on in vitro Culture of Actinidia kolomikta Leaves[J].Northern Horticulture,2014,38(10):97-101.
狗枣猕猴桃叶片离体培养的研究
- Title:
- Study on in vitro Culture of Actinidia kolomikta Leaves
- 文章编号:
- 1001-0009（2014）10-0097-05
- Keywords:
- Actinidia kolomikta; leave; culture in vitro
- 分类号:
- S 663.4
- 文献标志码:
- A
- 摘要:
- 以狗枣猕猴桃叶片为外植体，研究了影响狗枣猕猴桃愈伤组织诱导、分化、试管苗生根的主要因素。结果表明：狗枣猕猴桃叶片诱导愈伤组织的最佳培养基为MS+ZT 1.0 mg/L+NAA 0.2 mg/L，诱导率达到91.67%；不定芽分化的最佳培养基为MS+ZT 2.0 mg/L+IBA 0.2 mg/L，分化率为89.45%，平均出芽个数为8.11个；狗枣猕猴桃极易生根，在以添加不同质量浓度的IAA、IBA和NAA的 1/2MS基本培养基上，生根率都很高，多数处理可达100%；当基质配比V(田园土)∶V(草炭)∶V(沙子)=1∶1∶1时，试管苗的成活率为92%。
- Abstract:
- Effect of different combinations of plant growth regulators on callus induction,differentiation and rooting of the test tube seedlings were studied by using leaves of Actinidia kolomikta as explants.The results showed that the optimal medium for callus inducement from leaf was MS+ZT 1.0 mg/L+NAA 0.2 mg/L，and callus inducement rate reached 91.67 percent；the best medium for differentiation of adventitious buds was MS+ZT 2.0 mg/L+IBA 0.2 mg/L,and the differentiation rate was 89.45 percent with an average number of 8.11 budding.Actinidia kolomikta was extremely easy to rooting,and to add different concentrations of IAA，IBA and NAA on 1/2MS medium，rooting rate were very high，and most even achieved 100 percent；as matrix consisted of garden soil，peat and sand by a volume ratio of 1∶1∶1 cultures the test tube seedlings，the survival rate was 92 percent.
参考文献/References:
[1]朱有昌．东北药用植物[M]．哈尔滨：黑龙江科学技术出版社，1989:740-741．
[2]张继有，严仲铠，长白山植物药志[M]．长春：吉林人民出版社，1982:983．
[3]李平亚，赵春芳，刘金平．猕猴桃的化学成分和生物活性研究进展[M].长春：吉林科学技术出版社，2006.
[4]李结维，毛世忠，梁木源，等，猕猴桃属植物果实营养成分的研究[J]．广西植物志，1995，15(4)：377-382．
[5]常晓丽，马冰如，何玲，狗枣猕猴桃叶化学成分的研究(I)[J]．中草药，1993 ,24(6):283-285.
[6]常晓丽，陈仑，马冰如．狗枣猕猴桃化学成分研究[J].中草药，1996，27(7)：395．
[7]赵继红，梁宇，颜达予，黄酮类化合物抗氧化活性的结构因素[J]．北方工业大学学报，2001，13 (1)：36-42．
[8]吴怀恩，甄汉深，钟振国．猕猴桃属植物的研究进展[J]．中药材，2004,27(1)：59-63．
[9]欧阳红涛，华卡，董建辉，猕猴桃药理作用的研究进展[J].中国医药导报，2007，32(4)：12-13．
[10] Hirsch A M The culture of Actinidia chinensis fruit tissues and themetabolism of free amino acids in Stem segments cultured锄vitro [J].Comptes Rendus Hebdomadaires des Seances de l’ Academic des Sciences D,1975,280(11):1369-1372.
[11]桂耀林，猕猴桃离体茎段愈伤组织的诱导和植株再生[J].植物生理学通讯，1979，21(4)：339-343．
[12]朱俊义，夏广清，刘雪莲，等．东北刺人参组培快繁及种质保存技术[J].东北林业大学学报，2007，35(11)：9-10.
[13]刘金英，徐有明，李双来，等．佛手山药组织培养的研究[J].植物研究，2006,26(3):323-237.
[14]李瑞芬，张敬原，赵茂林．结缕草愈伤组织诱导及植株再生[J]．园艺学报，2003，30(3)：355-357．
[15]张喜春，吴绛云．狗枣称猴桃叶片植株再生及无性系快速繁殖[J]．北方园艺，1990(9)：7-9．
相似文献/References:
[1]杜社妮,耿桂俊,张 蕊,等.向日葵光合作用能力最强叶片探讨及其光合作用日变化研究[J].北方园艺,2014,38(01):11.
DU She-ni,GENG Gui-jun,ZHANG Rui,et al.Exploration and Research on the Strongest Photosynthetic Capacity and Diurnal Photosynthetic Dynamics Leaf of Sunflower[J].Northern Horticulture,2014,38(10):11.
[2]常立儒.‘山梨’果实和叶片发育期有机酸含量的变化及其相关性研究[J].北方园艺,2014,38(04):26.
CHANG Li-ru.Correlation Analysis of Changes in the Organic Acid Contents of Sorb During Fruit and Leaf Development[J].Northern Horticulture,2014,38(10):26.
[3]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(10):91.
[4]卢伟红,张玉星.鸭梨幼龄和成树叶片中营养元素周年变化规律研究[J].北方园艺,2013,37(14):40.
LU Wei-hong,ZHANG Yu-xing.Study on Annual Change Rule of Nutritive Elements in Young and Mature Leaves of Yali Pears[J].Northern Horticulture,2013,37(10):40.
[5]蒋燕,王进涛,袁红.激素配比对辣椒叶片愈伤组织诱导和不定芽分化的影响[J].北方园艺,2013,37(14):118.
JIANG Yan,WANG Jin-tao,YUAN Hong.Effect of Hormone Combinations on Callus Induction and Adventitious Bud Differentiation of Pepper Leaves[J].Northern Horticulture,2013,37(10):118.
[6]姜喜,马宝蕴,陈加利,等.香梨生长季果实和叶片矿质元素含量的变化[J].北方园艺,2013,37(12):15.
JIANG Xi,MA Bao-yun,CHEN Jia-li,et al.Variation of Mineral Element Content in Fragrant Pear Fruits and Leaves During Its Development[J].Northern Horticulture,2013,37(10):15.
[7]邢小明,林夏珍,王旭艳,等.波叶红果树叶片愈伤组织诱导研究[J].北方园艺,2013,37(12):108.
XING Xiao-ming,LIN Xia-zhen,WANG Xu-yan,et al.Study on Callus Inducement of Leaf Explant from Stranvaesia davidiana var.undulate[J].Northern Horticulture,2013,37(10):108.
[8]孙海燕,郭伟.正交实验优选向日葵叶片提取总黄酮的研究[J].北方园艺,2013,37(03):16.
SUN Hai-yan,GUO Wei.Study on the Total Flavonoids Extrated from the Leaves of Sunflower by Orthogonal Test[J].Northern Horticulture,2013,37(10):16.
[9]郑国琴.,柳金凤.宁夏枸杞叶片组培快繁技术研究[J].北方园艺,2012,36(01):122.
ZHENG Guo-qin.,LIU Jin-feng.Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.[J].Northern Horticulture,2012,36(10):122.
[10]关丽霞.君子兰试管苗叶片植株再生的影响因素研究[J].北方园艺,2013,37(09):114.
GUAN Li-xia.Study on Factors Influencing Plant Regeneration of Clivia miniata Leaf[J].Northern Horticulture,2013,37(10):114.
备注/Memo
第一作者简介：张玉杰（1987-），女，黑龙江七台河人，硕士，研究方向为植物资源与种质创新。E-mail：1002097205@qq.com.