SUN Hai-yan,GUO Wei.Study on the Total Flavonoids Extrated from the Leaves of Sunflower by Orthogonal Test[J].Northern Horticulture,2013,37(03):16-18.
正交实验优选向日葵叶片提取总黄酮的研究
- Title:
- Study on the Total Flavonoids Extrated from the Leaves of Sunflower by Orthogonal Test
- 文章编号:
- 1001-0009(2013)03-0016-03
- Keywords:
- sunflower; leaf; total flavonoids; orthogonal test
- 分类号:
- S 565.5
- 文献标志码:
- A
- 摘要:
- 以向日葵叶片为试材，采用硝酸铝-亚硝酸钠比色法，以芦丁作为标准品，采用分光光度法测定了向日葵总黄酮的含量，并进行了精密度和加标回收试验，比较不同提取工艺以期优选出向日葵总黄酮的最佳提取工艺。结果表明：向日葵叶片中总黄酮最佳提取工艺为乙醇浓度60%，提取时间为3 h，提取温度为80℃。
- Abstract:
- Taking the leaves of sunflower as materials，totoal flavonoids in sunflower was determined by sodium nitrite-aluminum nitrate colorimetric with rutin as a standard substance.Precision and coefficient of recovery were discussed by studying total flavonoids content in sunflower，and different extraction process were compared in order to optimize optimum extraction process of total flavonoids content in sunflower.The results showed that the sunflower leaves flavonoids optimum extraction process were ethanol concentration 60%，extracting time 3 h，extraction temperature 80℃.
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备注/Memo
第一作者简介：孙海燕(1979-)，女，硕士，助理研究员，现主要从事农产品及食品分析和检测等的研究工作。E-mail：shysun7908@126.com.