LIU Yueqin,BAI Lu,LUO Liyuan,et al.Optimization of Heat Stress Conditions for the Synthesis of Ganoderic Acid[J].Northern Horticulture,2021,(17):119-124.[doi:10.11937/bfyy.20205306]
促进灵芝酸合成的热胁迫条件的优化
- Title:
- Optimization of Heat Stress Conditions for the Synthesis of Ganoderic Acid
- Keywords:
- Ganderma lucidum; ganoderic acid; heat stress; synthesis
- 文献标志码:
- A
- 摘要:
- 以灵芝(Ganoderma lucidum)为试材，采用单因素试验和正交实验方法，研究了不同热胁迫温度、热胁迫起始时间、热胁迫持续时间对灵芝酸合成的影响，并通过热激条件的优化提高灵芝酸的生物分成，以期为灵芝酸的高效生产提供参考依据。结果表明：不同的热胁迫温度、热胁迫起始时间及热胁迫持续时间均对灵芝酸的生物合成产生影响。通过正交实验筛选得到生产灵芝酸的最佳条件为热胁迫温度42 ℃，热胁迫起始时间为发酵的第7天，热胁迫持续时间为6 h。在该条件下，灵芝酸含量高，达88.23 mg·L-1，是未经过热胁迫处理的1.93倍。
- Abstract:
- The Ganderma lucidum was used as experimental material,the effects of different heat stress temperature,heat stress starting time and heat stress duration time on the synthesis of ganoderic acid were studied by single factor and orthogonal experimental methods,and the content of ganoderic acid through the optimization of heat shock conditions was improved,in order to provide some ideas and theoretical reference value for the efficient production of ganoderic acid.The results showed that the biosynthesis of ganoderic acid was affected by the different temperature,starting time and duration time of heat stress.Then the optimal conditions for the production of ganoderic acid were as follows,the heat stress temperature was 42 ℃,the starting time was 7th day and the duration time was 6 hours,which measured by the orthogonal experiment.Under this condition,the content of ganoderic acid was as high as 88.23 mg·L-1,which was 1.93 times higher than the control that without heating stress treatment.This study confirmed that the content of ganoderic acid can be improved through the optimization of heat stress conditions in liquid fermentation of Ganderma lucidum.
参考文献/References:
[1]BISHOP K S,KAO C H J,XU Y,et al.From 2000 years of Ganoderma lucidum to recent developments in nutraceuticals[J].Phytochemistry,2015,114:56-65.[2]RATHORE H,PRASAD S,SHARMA S.Mushroom nutraceuticals for improved nutrition and better human health a review[J].Pharma Nutrition,2017（5）:35-46.[3]李玲,孙元章,李刚.灵芝生物活性成分及其药理作用研究进展[J].南方农业,2019,13(4):50-55.[4]SHI L,REN A,MU D,et al.Current progress in the study on biosynthesis and regulation of ganoderic acids[J].Applied Microbiology and Biotechnology,2010,88(6):1243-1251.[5]PALIYA B S,VERMA S,CHAUDHARY H S.Major bioactive metabolites of the medicinal mushroom:Ganoderma lucidum[J].International Journal of Pharmaceutical Research,2014,6(1):13.[6]刘月芹,贺晓龙,任桂梅.较高温度对灵芝培养特性及灵芝酸产量的影响[J].微生物学杂志,2019,39(2):90-96.[7]REN A,SHI L,ZHU J,et al.Shedding light on the mechanisms underlying the environmental regulation of secondary metabolite ganoderic acid in Ganoderma lucidum using physiological and genetic methods[J].Fungal Genetics and Biology,2019,128:43-48.[8]HU G S,ZHAI M H Y,NIU R,et al.Optimization of culture condition for ganoderic acid production in Ganoderma lucidum liquid static culture and design of a suitable bioreactor[J].Molecules,2018(23):2563.[9]DONG Q Y,LI Y Y,LIU G Q,et al.High oxygen treatments enhance the contents of phenolic compound and ganoderic acid,and the antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body[J].Frontiers in Microbiology,2019(10):2363.[10]YANG C S,LI W C,LI C,et al.Metabolism of ganoderic acids by a Ganoderma lucidum cytochrome P450 and the 3-keto sterol reductase ERG27 from yeast[J].Phytochemistry,2018,155：83-92[11]XU J W,XU Y N,ZHONG J J.Enhancement of ganoderic acid accumulation by overexpression of an N-terminally truncated 3-hydroxy-3-methylglutaryl coenzyme a reductase gene in the basidiomycete Ganoderma lucidum[J].Applied and Environmental Microbiology,2012,78(22):7968-7976.[12]LAN X T,YUAN W,WANG M,et al.Efficient biosynthesis of antitumor ganoderic acid HLDOA using a dual tunable system for optimizing the expression of CYP5150L8 and a Ganoderma P450 reductase[J].Biotechnology and Bioengineering,2019,116:3301-3311.[13]GAO T,SHI L,ZHANG T J,et al.Cross talk between calcium and reactive oxygen species regulates hyphal branching and ganoderic acid biosynthesis in Ganoderma lucidum under copper stress[J].Applied and Environmental Microbiology,2018,84(13):e00438-18.[14]TIAN J L,REN A,WANG T,et al.Hydrogen sulfide,a novel small molecule signaling agent,participates in the regulation of ganoderic acids biosynthesis induced by heat stress in Ganoderma lucidum[J].Fungal Genetics and Biology,2019,130:19-30.[15]XU Y N,XIA X X,ZHONG J J.Induction of ganoderic acid biosynthesis by Mn2+ in static liquid cultivation of Ganoderma lucidum[J].Biotechnology and Bioengineering,2014,111(11):2358-2365.[16]ZHANG X,REN A,LI M J,et al.Heat stress modulates mycelium growth,heat shock protein expression,ganoderic acid biosynthesis,and hyphal branching of Ganoderma lucidum via cytosolic Ca2+[J].Applied and Environmental Microbiology,2016,82(14):4112-4125.[17]LIU R,ZHANG X,REN A,et al.Heat stress-induced reactive oxygen species participate in the regulation of HSP expression,hyphal branching and ganoderic acid biosynthesis in Ganoderma lucidum[J].Microbiological Research,2018,209:43-54.[18]解玉玲.高温胁迫对植物生理影响的研究进展[J].吉林农业,2019(8):107-108.[19]杨丽红.柑橘全爪螨Panonychus citri（McGregor）对热胁迫的响应机制研究[D].重庆:西南大学,2011.[20]廖飞雄,潘瑞炽.热胁迫下菜心脯氨酸含量变化及其在耐热中的作用[J].华南师范大学学报(自然科学版),2001(2):45-49.[21]张江萍,付丽娇,刘和.促进香菇多糖生物合成的热胁迫条件优化[J].食用菌学报,2018,25(1):42-46.[22]LIU Y Q,ZHANG G L,SUN H,et al.Enhanced pathway efficiency of Saccharomyces cerevisiae by introducing thermo-tolerant devices[J].Bioresource Technology,2014,170:38-44.[23]孙金旭.溶氧控制条件对深层灵芝发酵生产灵芝酸产量的影响[J].北方园艺，2014(5):130-132.[24]袁媛.高纯灵芝酸的分离纯化工艺研究[D].合肥:合肥工业大学,2007.[25]邢康康,刘艳,贺宗毅,等.灵芝酸研究进展[J].食用菌学报,2017,24(3):96-103.[26]杜萌,刘璇,陈彦,等.指纹图谱和体外细胞活性跟踪相结合考察温度对灵芝中三萜类成分的影响[J].中国实验方剂学杂志,2012(10):104-108.[27]韦会平,刘正宇,谭杨梅,等.温度条件对金佛山灵芝生长的影响[J].中国农学通报,2005,21(10):85-87.[28]魏巍,余梦瑶,许晓燕,等.出芝温度对灵芝基质物质含量和酶活的影响[J].中国食用菌,2014,33(6):49-55.[29]汪金萍,吴保锋.液体培养条件下温度对灵芝菌丝作用效果研究[J].北方园艺,2010(16):203-204.[30]CHETAN S,NEHA B,ANUPAM S,et al.Bioactive metabolites of Ganoderma lucidum:Factors,mechanism and broad spectrum potential[J].Journal of Herbal Medicine,2019(17):100268.
相似文献/References:
[1]王瑞霞,甄红伟,牟建楼,等.灵芝红枣复合饮料的研制[J].北方园艺,2013,37(04):151.
[2]朱会霞.不同培养方式对灵芝中麦角固醇产量的影响研究[J].北方园艺,2014,38(04):127.
ZHU Hui-xia.Effect of Different Fermentation Methods on the Yield of Ergosterol for Ganodorma lucidum [J].Northern Horticulture,2014,38(17):127.
[3]朱会霞.灵芝发酵覆盆子生产总黄酮的培养基优化研究[J].北方园艺,2013,37(15):169.
ZHU Hui-xia.Study on Optimization of Medium for Total Flavnoids Production from Rubus idaeus L. by GanodermaFermentation[J].Northern Horticulture,2013,37(17):169.
[4]杨海军,周小苹.辣椒粉固态培养灵芝菌丝体的培养基优化研究[J].北方园艺,2013,37(07):161.
YANG Hai-jun,ZHOU Xiao-ping.Study on Optimization of Solid Medium Containing Chili Powder for Ganoderma lucidum Culture[J].Northern Horticulture,2013,37(17):161.
[5]朱会霞.CO2超临界法萃取深层发酵灵芝真菌胞内灵芝酸的研究[J].北方园艺,2013,37(21):158.
ZHU Hui-xia.Study on the Optimization of Ganoderic Acids from Ganoderma lucidum After Fermentation by Supercritical Fluid Extraction Method[J].Northern Horticulture,2013,37(17):158.
[6]王昱,王义,王康宇,等.灵芝原生质体的制备与再生研究[J].北方园艺,2013,37(16):184.
WANGYu,WANGYi,WANGKang-yu,et al.StudyonPreparationandRegenerationoftheProtoplastsfrom Ganodermalucidum[J].Northern Horticulture,2013,37(17):184.
[7]李宇伟,连瑞丽,班强,等.灵芝液体发酵培养条件的响应面法优化研究[J].北方园艺,2012,36(13):179.
LI Yu-wei,LIAN Rui-li,BAN Qiang,et al.Optimization of Liquid Fermentation Conditions for Ganoderma Lucidum Using Response Surface Methodology[J].Northern Horticulture,2012,36(17):179.
[8]李海源,张亮,张美萍,等.日本灵芝灵芝酸发酵工艺的优化[J].北方园艺,2012,36(12):170.
LIHai-yuan,ZHANGLiang,ZHANGMei-ping,et al.OptimizationofFermentationTechnologyforGanodermaAcidofJapaneseGanoderma[J].Northern Horticulture,2012,36(17):170.
[9]柳焕章,卢月霞,郑素月.四种食药用真菌菌种扩繁培养基筛选[J].北方园艺,2014,38(17):145.
LIU Huan-zhang,LU Yue-xia,ZHENG Su-yue.Screening of Culture Medium of Four Different Edible-medicinal Fungi[J].Northern Horticulture,2014,38(17):145.
[10]马红梅,樊荣.灵芝菌糠提取液对灵芝菌丝体生长的化感效应[J].北方园艺,2015,39(15):136.[doi:10.11937/bfyy.201515036]
MA Hongmei,FAN Rong.Allelopathy of Extract of Residue of Ganoderma lucidum on Hyphal Growth of Ganoderma lucidum[J].Northern Horticulture,2015,39(17):136.[doi:10.11937/bfyy.201515036]
[11]朱会霞.灵芝真菌发酵产灵芝酸培养基优化研究[J].北方园艺,2013,37(22):154.
ZHU Hui-xia.Study on the Optimization of Culture Medium of Ganoderma Fermentation Before Extraction of Ganoderic Acid[J].Northern Horticulture,2013,37(17):154.
[12]孙金旭.溶氧控制条件对深层灵芝发酵生产灵芝酸产量的影响[J].北方园艺,2014,38(05):130.
SUN Jin-xu.Effect of Dissolved Oxygen Controlling Conditions on Production of Ganoderic Acids by Ganoderma[J].Northern Horticulture,2014,38(17):130.
备注/Memo
第一作者简介：刘月芹（1980-），女，博士，讲师，硕士生导师，现主要从事微生物资源的开发及食用菌栽培等研究工作。E-mail:liuyue811223@126.com.基金项目：延安大学博士科学研究启动资助项目（YDBK2015-08）；陕西省教育厅资助项目（20JC037）；延安大学产学研合作资助项目（CYFP201902）；陕西省科学技术厅资助项目（2020NY-078）。收稿日期：2020-12-14