WANGYu,WANGYi,WANGKang-yu,et al.StudyonPreparationandRegenerationoftheProtoplastsfrom Ganodermalucidum[J].Northern Horticulture,2013,37(16):184-188.
灵芝原生质体的制备与再生研究
- Title:
- StudyonPreparationandRegenerationoftheProtoplastsfrom Ganodermalucidum
- 文章编号:
- 1001-0009(2013)16-0184-05
- Keywords:
- Ganodermalucidum; protoplast; preparation; regeneration
- 分类号:
- S567.3+1
- 文献标志码:
- A
- 摘要:
- 以泰山灵芝为试材，研究了菌龄、酶浓度、酶解时间、酶解温度、酶解pH、渗透压稳定剂对灵芝原生质体制备和再生的影响。结果表明：菌龄为7d，以甘露醇为渗透压稳定剂，采用酶浓度2.0%的溶壁酶，pH为6.0，31℃条件下酶解2.5h为灵芝原生质体制备的最适条件；并在原生质体再生固体培养基（以0.6mol/L蔗糖为渗透压稳定剂）上，原生质体的再生率最高。
- Abstract:
- Taking Ganodermalucidum asthematerial，theeffectofTaishan Ganodermalucidum’s fungusage，enzymeconcentration，enzymesolutiontime，enzymesolutiontemperature，enzymesolutionpH，osmoticpressurestabilizeronplasmasystemandtheregenerationwerestudied.Theresultsshowedthatfungusagefor7days，withmannitolforosmoticpressurestabilizer，theenzymeconcentration2.0%，solublewallenzyme，pH6.0，undertheconditionoftheenzymesolution2.5hwastheoptimum;andinMYGmedium(0.6mol/Lsucroseforosmoticpressurestabilizer)，protoplastregenerationhadthehighestrate.
参考文献/References:
[1]孙金旭,张卿,朱会霞.优化灵芝真菌发酵条件的研究[J].中国酿造,2008（4）：27-29.
[2]于智勇.灵芝-仙草[J].生物学杂志,1995(3):25.
[3]VriesdeOMH，WesselsJGH.ReleaseofprotoplastsfromSchizopHyllumcommunebyalyticenzymepreparationfromTrichodermaviride[J].JGenMicrobiol,1972,73:13-22.
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备注/Memo
第一作者简介：王昱（1987-），女，硕士研究生，研究方向为植物细胞工程。E-mail:247989080@qq.com.
责任作者：张美萍（1966-），女，博士，教授，研究方向为药用植物细胞工程。E-mail:wzhaoyun@tom.com.
收稿日期：2013-04-24