WANG Yongyuan,LI Yanlong,XU Hongxia,et al.Effects of Different Carbon and Nitrogen Sources on Mycelial Growth and Sclerotia Formation of Morchella spp.[J].Northern Horticulture,2021,(17):125-131.[doi:10.11937/bfyy.20211661]
不同碳源、氮源对羊肚菌菌丝生长及菌核形成的影响
- Title:
- Effects of Different Carbon and Nitrogen Sources on Mycelial Growth and Sclerotia Formation of Morchella spp.
- Keywords:
- Morchella spp.; carbon source; nitrogen source; mycelium culture; sclerotium
- 文献标志码:
- A
- 摘要:
- 以羊肚菌六妹菌株6611和梯棱菌株M2018为供试菌株，采用菌丝培养的方法，研究了11种碳源、7种氮源对2种菌株菌丝生长、菌核形成的影响,以期为羊肚菌的菌种制备、液体培养条件优化以及相关功能性保健产品的开发利用提供参考依据。结果表明：2种菌株的菌丝在供试的碳源、氮源中均能生长。其中菌株6611，在麦芽糖、葡萄糖、甘露醇、山梨醇、D-半乳糖、硝酸钠、酒石酸铵培养基中菌丝长势较好；在山梨醇、乳糖、D-半乳糖、淀粉、硝酸钠、酒石酸铵培养基中菌核形成较多且以近边缘分布的黄色、褐色小颗粒的形态居多。菌株M2018，在麦芽糖、淀粉、葡萄糖、乳糖、硝酸钠、磷酸氢二铵、酒石酸铵培养基中菌丝长势较好；在山梨醇、D-核糖、D-半乳糖、硝酸钠、磷酸氢二铵、酒石酸铵培养基中菌核形成较多且以分散分布、近边缘分布的黄色、褐色球状或块状的形态居多，在尿素培养基中无菌核形成。
- Abstract:
- Morchella sextelata strain 6611 and Morchella importuna strain M2018 were selected for testing.Using the method of mycelium culture,the effects of 11 types of carbon sources and 7 types of nitrogen sources on the hyphal growth and sclerotial formation of the two strains were studied,in order to provide a reference for preparing Morchella spp.strains,optimize liquid culture conditions,and develop related functional health products.The results showed that the hyphae of the two strains could grow in the tested carbon and nitrogen sources.In particular,strain 6611 showed good hyphal growth in the medium containing maltose,glucose,mannitol,sorbitol,D-galactose,sodium nitrate,and ammonium tartrate.In the medium containing sorbitol,lactose,D-galactose,starch,sodium nitrate,and ammonium tartrate,however,sclerotia were formed more and mostly in the form of small yellow or brown particles distributed near the edge.Strain M2018 showed good hyphal growth in the medium containing maltose,starch,glucose,lactose,sodium nitrate,diammonium hydrogen phosphate,and ammonium tartrate.In the medium containing sorbitol,D-ribose,D-galactose,sodium nitrate,diammonium hydrogen phosphate,and ammonium tartrate,sclerotia were formed more and mostly in the form of yellow or brown spheres and blocks distributed near the edge.In the urea medium,no sclerotium was formed.
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备注/Memo
第一作者简介：王永元（1987-），男，本科，工程师，现主要从事羊肚菌人工栽培推广等研究工作。E-mail:1004525921@qq.com.责任作者：曹秀文（1964-），男，硕士，正高级工程师，现主要从事林业科研和食用菌等研究工作。E-mail:GSCAOXW@126.com.基金项目：中央财政林业科技推广示范资助项目（2019ZYTG10）；甘肃省林业科技资助项目（2017kj040）。收稿日期：2021-04-16