FU Yunliu,XU Li,LI Zhiying,et al.In vitro Culture and Plant Regeneration of Ardisia gigantifolia Stapf.[J].Northern Horticulture,2017,41(04):98-101.[doi:10.11937/bfyy.201704022]
走马胎离体培养及植株再生
- Title:
- In vitro Culture and Plant Regeneration of Ardisia gigantifolia Stapf.
- 文献标志码:
- A
- 摘要:
- 以走马胎幼嫩叶片为外植体，研究不同培养基对走马胎叶片愈伤组织诱导、不定芽分化、增殖及生根培养的影响。结果表明：叶片在MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1培养基上诱导产生的愈伤组织最适合用于进行分化和增殖；愈伤组织分化不定芽的最适培养基为MS+6-BA 2.0 mg?L-1+NAA 0.1 mg?L-1，分化率高达88.9%；壮苗培养基为MS+6-BA 〖JP〗0.5 mg?L-1+NAA 0.1 mg?L-1+10%椰子水(CW)；最适的生根培养基为MS+NAA 0.1 mg?L-1+IBA 1.0 mg?L-1，生根率达100%，根系发达，植株生长健壮。经生根培养及练苗，走马胎的假植成活率达85%。
- Abstract:
- A.gigantifolia Stapf.leaves were used as explants，the effects of different mediums on leaf callus induction，adventitious bud differentiation，proliferation and rooting were studied.The results showed that the callus induced from leaf explants on medium MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1 were easy to produce auxiliary shoots.The differentiation rate was 88.9%.The suitable medium for bud subculture and proliferation was MS+6-BA 2.0 mg?L-1+NAA 0.1 mg?L-1.The elongation medium was MS+6-BA 0.5 mg?L-1+NAA 0.1 mg?L-1+10% coconut water.The optimal rooting medium was MS+NAA 0.1 mg?L-1+IBA 1.0 mg?L-1 with rooting rate as high as 100%.The roots developed well and the plantlets grew vigorously.The plantlets with well-developed roots were successfully acclimatized and planted with a survival rate of 85%.
参考文献/References:
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备注/Memo
第一作者简介：符运柳(1978-)，女，硕士，助理研究员，现主要从事植物种质资源保存等研究工作。E-mail:fyljj_2007@126.com.责任作者：李志英（1971-），女，博士，研究员，研究方向为植物种质资源保存与利用。E-mail:xllizhiying@vip.163.com.基金项目：农业部引进国际先进技术资助项目（2015-Z22）；农业部物种资源保护专项资助项目（16RZZY-101）。