FANG Shi-mei,LEI Shi-jun,WANG Hong-bo,et al.Study on Tissue Culture and Rapid Propagation of Salvia[J].Northern Horticulture,2013,37(21):123-126.
丹参组培快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation of Salvia
- 文章编号:
- 1001-0009(2013)21-0123-04
- Keywords:
- Salvia; tissue culture; rapid propagation
- 分类号:
- S 567.5+3
- 文献标志码:
- A
- 摘要:
- 以紫花丹参茎尖、茎段、叶片、花蕾为试材，采用随机排列的试验设计，研究了不同灭菌时间、不同激素水平对丹参愈伤组织形成、丛生芽分化、试管苗生根、试管苗驯化移栽的影响。结果表明：茎段用0.1% HgCl2溶液灭菌10 min效果最好，成活率最高可达90%以上；丹参小苗顶芽在MS+6-BA 1.0 mg/L+NAA 0.1 mg/L中诱导丛生芽效果最好；试管苗生根以1/2MS+NAA 0.4 mg/L生根效果最好，生根率最高可达95%以上；试管苗的驯化移栽以蛭石＋珍珠岩的组合既保水又透气，最有利于丹参的生根，成活率可达98%。
- Abstract:
- Taking the explants of the stem tips，stem fragment，leaf，bud of Salvia as materials，the effect of different sterilizer time，different hormone levels on callus formation，buds differentiation，rooting，and plantlets acclimatization of Salvia were studied by random permutation test design method.The results showed that stems sterilized with 0.1% HgCl2 solution for 10 min could reach the highest survival rate of more than 90%；Salvia seedlings buds in MS+6-BA 1.0 mg/L+NAA 0.1 mg/L could get the best effect；on 1/2MS+NAA 0.4 mg/L the plantlets had the best rooting rate of more than 95%；plantlets acclimatization to a combination of vermiculite and perlite both water and breathable was more favorable for Salvia rooting，the survival rate could up to 98%.
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备注/Memo
第一作者简介：房师梅（1967-），女，本科，副教授，现主要从事植物组织培养工作。E-mail:fangshimei@126.com.