LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(01):106-108.
黄山贡菊茎尖脱毒快繁技术研究
- Title:
- Tissue Culture and Rapid Propagation of Chrysanthemum morifolium
- 文章编号:
- 1001-0009（2014）01-0106-03
- Keywords:
- Chrysanthemum morifolium; stem tip; tissue culture; rapid propagation
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以黄山贡菊茎段为外植体，研究了不同消毒方法和不同培养基对黄山贡菊茎尖组织培养的影响。结果表明：0.1%升汞消毒8 min，外植体污染率及褐化率较低；诱导茎尖分化的最适培养基为MS+6-BA 1.0 mg/L +NAA 0.05 mg/L，芽诱导率可高达100%，且丛生芽生长较快；最适宜生根的培养基为MS+NAA 0.5 mg/L，植株生根粗壮，根系发达，利于练苗及移栽。
- Abstract:
- With the stem tips of Chrysanthemum morifoliumas the explants,the effects of different disinfection methods and culture mediums on stem tip tissue culture were studied.The results showed that the contamination rate and browning rate of the explants were the lowest by 0.1% mercuric chloride for 8 min.The optimum culture medium for inducing adventitious shoots was MS+6-BA 1.0 mg/L +NAA 0.05 mg/L,the bud induction rate was up to 100%,and the multiple shoots grow rapidly；the optimum rooting culture medium was MS+NAA 0.5 mg/L,it was easier for acclimatizing and transplanting with the sturdy root system.
参考文献/References:
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备注/Memo
第一作者简介：李云玲（1982-），女，硕士，讲师，现主要从事农业生物技术等研究工作。E-mail:liyunling82@126.com.