LI Jinghong,LIU Yanli.Effect of Culture Conditions on Explants Browning of Ficus carica L.[J].Northern Horticulture,2015,39(14):99-101.[doi:10.11937/bfyy.201514026]
无花果组培中培养条件对外植体褐化的影响
- Title:
- Effect of Culture Conditions on Explants Browning of Ficus carica L.
- Keywords:
- Ficus carica L.; tissue culture; browing
- 文献标志码:
- A
- 摘要:
- 以无花果为组织培养试验材料，采用改变培养条件和使用不同培养基的方法，研究培养条件对外植体褐化的影响。结果表明：无花果外植体褐化在WPM培养基上比MS培养基上较轻，培养基中琼脂为9 g的无花果外植体褐化较轻，经过暗处理的无花果外植体褐化程度较轻，以及低温条件能有效缓解外植体褐化。
- Abstract:
- Taking Ficus carica L.as a tissue culture experiment material,adopting the method of changing culture conditions and using different culture medium，the effect of culture conditions on the explants browning was studied.The results showed that on WPM medium the explant browning was lighter than on MS medium；the explant browning was lighter on 9 g agar medium and under dark treatment;low temperature could effectively alleviate the explant browning.
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备注/Memo
第一作者简介：李景蕻（1967-），男，博士，副教授，研究方向为植物学及其应用。E-mail：15527608267@163.com.基金项目：湖北第二师范学院“植物抗癌活性物质提纯与应用湖北省重点实验室”建设资助项目。