ZOU Lijuan,WU Qinggui,XIE Mushan,et al.Establishment of Regeneration System of Atropa belladonna[J].Northern Horticulture,2015,39(14):102-105.[doi:10.11937/bfyy.201514027]
颠茄植株再生体系的建立
- Title:
- Establishment of Regeneration System of Atropa belladonna
- Keywords:
- Atropa belladonna; callus; differentiation; plant regeneration
- 文献标志码:
- A
- 摘要:
- 以颠茄种子萌发的无菌苗为试材，采用组织培养方法，以MS为基本培养基，研究IAA、NAA、6-BA、KT对愈伤组织诱导及分化的影响，探索适宜颠茄愈伤组织诱导和分化的培养基。结果表明：在MS+KT 0.5 mg/L+6-BA 1.0 mg/L和MS+KT 1.0 mg/L+6-BA 0.5 mg/L培养基上，愈伤诱导率较高达100%，KT、6-BA添加生长素配比诱导愈伤组织效果不佳。KT单独诱导的愈伤组织宜在20 d后转接，KT和6-BA配合使用诱导出的愈伤组织宜在40 d后转接。生根适宜培养基为1/2MS+NAA 0.2～0.4 mg/L。该研究建立了颠茄的植株再生体系，为颠茄的组织培养提供了技术体系。
- Abstract:
- Taking seedlings of Atropa belladonna as explants，by tissue culture methods，MS as basic culture medium with different concentrations of IAA，NAA，6-BA and KT were used to establish the optimal media for callus induction and differentiation.The results showed that 100% of calli induction rate was obtained on MS medium with KT 0.5 mg/L+6-BA 1.0 mg/L and KT 1.0 mg/L+6-BA 0.5 mg/L.Calli could not be induced on MS medium with the combination of auxin and KT or 6-BA.The induced calli on MS media with KT or KT with 6-BA were subcultured optimally at 20 days and 40 days.1/2MS+NAA 0.2-0.4 mg/L was the optimal medium for plants’ rooting.This study would provide the technologicalsystem of tissue culture for Atropa belladonna regeneration.
参考文献/References:
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备注/Memo
第一作者简介：邹利娟（1983-），女，硕士，讲师，现主要从事植物生理及植物组织培养等研究工作。E-mail：ljzou66@163.com.责任作者：罗明华（1964-），男，博士，教授，现主要从事药用植物资源学等研究工作。E-mail：mhemei@126.com.基金项目：国家自然科学基金资助项目（31100246）；四川省科技厅资助项目（2013JY0078）；四川省教育厅资助项目（13ZB0120,13ZB0121）；绵阳师范学院校级资助项目(2012A08，2011A09)。