QIAO Gaixia,CHEN Chunling,XU Meilong,et al.Study on Tissue Culture of Amygdalus tangutica[J].Northern Horticulture,2015,39(14):96-98.[doi:10.11937/bfyy.201514025]
四川扁桃组织培养技术研究
- Title:
- Study on Tissue Culture of Amygdalus tangutica
- Keywords:
- Amygdalus tangutica; tissue culture; plant regeneration
- 文献标志码:
- A
- 摘要:
- 四川扁桃是一种具有良好经济和生态价值的灌木树种，开发利用前景广阔。以四川扁桃一年生嫩枝切段为试材，采用外植体芽诱导的方法，研究不同培养基对四川扁桃组织培养的影响。结果表明：适合四川扁桃外植体芽诱导培养基为MS+6-BA 0.8 mg／L+IAA 1.5 mg/L+NAA 0.01 mg／L+蔗糖30 g/L+琼脂7 g/L，分化培养基为MS+6-BA 0.4 mg／L+IAA 0.8 mg/L+NAA 0.01 mg／L+蔗糖30 g/L+琼脂7 g/L，生根培养基为1/2MS+IBA 0.5 mg/L+蔗糖30 g/L+琼脂7 g/L。
- Abstract:
- Amygdalus tangutica is a kind of shrub that is good for its economical and ecological value，which has a good future of development and application.Using annual tender stems of Amygdalus tangutica as explants to research the effect of different medium on tissue culture of Amygdalus tangutica.The results showed that the proper initiation culture medium was MS+6-BA 0.8 mg/L+IAA 1.5 mg/L+NAA 0.01 mg/L+sucrose 30 g/L+agar 7 g/L，the proper multiplication culture medium was MS+6-BA 0.4 mg/L+IAA 0.8 mg/L+NAA 0.01 mg/L+sucrose 30 g/L+agar 7 g/L，the proper rooting culture medium was 1/2MS+IBA 0.5 mg/L+sucrose 30 g/L+agar 7 g/L.
参考文献/References:
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备注/Memo
第一作者简介：乔改霞（1988-），女，陕西榆林人，本科，现主要从事植物组织培养等研究工作。E-mail：534790912@qq.com.责任作者：陈春伶（1983-），女，硕士,助理研究员，现主要从事植物生物技术等研究工作。基金项目：国家林业行业公益专项资助项目（201104041）。