ZHOU Jin-mei,YE Fei,JIAN De-feng.In vitro Rapid Propagation of Rhododendron dauricum Leaf[J].Northern Horticulture,2012,36(21):98-100.
兴安杜鹃叶片离体培养技术研究
- Title:
- In vitro Rapid Propagation of Rhododendron dauricum Leaf
- 文章编号:
- 1001-0009（2012）21-0098-03
- Keywords:
- Rhododendron dauricum L; leaf; medium; culture in vitro
- 分类号:
- S 685.21
- 文献标志码:
- A
- 摘要:
- 以兴安杜鹃的叶片为外植体，研究其离体培养技术。结果表明：最佳愈伤组织诱导培养基为1/4Anderson+2,4-D 1 mg/L+TDZ 0.1 mg/L，最佳芽诱导培养基为1/4Anderson+IBA 0.1 mg/L+TDZ 1 mg/L+2-IP 0.5 mg/L，最佳生根培养基为1/4Anderson+IBA 2 mg/L或1/4 Anderson+NAA 0.1 mg/L。
- Abstract:
- Taking the leaves as material，the rapid propagation culture in vitro of Rhododendron dauricum were studied.The results showed that the best callus induction medium was 1/4Anderson+2,4-D 1 mg/L+TDZ 0.1 mg/L；the best bud induction medium was 1/4Anderson+IBA 0.1 mg/L+TDZ 1 mg/L+2-IP 0.5 mg/L；the best rooting culture medium was 1/4Anderson+IBA 2 mg/L or the medium of 1/4Anderson+NAA 0.1 mg/L.
参考文献/References:
[1]任宪威.树木学[M].北京:中国林业出版社,2006:264. [2]孙杨吾,任雪芹,朱元娣,等.不同生长调节物质对迎红杜鹃组培快繁的影响[J].北方园艺,2011(6):149-151. [3]朱春艳,李志炎,鲍蠢松,等.云锦杜鹃组培快繁技术研究[J].中国农学通讯,2006(5):68. [4]高文强,樊金会,赵红霞,等.杜鹃花组培快繁技术的研究[J].山东林业科技,2011(5):47. [5]徐颖,金灿,宗成文,等.长白山牛皮杜鹃组培快繁及初步驯化栽培研究[J].江苏农业科学,2010(2):88-90.
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备注/Memo
第一作者简介：周金梅（1976-），女，吉林省吉林市人，硕士，讲师，研究方向为园艺植物育种与栽培。E-mail:82642444@qq.com.