[1]陈曦,颉刚刚,刘柚藓,等.野生欧洲李叶片愈伤组织诱导及增殖培养体系研究[J].北方园艺,2020,44(24):1-7.[doi:10.11937/bfyy.20200750]
　CHEN Xi,XIE Ganggang,LIU Youxian,et al.Study on Callus Induction and Proliferation Culture System of Prunus domestica L.[J].Northern Horticulture,2020,44(24):1-7.[doi:10.11937/bfyy.20200750]
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野生欧洲李叶片愈伤组织诱导及增殖培养体系研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年24 页码: 1-7 栏目: 出版日期: 2020-12-30
Title:
Study on Callus Induction and Proliferation Culture System of Prunus domestica L.
作者:
陈曦; 颉刚刚; 刘柚藓; 经建永; 马百强; 耿文娟
（新疆农业大学 林学与园艺学院，新疆 乌鲁木齐 830052）
Author(s):
CHEN Xi; XIE Ganggang; LIU Youxian; JING Jianyong; MA Baiqiang; GENG Wenjuan
(College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi,Xinjiang 830052)
关键词:
野生欧洲李; 叶片; 愈伤组织; 组织培养
Keywords:
Prunus domestica L.; leaves; callus; tissue culture
DOI:
10.11937/bfyy.20200750
文献标志码:
A
摘要:
以野生欧洲李叶片为试材，采用组织培养的方法，研究了消毒时间、暗处理时间、基础培养基种类、生长调节剂种类及浓度等影响野生欧洲李叶片愈伤组织诱导及增殖的因素筛选，以期为建立高效野生欧洲李培养体系提供参考依据。结果表明：消毒时间70%酒精处理40 s+0.1%氯化汞6 min处理后叶片的污染率最低为6.67%，死亡率为0%。暗处理28 d时叶片所形成的愈伤组织诱导率最高可达80.5%，叶脉处膨大显著，脱分化形成愈伤组织。叶片在B5培养基中愈伤组织诱导率最大为75.56%。生长调节剂对叶片所形成的愈伤组织诱导率最大可达86.67％。野生欧洲李叶片愈伤组织诱导的最适培养方法为先用70%的酒精消毒40 s，无菌水清洗2~3次，再用0.1%氯化汞溶液浸泡消毒6 min，无菌水清洗4~5次。最佳暗处理时间为14 d。初代培养的较优培养基配方为B5+7.5 mg?L-1 TDZ+2.5 mg?L-1 IBA。
Abstract:
The leaves of Prunus domestica L.were used as experimental materials in this study.The factors affecting callus induction and proliferation of Prunus domestica L.leaves,such as disinfection time,dark treatment time,type of basic medium,type and concentration of growth regulators and so on,were screened.In order to provide a basis for establishing a highly efficient culture system of Prunus domestica L..The results showed that disinfection time 70% alcohol treatment 40 seconds+0.1% mercuric chloride 6 minutes treatment,the lowest contamination rate of leaves was 6.67%,and the mortality rate was 0%.After 28 days of dark treatment,the callus induction rate of leaves was up to 80.5%,and the leaf veins swelled significantly and dedifferentiated to form calli.The maximum callus induction rate of leaves in B5 medium was 75.56%.The maximum callus induction rate of leaves formed by growth regulators was 86.67%.The optimum culture method for callus induction of Prunus domestica L. leaves was to disinfect 40 seconds with 70% alcohol and wash it with sterile water for 2-3 times.Then soak in 0.1% mercuric chloride solution for 6 minutes and wash it with sterile water for 4-5 times.The best dark treatment time was 14 days.The better medium formula for primary culture was B5+7.5 mg?L-1 TDZ+2.5 mg?L-1 IBA.

参考文献/References:

[1]林培钧,崔乃然.天山野果林资源：伊犁野果林综合研究[M].北京:中国林业出版社,2000.[2]耿文娟.野生欧洲李种质资源特性及亲缘关系研究[D].乌鲁木齐:新疆农业大学,2011.[3]张加延,周恩.中国果树志?李卷[M].北京:中国林业出版社,1998.[4]胡柏文,车凤斌,郝秀英,等.新疆酸梅及其栽培利用[J].北方果树,2004(6):29-30.[5]冯涛,张红,陈学森,等.新疆野苹果果实形态与矿质元素含量多样性以及特异性状单株[J].植物遗传资源学报,2006(3):3270-3276.[6]高敏霞,叶新福.植物组织培养在核果类果树上的应用研究进展[J].中国热带农业,2015(3):62，107-108.[7]张悦,傅建敏,李福海,等.蔷薇科核果类果树组织培养研究进展[J].经济林研究,2011,29(1):155-160.[8]丛芳,韩明玉,赵彩平,等.桃叶片再生不定芽的研究[J].果树学报,2009,26(5):614-618,762.[9]姚延兴.李离体再生体系的建立及愈伤组织分化相关microRNA分析[D].武汉:华中农业大学,2011.[10]张陆阳.李叶柄（叶片）离体培养与植株再生的研究[D].武汉:华中农业大学,2009.[11]王鸿.杏的再生体系建立的研究[D].杨凌:西北农林科技大学,2005.[12]BARBARA N,KAZIMIEERZ M,LUDWIK H.The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘Wegierka Zwykla’ plum (Prunus domestica L.)[J].Acta Physiologiae Plantarum,2007,29(5):479-484.[13]耿文娟,吴玉霞,袁海英,等.野生欧洲李组织培养技术[J].经济林研究,2009,27(1):45-48.[14]吴玉霞,袁海英,曹恒,等.野生欧洲李试管苗生根与移栽技术研究[J].新疆农业科学,2009,46(1):87-90.[15]颉刚刚.野生欧洲李叶片愈伤组织诱导研究初报[C].青岛：中国园艺学会2018年学术年会,2018.[16]庄丽娟.欧李快速繁殖技术体系研究[D].呼和浩特:内蒙古农业大学,2005.[17]师校欣,杜国强,高仪,等.黑暗培养对苹果组培快繁及叶片再生的影响[J].河北农业大学学报,2004(4):18-21.[18]刘子花,李晓蓉,杨玲,等.Hort16A猕猴桃叶片直接再生不定芽研究[J].林业调查规划,2016,41(3):87-91.[19]ZHAO Y,STILES A R,SAXENA P K,et al.Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants[J].Biological Plantarum,2013,57(1):189-192.[20]宋春晖,谢贝阳,董志丹,等.柱状苹果组培外植体褐化控制与不定芽诱导方法探索[J].分子植物育种,2020,18(16):5459-5465.[21]周宜君,周生闯,刘玉,等.植物生长调节剂对植物愈伤组织的诱导与分化的影响[J].中央民族大学学报,2007,16(1):23-27.[22]浦艳吉,李国怀,姚延兴.李茎尖离体培养与植株再生[J].华中农业大学学报,2009,28(2):214-217.[23]VALERO M,IBANEZ A,MORTE A.Effect of high vineyard temperatures on the grapevine leafroll associated virus elimination from Vitis vinifera L.cv.Napoleon tissue cultures[J].Scientia Horticulture,2003,97(3):289-296.[24]徐华松,徐九龙,黄学林.TDZ在植物组织培养中的作用[J].广西植物,1996(1):77-80.[25]刘崇琪,陈学森,吴传金,等.新疆野生樱桃李(Prunus cerasifera)茎段与叶片培养及其植株再生[J].果树学报,2008(1):49-53,144.[26]武莎莎.欧李愈伤组织诱导及不定芽再生体系的优化[D].晋中:山西农业大学,2018.[27]何碧珠,陈振光.奈的离体繁殖[J].福建农业大学学报,1998,27(3):296-300.[28]FASOLO F.Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars[J].Plant Cell,Tissue and Organ Culture,1989,16(2):75-87.[29]BARBARA N,KAZIMIERZ M,LUDWIK H.Suger uptake and util-isation during adventitious bud differentiation on in vitro leaf explants of ‘Wegierka Zwykla’ plum (Prunus domestica)[J].Plant Cell Tissue and Organ Culture,2004,76:255-260.

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备注/Memo

第一作者简介：陈曦（1995-），女，硕士研究生，研究方向为果树种质资源。E-mail：824386171@qq.com.责任作者：耿文娟（1983-），女，博士，副教授，现主要从事果树种质资源与栽培等研究工作。E-mail：gwj0526@163.com.基金项目：国家自然科学基金资助项目（31560545）；国家重点研发计划资助项目（2016YFC0501504）；新疆维吾尔自治区园艺重点学科资助项目（2016-10758-3）。收稿日期：2020-03-03

更新日期/Last Update: 2021-02-19