[1]陈松树,张雪,赵致,等.以叶片为外植体的多花黄精组织培养[J].北方园艺,2018,42(14):136-142.[doi:10.11937/bfyy.20180510]
　CHEN Songshu,ZHANG Xue,ZHAO Zhi,et al.Tissue Culture of Polygonatum cyrtonema Huad.With Leaf as Explants[J].Northern Horticulture,2018,42(14):136-142.[doi:10.11937/bfyy.20180510]
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以叶片为外植体的多花黄精组织培养

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年14 页码: 136-142 栏目: 出版日期: 2018-07-25
Title:
Tissue Culture of Polygonatum cyrtonema Huad.With Leaf as Explants
作者:
陈松树1; 2; 张雪3; 赵致1; 3; 刘红昌1; 3; 王华磊1; 3; 李金玲1; 3
（1.贵州省药用植物繁育与种植重点实验室，贵州 贵阳 550025；2.贵州大学 教学实验场，贵州 贵阳 550025；3.贵州大学 农学院，贵州 贵阳 550025）
Author(s):
CHEN Songshu1; 2; ZHANG Xue3; ZHAO Zhi1; 3; LIU Hongchang1; 3; WANG Hualei1; 3; LI Jinling1; 3
(1.Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang,Guizhou 550025;2.Teaching Farm,Guizhou University,Guiyang,Guizhou 550025；3.Agricultural College,Guizhou University,Guiyang,Guizhou 550025)
关键词:
多花黄精; 叶片; 组织培养
Keywords:
Polygonatum cyrtonema Huad.; leaf; tissue culture
DOI:
10.11937/bfyy.20180510
文献标志码:
A
摘要:
以叶片为外植体建立多花黄精组织培养快速繁殖技术体系，通过添加不同浓度6-BA、NAA、2,4-D和蔗糖的MS培养基中培养，筛选愈伤组织诱导、愈伤组织增殖、丛生芽诱导、丛生芽生根诱导的最佳培养基。结果表明：愈伤组织诱导的最佳培养基为MS+6-BA 3.0 mg·L-1+NAA 4.0 mg·L-1+蔗糖20 g·L-1，愈伤组织诱导率最高为53.89%，生长情况最好；愈伤组织增殖培养的最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 3.0 mg·L-1+蔗糖20 g·L-1，增殖倍数7.87，生长情况相对最好；丛生芽诱导培养的最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 4.0 mg·L-1，出芽率最高为86.11%，生长情况最好，芽高最大为1.70 cm；丛生芽生根诱导的最佳培养基为MS+NAA 1.0 mg·L-1，生根率最高为66.67%，根数、根长和根粗最好，分别为31.0条、0.46 cm和1.100 mm。以叶片为外殖体可短时间获得大量多花黄精组培苗，解决种植生产上的种苗短缺问题。
Abstract:
To construct a rapid propagation system of the tissue culture of Polygonatum cyrtonema Huad.which regeneration from the leaves.The aim was to screen the optimum combination for the best medium of callus induction,callus proliferation,cluster bud induction and rooting induction of clustered shoots by adding different 6-BA,NAA,2,4-D and sucrose proportions in MS medium.The results showed that the best medium for callus induction was MS+6-BA 3.0 mg·L-1+NAA 4.0 mg·L-1+sucrose 20 g·L-1,and the callus induction rate was the highest,it reached 53.89%,and the growth was the best.The best medium for callus proliferation was MS+6-BA 2.0 mg·L-1+NAA 3.0 mg·L-1+sucrose 20 g·L-1,and the multiplication multiple was 7.87,and the growth was the best.The best medium for the induction culture of cluster buds was MS+6-BA 2.0 mg·L-1+NAA 4.0 mg·L-1,and the germination rate was the highest,it reached 86.11%,and the maximum height of 1.70 cm.The best medium of rooting induction for clustered shoots was MS+NAA 1.0 mg·L-1,and the rooting rate was the best,it reached 66.67%,and the best root number,length and diameter were 31.0,0.46 cm and 1.100 mm,respectively.A large amount of Polygonatum cyrtonema Huad.tissue culture seedlings could be obtained by the regeneration from the leaves,in a short time,which could solve the shortage of seedlings in planting production.

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备注/Memo

第一作者简介：陈松树（1987-）,男，硕士，助理实验师，研究方向为药用植物栽培与开发利用。E-mail：sschen1@gzu.edu.cn.责任作者：王华磊（1979-）,男，博士，教授，研究方向为药用植物栽培与开发利用。E-mail:whlgu@163.com.基金项目：国家自然科学基金资助项目（31760421）;贵州省作物学重点学科建设计划资助项目（黔学位合字ZDXK[2014]8号）;贵州省药用植物繁育与种植人才基地资助项目（黔人领发[2013]15号）;贵州省普通高等学校粮油作物遗传改良与生理生态特色重点实验室资助项目（黔教合KY字[2015]333）;贵州省生物学一流学科建设资助项目（GNYL[2017]009）;贵州省科技计划资助项目（黔科合平台人才[2017]5788号）。收稿日期：2018-03-12

更新日期/Last Update: 2018-08-20