XIONG Yi,WANG Honghong,ZHANG Fangsong,et al.Establishment of Protein Mulberry Leaves’ Regeneration System and Sensitivity Test of Phosphinothricin[J].Northern Horticulture,2016,40(17):98-102.[doi:10.11937/bfyy.201617023]
蛋白桑叶片再生体系的建立及对草丁膦敏感性测定
- Title:
- Establishment of Protein Mulberry Leaves’ Regeneration System and Sensitivity Test of Phosphinothricin
- Keywords:
- protein mulberry; leaves; regeneration system; phosphinothricin
- 文献标志码:
- A
- 摘要:
- 以蛋白桑的幼嫩叶片为外植体，以MS为基本培养基，采用正交实验设计，研究不同浓度的植物生长调节剂对蛋白桑植株再生的影响。结果表明：叶片诱导愈伤组织的最佳培养基为MS+TDZ 1.0 mg?L-1+NAA 0.1 mg?L-1；最佳分化培养基为MS+TDZ 0.2 mg?L-1+NAA 0.09 mg?L-1，分化率为21.65%；培养基1/2 MS+PVP 0.2 g?L-1+NAA 0.5 mg?L-1生根率最高，达到66.70%；对草丁膦的敏感性试验中，草丁膦浓度0.04 mg?L-1为蛋白桑叶片半致死浓度，草丁膦浓度0.05 mg?L-1对蛋白桑叶片的致死率为75.00%，草丁膦浓度0.07 mg?L-1的致死率为100.00%。
- Abstract:
- Taking young protein mulberry leaves as explants，MS as the basic medium，the effect of plant growth regulator with different concentrations on the protein mulberry regeneration was studied.The results showed that the best medium of leaf induce callus was MS+TDZ 1.0 mg?L-1+NAA 0.1 mg?L-1；the best differentiation medium was MS+TDZ 0.20 mg?L-1+NAA 0.09 mg?L-1，and the bud differentiations rate was 21.65%；medium of 1/2 MS+PVP 0.2 g?L-1+NAA 0.5 mg?L-1 got the highest rooting rate about 66.70%.And the results of phosphinothricin sensitivity test experiment showed that protein mulberry leaves’ lethality was 50.00%，75.00%，100.00% when concentration of phosphinothricin was 0.04 mg?L-1，0.05 mg?L-1 and 0.07 mg?L-1,respectively.
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备注/Memo
第一作者简介：熊意(1992-)，男，硕士研究生，研究方向为植物基因工程。E-mail:xiongyi1702@163.com.责任作者：祝建波(1967-)，男，博士，副研究员，现主要从事植物基因工程等研究工作。E-mail：zjbshz@163.com.基金项目：兵团创新团队资助项目(2014CC005);科技型中小企业创新基金资助项目(14C26216513822)。