[1]汪洪,夏鸿亮,李校堃,等.胡萝卜愈伤组织诱导培养的研究[J].北方园艺,2012,36(06):103-105.
　WANG Hong,XIA Hong-liang,LI Xiao-kun,et al.A Study on Calli Induction and Culture from Daucus carota L.[J].Northern Horticulture,2012,36(06):103-105.

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胡萝卜愈伤组织诱导培养的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年06期页码: 103-105 栏目: 出版日期: 2012-06-16

Title:: A Study on Calli Induction and Culture from Daucus carota L.

文章编号:: 1001-0009（2012）06-0103-03

作者:: 汪洪¹; 2; 夏鸿亮²; 李校堃²; 杨树林¹; 1.南京理工大学环境与生物工程学院，江苏南京 210094；
2.温州医学院药学院，浙江温州 325035

Author(s):: WANG Hong¹; 2; XIA Hong-liang²; LI Xiao-kun²; YANG Shu-lin¹; 1.School of Environmental and Biological Engineering，Nanjing University of Science and Technology，Nanjing,Jiangsu 210094;
2.School of Pharmacy，Wenzhou Medical College,Wenzhou,Zhejiang 325035

关键词:: 胡萝卜; 愈伤组织; 组织培养

Keywords:: carrot; calli; tissue culture

分类号:: S 631.203.6

文献标志码:: A

摘要:: 以胡萝卜“七寸人参”为试材，探讨了不同外植体、激素、培养基、光照对胡萝卜愈伤组织诱导的影响。结果表明：胡萝卜愈伤组织的诱导以下胚轴为外植体为宜；在添加2,4-D 1.0 mg/L和KT 0.5 mg/L的MS培养基上愈伤组织的诱导频率为100%，且愈伤组织生长良好，增殖快速；光照对愈伤组织的诱导没有影响，但却进一步阻碍了愈伤组织的增殖生长。

Abstract:: Calli induction and proliferation from explants were regulated by several factors,the effect of hormones，different explants，light and media on calli induction from Daucus carota L.was investigated.The results indicated that the optimal explant for calli induction from carrot was hypocotyl rather than cotyledon.The optimal medium for calli induction was MS agar medium supplemented with 3% sucrose,2,4-D 1.0 mg/L and KT 0.5 mg/L.The induced calli frequency of the carrot hypocotyls on this medium was 100%.Light had no effect on calli induction but it influenced calli proliferation.

参考文献/References:

[1]Luchakivskaya Y,Kishchenko O,Gerasymenko I,et al.High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.)[J].Plant Cell Rep，2011,30:407-415.

[2]Imani J,Lorenz H,Kogel K H,et al.Transgenic Carrots：Potential Source of Edible Vaccines [J].J.Verbr.Lebensm，2007,2(Supplement1):105.

[3]Sunil Kumar G B,Ganapathi T R,Bapat V A.Production of Hepatitis B Surface Antigen in Recombinant Plant Systems：An Update[J].Biotechnol Prog，2007,23:532-539.

[4]Shaaltiel Y,Bartfeld D,Hashmueli S,et al.Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement therapy of Gaucher’s disease using a plant cell system [J].Plant Biotechnol J,2007(5):579-590.

[5]周玲艳,秦华明.胡萝卜悬浮细胞系及高效再生系统的建立[J].安徽农业科学，2006,34(16):3929-3931.

[6]杨艳梅,刘玲,巩振辉,等.胡萝卜高效再生体系的建立[J].华北农学报,2004,19(1):10-12.

[7]高金秋,于丽杰.胡萝卜体细胞胚再生系统的建立[J].北方园艺,2009(8):73-77.

[8]褚四敏,陈敏洁,贾文姝,等.光质对植物组织培养的影响[J].安徽农业科学,2011,39(2)：665-666,672.

[9]周玲艳,秦华明,梁红,等.胡萝卜再生体系的建立[J].农业与技术,2005,25(3):94-95.

[10]张娅,曾君祉,周志勇,等.胡萝卜组织培养和高效遗传转化体系的建立[J].植物学通报，2005,22（增刊）:37-42.

[11]Franklin G,Dias A C.Chlorogenic acid participates in the regulation of shoot，root and root hair development in Hypericum perforatum[J].Plant Physiol Biochem,2011,49(8):835-842.

[12]Sato S,Toya T,Kawahara R,et al.Isolation of a carrot gene expressed specifically during early-stage somatic embryogenesis[J].Plant Molecular Biology,1995,28(1):39-46.

[13]Kitamiya E,Suzuki S,Sano T,et al.Isolation of two genes that were induced upon the initiation of somatic embryogenesis on carrot hypocotyls by high concentrations of 2,4-D [J].Plant Cell Reports，2000,19:551-557.

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备注/Memo

第一作者简介：汪洪（1974-）,女,博士，副教授，现主要从事植物生物技术方面的研究工作。E-mail：anghong@126.com。

责任作者：杨树林（1953-），男，教授，博士生导师，现主要从事生物技术方面的研究工作。E-mail：yshulin@mail.njust.edu.cn。

基金项目：浙江省自然科学基金资助项目（Y3100459）。收稿日期：2011-10-17

更新日期/Last Update: 2014-08-30