LUO Jun-qin,XU Jian-guo,WANG Ping.Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus[J].Northern Horticulture,2013,37(13):125-127.
不同培养条件对柑桔胚性愈伤组织离体诱导的影响
- Title:
- Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus
- 文章编号:
- 1001-0009(2013)13-0125-03
- Keywords:
- citrus; callus; induction; influence factors
- 分类号:
- S 6661
- 文献标志码:
- A
- 摘要:
- 以5个不同品种柑桔为试材，研究比较了不同培养基激素浓度、接种外植体类型及不同品种实生试管苗下胚轴对柑桔离体培养外植体愈伤及胚性愈伤诱导分化的影响。结果表明：下胚轴外植体胚性愈伤诱导效果以BA1.0 mg/L+NAA 0.5 mg/L为好，而子叶外植体则以BA 2.0 mg/L+NAA 1.0 mg/L或更高激素浓度条件为宜；外植体胚性愈伤诱导能力以下胚轴最强，子叶与幼根外植体次之，上胚轴最弱。5个供试柑桔品种下胚轴胚性愈伤诱导能力“本地早蜜桔”最强，其次是“四季桔”、“南丰蜜桔”，再次为“刘本橙”，“罗浮金弹”最弱。
- Abstract:
- Taking 5 different varieties of citrus as materials，the effect of different hormone concentrations，explants and varieties on embryogenic callus induction of citrus in vitro were studied.The results showed that the optimal hormone concentration media for inducing embryogenic callus from hypocotyls was MT+BA 1.0 mg/L+NAA 0.5 mg/L，while that for cotyledons was MT+BA 2.0 mg/L+NAA 1.0 mg/L or much higher;the ability of forming embryogenic callus from explants were hypocotyls>cotyledons，rootlets>epicotyls;and the ability of induced embryogenic callus from hypocotyls for citrus varieties were ‘Bendizao mandarin’>‘Calamondin’，‘Nanfeng mandarin’>‘Luiben orange’>‘Oval kumquat’.
参考文献/References:
[1]张俊娥,郭文武,邓秀新.柑橘愈伤组织倍性变化及其与体细胞胚胎发生能力的关系[J].遗传学报(英文版),2006,33(7):647-654.
相似文献/References:
[1]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(13):108.
[2]闫海霞,黄昌艳,何荆洲,等.观赏凤梨组织培养的影响因素分析[J].北方园艺,2013,37(22):196.
YAN Hai-xia,HUANG Chang-yan,HE Jing-zhou,et al.Analysis of the Influence Factors in Tissue Culture of Ornamental Bromeliads[J].Northern Horticulture,2013,37(13):196.
[3]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(13):91.
[4]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(13):108.
[5]马少梅.北海道黄杨再生体系建立研究进展[J].北方园艺,2013,37(13):208.
MA Shao-mei.Research Advances on the Establishment of Regeneration System of Euonymus japonicus[J].Northern Horticulture,2013,37(13):208.
[6]于 娜,董宽虎.白羊草成熟种子组织培养再生体系研究[J].北方园艺,2014,38(02):104.
YU Na,DONG Kuan-hu.Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum [J].Northern Horticulture,2014,38(13):104.
[7]张丽霞,王毅彰.植物生长调节剂对花籽山药愈伤组织诱导形成的影响[J].北方园艺,2013,37(14):115.
ZHANG Li-xiaWANG Yi-zhang.Effect of Plant Growth Substances on Callus Induction of Seed Yam Forming[J].Northern Horticulture,2013,37(13):115.
[8]李金英,赵春莉,张馥茜,等.黑穗醋栗愈伤组织诱导研究[J].北方园艺,2013,37(17):87.
LI Jin-ying,ZHAO Chun-li,ZHANG Fu-qian,et al.Study on the Callus Induction of Blackcurrant[J].Northern Horticulture,2013,37(13):87.
[9]于艳萍,韩善峰,赵登超,等.嫩枝扦插不同处理对“香玲”核桃组织再生能力的影响[J].北方园艺,2013,37(17):91.
YU Yan-ping,HAN Shan-feng,ZHAO Deng-chao,et al.Effect of Different Softwood Cutting Treatments on the Tissue Regenerating Ability of Juglans regia ’Xiangling’[J].Northern Horticulture,2013,37(13):91.
[10]苗晓燕,何富强,张筱梅.北沙参愈伤组织诱导及悬浮细胞培养研究[J].北方园艺,2014,38(06):104.
MIAO Xiao-yan,HE Fu-qiang,ZHANG Xiao-mei.Study on Initiation of Callus Tissues and Suspension Cell Culture of Glehnia littoralis[J].Northern Horticulture,2014,38(13):104.
备注/Memo
第一作者简介：罗君琴（1972-），女，硕士，助理研究员，现主要从事园艺植物组织培养等研究工作。E-mail:english0576@163.com.