HE Liming,CAI Ling,XIE Xin,et al.Isolation and Identification of Pathogen of Trichoderma Ganoderma and Screening of Resistant Germplasm[J].Northern Horticulture,2025,(2):59-66.[doi:10.11937/bfyy.20243360]
灵芝木霉病病原菌分离鉴定及抗病种质筛选
- Title:
- Isolation and Identification of Pathogen of Trichoderma Ganoderma and Screening of Resistant Germplasm
- 文章编号:
- 1001-0009(2025)02-0059-08
- Keywords:
- Ganoderma lucidum; Trichoderma; resistance screening
- 分类号:
- S 567.3+1
- 文献标志码:
- A
- 摘要:
- 以染病灵芝菌袋和子实体为试材，采用组织分离纯化法、ITS序列测序、平板对峙、菌袋筛选等方法，研究了灵芝木霉病病原菌的分类地位及抗病种质筛选，以期为木霉病的绿色防治提供参考依据。结果表明：4个木霉菌株被鉴定为木霉属的3个种，D14和JY2为哈茨木霉（Trichoderma harzianum），JY1为侧耳木霉（T.pleuroticola），Lv为长枝木霉（T.longibrachiatum）。4个木霉菌株在平板上和袋料中的侵染性强弱表现基本一致，从种类上侵染性强弱表现为侧耳木霉>哈茨木霉>长枝木霉。不同灵芝菌株与4个木霉菌株的抗性情况明显不同。该研究成功筛选获得1个抗木霉病的优质灵芝菌株ZL18，为高抗性优质灵芝新品种的选育和木霉病的有效防治奠定基础。
- Abstract:
- Ganoderma lucidum bag and fruiting body were used as the experimental materials.The taxonomic status of pathogen of Trichoderma Ganoderma and the screening of diseaseresistant strains were studied by using the tissue isolation and purification technology method，ITS sequencing and plate confrontation，bag screening，in order to provide reference for the green control of Trichoderma.The results showed that the four strains were identified as three species of Trichoderma,D14 and JY2 as Trichoderma harzianum，JY1 as T.pleuroticola，and Lv as T.longibrachiatum.The infectivity of the four Trichoderma strains on the agar plate and in the bag was basically the same，and the infectivity in terms of species was as follows,T.pleuroticola>T.harzianum>T.longibrachiatum.There were significant differences among G.lucidum strains in resistance to four Trichoderma strains.A highquality G.lucidum strain ZL18 resistant to Trichoderma was screened，laying the foundation for the breeding of new high resistant and highquality G.lucidum variety and effective prevention and control of trichoderma ganoderma.
参考文献/References:
［1］国家药典委员会．中华人民共和国药（一部）［M］．2020年版.北京：中国医药科技出版社，2020.［2］CHEN T Q，WU J G，KAN Y J，et al．Antioxidant and hepatoprotective activities of crude polysaccharide extracts from Lingzhi or reishi medicinal mushroom，Ganoderma lucidum (Agaricomycetes)，by ultrasonic-circulating extraction［J］．International Journal of Medicinal Mushrooms，2018，20(6)：581-593．［3］WASSER S P．Medicinal mushrooms in human clinical studies．part I．anticancer，oncoimmunological，and immunomodulatory activities：A review［J］．International Journal of Medicinal Mushrooms，2017，19(4)：279-317．［4］DAN X，LIU W，WONG J H，et al．A ribonuclease isolated from wild Ganoderma lucidum suppressed autophagy and triggered apoptosis in colorectal cancer cells［J］．Frontiers in Pharmacology，2016（7）：217．［5］解宜林,檀根甲.灵芝绿霉病发病原因分析及防治措施［J］.中国食用菌,2015,34(1):74-76.［6］黄鑫伟,严寅珲,张通,等.灵芝绿霉病病原菌的分离鉴定及快速检测［J］.植物保护学报,2018,45(6):1435-1436.［7］李承达,张亚鑫,刘权德,等.长枝木霉引发灵芝绿霉病的快速检测体系的建立［J］.天津农业科学,2021,27(7):61-65.［8］林汝楷,刘叶高,高珠清.灵芝哈茨木霉病杀菌剂筛选试验［J］.食用菌,2008(1)：55-56.［9］解鹏飞,郭建峰,张春兰,等.灵芝哈茨木霉病害生防细菌的筛选及菌剂制备研究［J］.微生物学杂志，2018，38(6)：75-83.［10］LU B H，ZUO B，LIU X L，et al．Trichoderma harzianum causing green mold disease on cultivated Ganoderma lucidum in Jilin province，China［J］．Plant Disease，2016，100(12)：2524．［11］刘正慧，李丹，SOSSAH F L，等．食用菌主要病原真菌和细菌［J］．菌物研究，2018，16(3)：158-163．［12］王洁，梁丽婷，谢欣怡，等．一株肉桂炭疽病病原菌分离鉴定及生防菌筛选［J］．北方园艺，2024(8)：102-108．［13］吴小平．食用菌致病木霉的鉴定、致病机理及防治研究［D］．福州：福建农林大学，2008．［14］周莉．平菇单孢杂交及抗病新菌株选育［D］．南宁：广西大学，2014．［15］金鑫,刘宗敏,黄羽佳,等.我国灵芝栽培现状及发展趋势［J］.食药用菌,2016,24(1):33-37.［16］曾华兰.哈茨木霉T23及其防病作用研究［D］.雅安:四川农业大学，2005.［17］张宏浩，王亚磊，冀瑞卿．几种食用菌的抗病性分析［J］．北方园艺，2015(17)：127-129．［18］熊小龙．四川金针菇木霉病害的研究及金针菇菌株抗性分析［D］．雅安：四川农业大学，2011．［19］李冠霖，徐洋，邢鹏杰，等．木霉对不同香菇菌丝体生长阶段的致病性及生防制剂对其的抑制效果［J］．分子植物育种，2019，17(19)：6530-6534．［20］江晴．香菇种质资源群体抗木霉能力分化的初步研究［D］．武汉：华中农业大学，2017．
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备注/Memo
第一作者简介：贺黎铭（1988-），女，硕士，副研究员，现主要从事菌类药材育种与栽培等研究工作。Email：h121260@126.com.责任作者：罗霞（1974-），女，博士，研究员，现主要从事菌类药材研究与开发等研究工作。Email：287748567@qq.com.基金项目：四川省省级科研院所基本科研业务费资助项目（2023JDKY0027）；成都市科技资助项目（2024YF0502175SN,2019YF0502432SN）；四川省科技计划资助项目（2021YFYZ0012）；国家现代农业产业技术体系四川道地中药材创新团队资助项目（SCCXTD202019）；四川省“天府万人计划”资助项目（川万人第407号）。收稿日期：2024-08-21