HOU Yanxia,TANG Haoru,LIN Yuanxiu,et al.Cloning and Expression of Faapx-c Gene and Bioinformatics Analysis in Fragaria×ananassa Based on RACE[J].Northern Horticulture,2016,40(11):88-92.[doi:10.11937/bfyy.201611023]
基于RACE方法的草莓Faapx-c基因克隆、表达及其生物信息学分析
- Title:
- Cloning and Expression of Faapx-c Gene and Bioinformatics Analysis in Fragaria×ananassa Based on RACE
- Keywords:
- Fragaria×ananassa; Faapx-c gene; RACE; cloning; expression; bioinformatics
- 文献标志码:
- A
- 摘要:
- 以草莓栽培品种“丰香”为试材，利用RT-PCR和3′-RACE技术克隆胞质抗坏血酸过氧化物酶基因Faapx-c，并对其进行了生物信息学分析。结果表明：Faapx-c基因长1 034 bp，所编码的蛋白FaAPX-c是一个结构稳定、亲水性、非分泌型的蛋白质，其二级结构主要以α-螺旋和无规则卷曲2种形式出现，具有可靠的三维结构，属于植物过氧化物酶家族。Faapx-c基因在草莓的根、茎和叶中能被低温诱导，表达量先升高后下降，而在花中的表达随低温时间的延长而消失。该研究为进一步进行Faapx-c基因的功能研究奠定了基础。
- Abstract:
- Taking Fragaria×ananassa cv.Toyonaka as material，a cytosolic ascorbate peroxidase gene Faapx-c was cloned by RT-PCR and 3′-RACE technology，and the bioinformatic analysis of Faapx-c was carried out.The results showed that Faapx-c was 1 034 bp in length coding a protein FaAPX-c，which was a stable，hydrophilicity and non-secreted protein.The secondary structure of FaAPX-c contained mainly α-helix and random coil，with reliable 3D structure.And the protein belonging to a member of plant peroxidase superfamily.Expression analysis of Faapx-c gene showed that it was induced by low temperature in roots，stems，leafs，and the expression amounts were increased firstly and then decreased,but gradually disappeared in flowers.This study laid the foundation for further research on the function of Faapx-c gene.
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备注/Memo
第一作者简介：侯艳霞（1981-），女，博士，讲师，研究方向为园艺植物生物技术和遗传育种。E-mail：yxhou2007@163.com.责任作者：汤浩茹（1963-），男，博士，教授，研究方向为园艺植物生物技术和遗传育种。E-mail：htang@sicau.edu.cn.基金项目：山西省基础研究资助项目（2013021026-2）；山西林业职业技术学院院级科研教改资助项目(201314)。