[1]刘万达,张丙秀,魏媛媛,等.草莓DHAR基因密码子偏好性分析[J].北方园艺,2014,38(14):92-97.
　LIU Wan-da,ZHANG Bing-xiu,WEI Yuan-yuan,et al.Analysis of Codon Bias of DHAR Gene in Strawberry[J].Northern Horticulture,2014,38(14):92-97.
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草莓DHAR基因密码子偏好性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年14期 页码: 92-97 栏目: 出版日期: 2014-07-30
Title:
Analysis of Codon Bias of DHAR Gene in Strawberry
文章编号:
1001-0009（2014）14-0092-06
作者:
刘万达1; 张丙秀2; 魏媛媛2; 王 禹1; 董 超2; 朱延明3
1.黑龙江省农业科学院 园艺分院，黑龙江 哈尔滨 150069；
2.东北农业大学 园艺学院，黑龙江 哈尔滨 150030;
3.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030
Author(s):
LIU Wan-da1; ZHANG Bing-xiu2; WEI Yuan-yuan2; WANG Yu1; DONG Chao2; ZHU Yan-ming3
1.Horticultural Sub-Academy,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150069;
2.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030;
3.College of Life Science,Northeast Agricultural University,Harbin，Heilongjiang 150030
关键词:
草莓; DHAR; 密码子偏好性; 聚类分析; 基因表达
Keywords:
strawberry; DHAR; codon bias; cluster analysis; gene expression
分类号:
S 668.4
文献标志码:
A
摘要:
以草莓为试材，运用CHIPS、CUSP和CodonW程序分析了草莓DHAR基因的密码子偏好性，并与大肠杆菌和酵母的基因组密码子偏好性及7种植物的DHAR基因密码子偏好性进行了比较，同时基于DHAR基因的密码子使用偏好性进行了系统聚类分析，以期在作物遗传改良中为草莓DHAR基因选择合适的遗传转化受体及进行基因优化提供依据。结果表明：草莓DHAR基因更适合导入苹果等双子叶植物中，若要提高草莓DHAR基因在大肠杆菌或酵母中的表达水平，还需进行密码子的优化。
Abstract:
Taking strawberry as materials,CHIPS,CUSP and CodonW were used to analyze the codon bias of DHAR gene in strawberry,in order to select appropriate transformation receptor for strawberry ?DHAR??gene in crop genetic improvement and provide reference for genetic optimization.The codon bias of E.coli and yeast genome,codon bias of?DHAR??gene in 7 kinds of plants and strawberry were compared,and based on codon bias of?DHAR??gene the cluster analysis was made.The results showed that the strawberry?DHAR?gene was more suitable for introduction to apple or other dicotyledonous plants;to improve the expression level of strawberryDHAR??gene in?E.colior yeast,codon optimization was required.

参考文献/References:

[1]陈坤明，宫海军，王锁民，植物抗坏血酸的生物合成、转运及其生物学功能[J].西北植物学报，2004,24(2)：329-336.
[2]刘永立，胡海涛，兰大伟．维生素C的生物合成及其基因调控研究进展[J].果树学报，2006,23(3)：431-436.
[3] Yabuta Y,Motoki T,Yoshimura K, et al.Thylakoid membrane-bound ascorbate peroxidase is a limiting factor of antioxi-dative systems under photo-oxidative stres[J]. Planta, 2002, 32: 915-925.
[4]石秀凡，黄京飞，柳树群，等．人类基因同义密码子偏好的特征以及与基因GC含量的关系[J].生物化学与生物物理进展，2002, 29 (3)：411-414.
[5] Urrutia A 0,Hurst L n Codon usage bias covaries with expressionbreadth and the rate of synonymous evolution in humans, but this is notevidence for selection[J]. Genetics, 2001,159 (3):1191-1199.
[6] Sueoka N.Kawanishi Y. DNA G+C content of the third codon position and codon usage biases of human genes[J]. Gene, 2000, 261(1): 53-62.
[7] Sharp P M,Li W H An evolutionary perspective on synonymous codon usage in unicellular organisms[J].J Mol Ev01,1986, 24(1-2): 28-38.
[8] Zhou H,Wang H, HuangL F,et al.Heterogeneity in codon usages of sobemovirus genes[J]. Arch Virol, 2005 ,150(8): 1591-1605.
[9] Sharp P M.Cowe E, Higgins D G,et al. Codon usage patter ns in Esch-erichia coli,Bacillus subtilis,Saccharomvces cere uisiae,Schizosaccharomyces pombe,DrosophiLa melanogaster and Homo sapiens:A review of the considerable within species diversity[J]. Nucl Acids Res,1988 ,16 (17): 8207-8211.
[10]孪平，白云凤，冯瑞云，等．籽粒苋苹果酸酶(NADME)基因密码子偏好性分析[J]．应用与环境生物学报，2011,17(1)：12-17.

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备注/Memo

第一作者简介：刘万达（1982-），男，黑龙江富裕人，硕士，助理研究员，现主要从事寒地果树新品种选育与配套栽培技术的研究及示范推广工作。E-mail：haaslwd@126.com.

责任作者：朱延明（1955-），男，博士，教授，现主要从事作物耐盐碱与低温分子生物学及基因工程等教学与科研工作。E-mail：ymzhu2001@yahoo.com.cn.
基金项目：农业生物功能基因重点实验室开放课题资助项目(NSGJ2012-05)。
收稿日期：2014-03-21

更新日期/Last Update: 2014-08-07