ZHANG Tingting,LUO Shuangxia,WANG Xing,et al.Technology Research on Reserving Descendants From Distant Hybridization of Eggplant by Micro-cutting[J].Northern Horticulture,2016,40(11):92-95.[doi:10.11937/bfyy.201611024]
微扦插法保存茄子远缘杂交后代技术研究
- Title:
- Technology Research on Reserving Descendants From Distant Hybridization of Eggplant by Micro-cutting
- Keywords:
- eggplant; micro-cutting; distant hybridization descendant; reserve
- 文献标志码:
- A
- 摘要:
- 以茄子远缘杂交F2代群体为试材，研究了不同杀菌剂、处理时间和培养基成分对接种茎段及其腋芽生长情况的影响。结果表明：酒精处理30 s后再用0.1%升汞处理7~8 min，茎段杀菌效果最好，健康茎段率为40%~55%；酒精处理30 s后再用0.1%升汞处理2~3 min，新芽杀菌效果最佳，新芽健康可用率最高为73.33%；适宜的新芽增殖培养基为MS+0.02 mg?L-1 NAA+1.0 mg?L-1 6-BA。
- Abstract:
- Taking the F2 descendants from distant hybridization of eggplant as material,the effects of different sterilants and treatment time，and medium components on the growth of stem segments and axillary buds were studied.The result showed that the rate of healthy stem segments was 40%-55% after sterilizing with alcohol for 30 seconds and then 0.1% HgCl2 for 7-8 minutes；the method to sterilize eggplant axillary buds with alcohol for 30 seconds and then 0.1% HgCl2 for 2-3 minutes was effective and the highest rate of the axillary buds was 73.33%；the optimum multiplication medium for axillary buds was MS supplemented with 0.02 mg?L-1 NAA and 1.0 mg?L-1 6-BA.
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备注/Memo
第一作者简介：张婷婷（1990-），女，硕士研究生，研究方向为蔬菜育种。E-mail:472351822@qq.com.责任作者:陈雪平（1971-），男，博士，教授，研究方向为蔬菜遗传育种。E-mail:chenxueping@hebau.edu.cn.基金项目：“十二五”农村领域国家科技计划资助项目（2012BAD02B02-3）；河北省现代农业产业技术体系蔬菜创新团队资助项目。