XU Delin,SHEN Fang,QIAN Gang,et al.Effect of Different Hormone Combinations on the Induction,Proliferation and Differentiation of Callus in Bletilla striata[J].Northern Horticulture,2016,40(12):157-161.[doi:10.11937/bfyy.201612039]
不同激素配比对白及愈伤组织诱导增殖和分化的影响
- Title:
- Effect of Different Hormone Combinations on the Induction,Proliferation and Differentiation of Callus in Bletilla striata
- Keywords:
- Bletilla striata; callus; induction; proliferation; differentiation; medium
- 文献标志码:
- A
- 摘要:
- 以白及为试材，以MS和1/2MS为基本培养基，研究了愈伤组织诱导、增殖和分化培养基中的最佳激素种类和浓度配比。结果表明：在MS中添加6-BA 1 mg?L-1和2,4-D 3 mg?L-1时白及愈伤的诱导率最高，达89.89%。2,4-D是诱导白及愈伤生成的关键激素，而在基本培养基中单添加6-BA时并不能诱导愈伤组织的生成，同时添加2,4-D和6-BA能有效促进愈伤的诱导，且愈伤诱导率随着2,4-D浓度的增加而逐渐增加，但优质愈伤率也随之降低。添加6-BA 0.1 mg?L-1和2,4-D 2 mg?L-1的增殖培养基中愈伤细胞的增殖率最高，30 d的增殖倍数达7.457。在分化培养基中添加NAA和TDZ有利于白及愈伤组织分化，分化率随着二者浓度的增加而增加，且NAA的促分化效果优于TDZ。MS+2,4-D 2 mg?L-1+NAA 1 mg?L-1为最佳分化培养基，分化30 d时其分化率为86.67%。
- Abstract:
- Bletilla striata(Thunb.)Rchb.f.was used in this study to screen the optimal mediums in callus induction,proliferation and differentiation through the comparisons of different hormones combined with the concentrations based on the medium of MS and 1/2MS.The results showed that the highest inducing rate was observed as 89.89% at the combination of MS+6-BA 1 mg?L-1+2,4-D 3 mg?L-1.6-BA(6-Benzylaminopurine)alone in the basic medium exhibited disability in producing the callus,while this disability was determined by adding 2,4-D(2,4-D ichlorophenoxyacetic acid)simultaneously.And the inducing rate increased as more 2,4-D was added,while the products quality decreased as the increasing of 2,4-D in the basic medium.Additionally,the combination of MS+6-BA 0.1 mg?L-1+2,4-D 2 mg?L-1 exhibited the highest proliferation rate(745.7%)than the other combinations after 30 days incubation.Increasing callus differentiation rate was observed as the increasing of NAA(alpha-Naphthylacetic acid)and TDZ(thidiazuron)in the basic medium,while NAA alone possessed better effect than TDZ.And the combination of MS+2,4-D 2 mg?L-1+NAA 1 mg?L-1 exhibited the highest differentiation rate(86.67%)than other combinations after 30 days differentiation.
参考文献/References:
[1]SINGH A,DUGGAL S.Medicinal orchids-an overview[J].Ethnobotanical Leaflets,2009(13):399-412. [2]ZHONG Y.Integrative and discovery of conventional drugs[M]//ALAOUI-JAMALI M.Alternative and complementary therapies for cancer.New York:Springer US,2010:105-133. [3]JIANG F S,LI W P,HUANG Y F,et al.Antioxidant,antityrosinase and antitumor activity comparison:The potential utilization of fibrous root part of Bletilla striata(Thunb.)Reichb.f.[J].PLoS One,2013,8(2):e58004. [4]DIAO H,LI X,CHEN J,et al.Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages[J].Journal of Bioscience and Bioengineering,2008,105(2):85-89. [5]赵艳霞,邓雁如,张晓静,等.白及属药用植物化学成分及药理作用研究进展[J].天然产物研究与开发,2013,25(8):1137-1145. [6]陆峻波,刘亚辉,杨永红,等.从文献分析看我国白芨研究进展[J].云南农业大学学报,2011,26(2):288-292. [7]林伊利,李伟平,马丹丹,等.白及组织快繁的实验研究[J].中华中医药学刊,2012,30(2):336-339. [8]韦卡娅,刘燕琴,秦静,等.白及组培外植体的筛选研究[J].中国现代中药,2008,10(5):13-14. [9]王跃华,段茂华,任三军,等.山葵愈伤组织的诱导与增殖研究[J].江西农业科学,2015,43(1):53-54. [10]石云平,赵志国,唐凤鸾,等.白芨愈伤组织诱导、增殖与分化研究[J].中草药,2013,44(3):349-353. [11]王镨,宋家祥,付群英.药用植物决明的愈伤组织诱导与生长增殖的培养条件研究[J].中草药,1997,12(12):739-742.
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备注/Memo
第一作者简介：徐德林（1981-），男，博士，副教授，研究方向为中药材遗传育种。E-mail:xudelin2000@163.com.基金项目：国家自然科学基金资助项目（31560079）；贵州省中药现代化科技产业研究开发专项资助项目（黔科合ZY字[2013]3002号）；贵州省科技厅、遵义医学院、遵义市科技局联合基金重点资助项目（黔科合LH字[2014]7549号）；贵州省教育厅2013年大学生创新创业训练计划资助项目（201410661019）；遵义医学院2013年大学生创新资助项目（院发[2013]6516）。