[1]李林,上官艳妮,黄恻隐,等.白及愈伤组织与内生真菌共培养及次生代谢产物含量变化[J].北方园艺,2021,(14):128-134.[doi:10.11937/bfyy.20203320]
　LI Lin,SHANGGUAN Yanni,HUANG Ceyin,et al.Co-culture System of Bletilla striata Callus With Endophytic Fungi and Changing Contents of Secondary Metabolites[J].Northern Horticulture,2021,(14):128-134.[doi:10.11937/bfyy.20203320]
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白及愈伤组织与内生真菌共培养及次生代谢产物含量变化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年14 页码: 128-134 栏目: 出版日期: 2021-07-30
Title:
Co-culture System of Bletilla striata Callus With Endophytic Fungi and Changing Contents of Secondary Metabolites
作者:
李林1; 上官艳妮1; 黄恻隐1; 吴明开2; 徐玲玲3; 徐德林1
（1.遵义医科大学 基础医学院，贵州 遵义 563000；2.贵州省农作物品种资源研究所，贵州 贵阳 550006；3.西安文理学院 生物与环境工程学院,植物与微生物协同研究重点实验室，陕西 西安 710065）
Author(s):
LI Lin1; SHANGGUAN Yanni1; HUANG Ceyin1; WU Mingkai2; XU Lingling3; XU Delin1
(1.School of Basic Medicine Sciences,Zunyi Medical University,Zunyi,Guizhou 563000;2.Institute of Modern Chinese Herbal of Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006;3.School of Biological and Environmental Engineering,Xi′an University/Key Laboratory of Plant-microbe Collaboration Research,Xi′an,Shaanxi 710065)
关键词:
白及; 愈伤组织; 内生真菌; 次生代谢产物; 共培养
Keywords:
Bletilla striata; callus; endophytic fungi; secondary metabolites; co-culture
DOI:
10.11937/bfyy.20203320
文献标志码:
A
摘要:
以白及愈伤组织为试材，采用与内生真菌共培养的方法，研究了该过程中白及愈伤组织、内生菌生长速率及4种主要次生代谢含量的变化，以期为白及内生菌在药用成分的开发利用提供参考依据。结果表明：7株受试真菌菌株与白及愈伤组织分别共培养3、6 d时均表现出高于真菌单培养形成的菌面积，共培养9 d时各组愈伤增值率显著低于对照（CK）组；1株受试内生真菌生长缓慢，在共培养与单独培养3、6、9 d均具有相近菌面积。进一步对8株内生真菌共培养处理下的白及次生代谢产物含量检测发现，不同内生真菌对dactylorhin A、1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯、4-甲氧基-9,10-二氢菲-2,7-二醇、对羟基苯甲醇的积累趋势具有不同影响，主要呈现出3种累积趋势。
Abstract:
The callus of Bletilla striata were used as material,co-cultured with endophytic fungi method was used to study the growth rate of callus with endophyte,and contents changing of four main secondary metabolites in the period,in order to provide a reference for the development and utilization of endophytes in Bletilla striata.The results showed that the areas of 7 fungal strains co-cultured with callus of Bletilla striata for 3 days and 6 days were higher than that of endophyte single culture,and the callus proliferation rate of callus of each treatment group was significantly lower than that of control (CK) group for 9 days.And one of the tested endophytic fungi grew slowly,and displayed almost the same fungi area in co-culture treatment with culture alone for 3,6,9 days.Furthermore,the secondary metabolism contents of callus in Bletilla striata co-cultured with 8 endophytic fungi,showed that different endophytic fungi had various effects on the accumulation trend of dactylorhin A,4-Hydroxybenzaldehyde,militarine,and coelonin.

参考文献/References:

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备注/Memo

第一作者简介：李林（1982-），女，博士，副教授，现主要从事中药材遗传育种等研究工作。E-mail:lilin@zmu.edu.cn.责任作者：徐德林（1981-），男，博士，教授，现主要从事中药材细胞工程等研究工作。E-mail:xudelin2000@163.com.基金项目：国家自然科学基金资助项目（31560079,31560102,31960074）；贵州省科学技术基金资助项目（黔科合平台人才[2017]5733-050,黔科合平台人才[2019]5657,黔科合平台人才[2017]5712）；贵州省科技厅人才成长资助项目（KY[2017]194）。收稿日期：2020-08-06

更新日期/Last Update: 2021-11-13