YUAN Wan-jun,YANG Ling,FU Rong,et al.Study on Tissue Culture of Rosa ‘Aglaia’[J].Northern Horticulture,2015,39(07):107-109.[doi:10.11937/bfyy.201507031]
藤本月季‘安吉拉’组织培养研究
- Title:
- Study on Tissue Culture of Rosa ‘Aglaia’
- Keywords:
- Rosa ‘Aglaia’; tissue culture; growth regulator
- 文献标志码:
- A
- 摘要:
- 以藤本月季‘安吉拉’的茎段为外植体，进行组织培养快速繁殖技术研究，以期筛选出适合的消毒方法和培养基配方。结果表明：0.1%的升汞浸泡外植体9 min消毒效果最佳；培养基MS+6-BA 2.0 mg/L腋芽萌发率高达100%，腋芽成芽率94.74%；培养基MS+6-BA 0.5 mg/L+NAA 0.10 mg/L为比较适合的腋芽增殖培养基，增殖系数最高，且增殖芽生长健壮。
- Abstract:
- Using Rosa ‘Aglaia’ stem segments as explants，the tissue culture and rapid propagation were studied，in order to optimize and screen the appropriate disinfection methods and suitable medium.The results showed that explants soaking in 0.1% mercuric chloride 9 minutes，disinfection effect was the best.The axillary bud germination rate was as high as 100% and bud formation rate was 94.74% in the medium MS+6-BA 2.0 mg/L；the best medium for multiplication of axillary bud was MS+6-BA 0.5 mg/L+NAA 0.10 mg/L，not only the highest multiplication coefficient，but also having health and strong axillary buds.
参考文献/References:
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备注/Memo
第一作者简介：袁婉君(1990-)，女，云南昆明人，硕士研究生，研究方向为园林植物与观赏园艺。E-mail：258735141@qq.com. 责任作者：王锦(1966-)，女，北京人，博士，教授，博士生导师，现主要从事园林植物与观赏园艺等研究工作。E-mail：908505685@qq.com. 基金项目：国家林业局“948”资助项目(2014-4-19)；云南省高校林下生物资源保护及利用科技创新团队资助项目。