ZHANG Yongping,QIAO Yongxu,ZHENG Ping,et al.Tissue Culture and Rapid Propagation System and Estimation of Annual Yield of Group Seedlings of Anthurium addaeanum ‘Black Queen’[J].Northern Horticulture,2023,(24):65-71.[doi:10.11937/bfyy.20231644]
红掌‘黑皇后’无性快繁体系的建立及组培苗年产量估算
- Title:
- Tissue Culture and Rapid Propagation System and Estimation of Annual Yield of Group Seedlings of Anthurium addaeanum ‘Black Queen’
- 文章编号:
- 1001-0009(2023)24-0065-07
- Keywords:
- Anthurium andraeanum; culture in vitro; callus tissue; regenerating mass; rapid propagation
- 分类号:
- S 682
- 文献标志码:
- A
- 摘要:
- 以红掌新品种“黑皇后”植株的嫩叶、叶柄和根段为外植体，对影响愈伤组织诱导、愈伤组织继代和不定芽分化、再生团块继代和不定芽分化、不定芽生根等因素进行研究，同时采用数学公式推算组培苗的年产量,以期为红掌适度规模化繁育和栽培提供参考依据。结果表明：愈伤组织诱导的最佳外植体是叶片,其次为叶柄,最后为根段，诱导率分别为70%、40%、35%；愈伤组织诱导的最佳培养基为1/2MS+6-BA0.5+2,4-D0.5；愈伤组织继代和不定芽分化的最佳培养基为MS+6-BA1.0+2,4-D0.2；再生团块继代和不定芽分化的最佳培养基为MS+6-BA1.0+NAA0.2；丛生芽生根的最佳培养基为1/2MS+NAA0.2；以芽、嫩茎和根段直接生芽的模式，1瓶原种年产苗量Y=mXn；以再生团块或愈伤组织生芽的模式，1瓶原种年产苗量Y=ac(1-Xn)/(1-X)。
- Abstract:
- The factors affecting the induction of callus tissue，succession of callus tissue and differentiation of adventitious buds，succession of regenerating mass and of adventitious buds，and rooting of adventitious buds were investigated by using young leaves，petioles and root segments of the new variety ‘Black Queen’ as explants，and the annual yield of the seedlings was calculated by using mathematical formula,in order to provide reference for A.andraeanum moderate scale breeding and cultivation.The results showed that the best explants for callus tissue induction were leaves，followed by petioles and finally root segments，with induction rates of 70%，40% and 35% respectively；the best medium for callus tissue induction was 1/2MS+6-BA0.5+2,4-D0.5；the best medium for callus tissue succession and adventitious shoot differentiation was MS+6-BA1.0+2,4-D0.2.The best medium for the succession and adventitious shoot differentiation was MS+6-BA1.0+NAA0.2；the best medium for the rooting of clumped shoots was 1/2MS+NAA0.2；the annual seedling yield mathematical formula of one bottle seedlings for the model of direct shoot，shoot and root segment production was Y=mXn；the annual seedling yield mathematical formula of one bottle seedlings for the model of regenerating mass or callus tissue for shoot production was Y=ac(1-Xn)/(1-X).
参考文献/References:
［1］ZHANG H X，WANG G L，QIAO Y X，et al.Plant regeneration from root segments of Anthurium andraeanum and assessment of genetic fidelity of in vitro regenerates［J］.In Vitro Cellular & Developmental Biology-Plant，2021，57(6):954-964.［2］褚云霞，任丽，邓姗，等.基于层次分析法的红掌种质资源评价［J］.上海农业学报，2023，39(1):40-47.［3］乔永旭，张永平，陈超，等.简易设施盆栽红掌周年高效栽培技术［J］.北方园艺,2009(5)：150-151.［4］任泠睿，乔永旭.红掌实生苗根段病原菌的抑制及再生体系的建立［J］.花卉，2018(2):1-2.［5］ZHANG H X，WANG G L，QIAO Y X，et al.Effects of timentin and other β-lactam antibiotics on callus induction，shoot regeneration，and rooting in Anthurium andraeanum Linden ex Andre［J］.In Vitro Cellular & Developmental Biology-Plant，2017,53(3):219-225.［6］王桂兰，陈超，李朝霞，等.红掌气生根根段再生快繁体系的建立［J］.植物生理学通讯，2005，41(3):297-301.［7］周媛，杨品，毛静,等.杜鹃‘胭脂蜜’组织培养与快速繁殖体系［J］.华中师范大学学报（自然科学版），2022，56(6):993-999.［8］JAHAN M T，ISLAM M R，KHAN R，et al.In vitro clonal propagation of anthurium (Anthurium andraeanum L.) using callus culture［J］.Plant Tissue Culture and Biotechnology，2014，19(1):61-69.［9］韩伟，包英华，于白音.红掌(Anthurium andraeanum)细胞工程研究进展及其在育种上的应用［J］.分子植物育种，2020，18(10)：3311-3322.［10］许丁帆，刘艳军，边一迪,等.红掌松散型胚性愈伤组织的诱导［J］.天津农学院学报，2021，28(3):22-25,30.［11］易双双，冷青云，黄少华,等.红掌新品种‘13100-A3’愈伤组织诱导及再生体系的建立［J］.分子植物育种，2022，20(4):1273-1280.［12］陈超.红掌愈伤组织和再生团块的发育及四倍体诱导［D］.南京：南京农业大学，2011.［13］陈育青.红掌的叶片培养及快速繁殖技术研究［J］.江西农业学报，2006，18(4)：104-105.
相似文献/References:
[1]张玉杰,杨 忠,顾德峰.狗枣猕猴桃叶片离体培养的研究[J].北方园艺,2014,38(10):97.
ZHANG Yu-jie,YANG Zhong,GU De-feng.Study on in vitro Culture of Actinidia kolomikta Leaves[J].Northern Horticulture,2014,38(24):97.
[2]赵兴华,吴海红,杨佳明.百合远缘杂交未成熟子房的离体培养研究[J].北方园艺,2014,38(04):90.
ZHAO Xing-hua,WU Hai-hong,YANG Jia-ming.Study on Lily Distant Hybridization in vitroCulture of Immature Ovary[J].Northern Horticulture,2014,38(24):90.
[3]刘作梅,王兰英,宋祥兰,等.“南高丛”蓝莓离体培养及增殖技术的研究[J].北方园艺,2013,37(12):103.
LIU Zuo-mei,WANG Lan-ying,SONG Xiang-lan,et al.Study on in vitro Culture and Proliferation of Vaccinium corymbosum[J].Northern Horticulture,2013,37(24):103.
[4]闫海丽,王秀玲,邵保康.快宝天然海藻肥对红掌生长的影响[J].北方园艺,2013,37(12):177.
YAN Hai-li,WANG Xiu-ling,SHAO Bao-kang.Effects of Kelpak Seaweed Extration on Growth of Anthurium[J].Northern Horticulture,2013,37(24):177.
[5]韩德伟.麒麟紫葳一步成苗离体培养技术研究[J].北方园艺,2013,37(03):122.
HAN De-wei.Study on in vitro Regeneration of One-tep Seedling for Radermachera sinica[J].Northern Horticulture,2013,37(24):122.
[6]武鹏,方锋学,黄如葵,等.苦瓜离体再生体系建立的研究[J].北方园艺,2012,36(02):124.
WU Peng,FANG Feng-xue,HUANG Ru-kui,et al.Study on the Establishment of in vitro Regeneration System in Momordica charontia L.[J].Northern Horticulture,2012,36(24):124.
[7]王安石,王健,林明光.红掌组培苗工厂化生产污染控制技术[J].北方园艺,2013,37(08):58.
WANG An-shi,WANG Jian,LIN Ming-guang.The Techniques of Pollution Control in Factory Production of Anthurium andraeanum Plantlets[J].Northern Horticulture,2013,37(24):58.
[8]赵志莲,李海峰,张德全,等.滇龙胆种质资源筛选及离体培养的初步研究[J].北方园艺,2013,37(08):168.
ZHAO Zhi-lian,LI Hai-feng,ZHANG De-quan,et al.Preliminary Study on the Screening of Germplasm Resources and the in Vitro Propagation for Gentiana rigescens[J].Northern Horticulture,2013,37(24):168.
[9]关丽霞.矮紫杉一步成苗离体培养技术[J].北方园艺,2012,36(03):80.
GUAN Li-xia.In Vitro Regeneration of One-tep Seedling for Taxus cuspidate cv.Nana[J].Northern Horticulture,2012,36(24):80.
[10]李永丽,周洲,郑涛,等.卡那霉素对108杨不定芽诱导的影响[J].北方园艺,2012,36(03):119.
LI Yong-li,ZHOU Zhou,ZHEN Tao,et al.Effects of Kanamycin on Adventitious Buds Induction of Populus×euramericana cl.‘Guariento’[J].Northern Horticulture,2012,36(24):119.
备注/Memo
第一作者简介：张永平（1978-），女，博士，教授，现主要从事园林园艺植物栽培生理生化等研究工作。E-mail：zh-yongping@163.com.责任作者：乔永旭（1978-），男，博士，教授，现主要从事园林园艺植物资源开发与利用等研究工作。E-mail：qiaoyx123@163.com.基金项目：江苏省苏北科技专项资助项目（SZ-SQ2021042）；宿迁市科技计划现代农业资助项目（L202208）；宿迁学院学科建设资助项目（2021ZDJS04）；宿迁学院人才引进科研启动基金资助项目（校2022XQT004）。收稿日期：2023-05-14