ZHAO Xing-hua,WU Hai-hong,YANG Jia-ming.Study on Lily Distant Hybridization in vitroCulture of Immature Ovary[J].Northern Horticulture,2014,38(04):90-93.
百合远缘杂交未成熟子房的离体培养研究
- Title:
- Study on Lily Distant Hybridization in vitroCulture of Immature Ovary
- 文章编号:
- 1001-0009(2014)04-0090-04
- Keywords:
- lily; distant hybridization; ovary; in vitroculture
- 分类号:
- S 682.2
- 文献标志码:
- A
- 摘要:
- 以不同百合种间杂交获得的子房为外植体，根据子房的发育程度，采用子房切片、胚珠或者小胚进行离体培养，观察子房切片、胚珠和小胚的萌发和生长情况，以期筛选出适合小胚、胚珠和子房切片萌发和生长的培养基。结果表明：适合子房切片培养的培养基为MS+NAA 0～0.3 mg/L+蔗糖30 000 mg/L；适合胚珠培养的培养基为1/2 MS+BA 0～0.2 mg/L+蔗糖30 000 mg/L；适合小胚培养的培养基为1/2 MS+BA 0～0.2 mg/L+NAA 0～0.2 mg/L+蔗糖30 000 mg/L。
- Abstract:
- Taking different inter specific hybridization obtained lily ovary as explants，according to the degree of development of the ovary，the slice using ovary，ovules or small embryos were cultured in vitroto observe sliced ovary，ovules and small embryo germination and growth，and filter out suitable germination and growth medium for small embryo，ovule and ovary slices.The results showed that good ovary slice culture medium was MS+NAA 0~0.3 mg/L+sucrose 30 000 mg/L；suitable ovule culture medium was 1/2 MS+BA 0~0.2 mg/L+sucrose 30 000 mg/L；suitable for small embryo culture medium was 1/2 MS+BA 0~0.2 mg/L+NAA 0~0.2 mg/L+sucrose 30 000 mg/L.
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备注/Memo
第一作者简介：赵兴华（1973-），男，吉林镇赉人，硕士，副研究员，现主要从事百合育种与花卉栽培技术等工作。E-mail：zhaoxh@163.com.