GUAN Li-xia.In Vitro Regeneration of One-tep Seedling for Taxus cuspidate cv.Nana[J].Northern Horticulture,2012,36(03):80-81.
矮紫杉一步成苗离体培养技术
- Title:
- In Vitro Regeneration of One-tep Seedling for Taxus cuspidate cv.Nana
- 文章编号:
- 1001-0009(2012)03-0080-02
- 分类号:
- S 791.49
- 文献标志码:
- A
- 摘要:
- 以矮紫杉当年生绿枝嫩梢为外植体,进行了一步成苗离体培养技术的研究。结果表明:矮紫杉一步成苗离体培养的外植体材料选用1.5%NaClO处理效果最好,在基本培养基MS+琼脂6 g/L+蔗糖30 g/L+AC 0.5 g/L中添加IBA 1.0 mg/L一步成苗率最高。
- Abstract:
- Using the health and greenwood slightly tender of Taxus cuspidate cv.Nana as explants,the technique of one-step-seedlingformation from in vitro culture were studied.The results showed that one-step seedling formation of one-step-seedlingformation from in vitro culture of Taxus cuspidate cv.Nana’s explants selection of 1.5% NaClO material handling best,in the basic medium MS + agar 6 g/L + sucrose 30 g/L + AC 0.5 g/L added IBA 1.0 mg/L step into the highest rate of seedlings.
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备注/Memo
作者简介:关丽霞(1978-),女,辽宁抚顺人,本科,实验师,现主要从事花卉与树木的组织培养研究工作。E-mail:llgx126@163.com。