SUN Qiaoye,LI Xingguo,MA Congfei,et al.Extraction and Identification of Anthocyanin From Red Raspberry[J].Northern Horticulture,2019,43(10):113-122.[doi:10.11937/bfyy.20183047]
红树莓花色苷的提取与鉴定
- Title:
- Extraction and Identification of Anthocyanin From Red Raspberry
- 关键词:
- 红树莓; 花色苷; 优化; 高效液相色谱-质谱联用; 组成鉴定
- Keywords:
- red raspberry; anthocyanin; optimizationn; high performance liquid chromatography-mass spectrometry(HPLC-MS); structural identification
- 文献标志码:
- A
- 摘要:
- 以“秋福”红树莓果实为试材，对其花色苷提取条件进行优化，并采用高效液相色谱质谱联用分析鉴定其花色苷组成成分。结果表明：“秋福”红树莓花色苷最佳提取条件为1.5%的盐酸-甲醇溶液，料液比1∶30 g?mL-1，提取时间3 h，提取温度35 ℃，此时花色苷含量可达4.49 mg?g-1。高效液相色谱-质谱联用分析鉴定出“秋福”红树莓中花色苷共有6种组成成分，其中含量最多为花青素-3-葡萄糖苷，其次为花青素-3-槐糖苷和花青素-3-芸香糖苷，三者含量总和占花色苷总含量的93.03%。
- Abstract:
- This study take ‘Autumn Bliss’ red raspberry as test material.The extraction conditions of anthocyanin were optimized，and then the anthocyanin components were identified by high performance liquid chromatography-mass spectrometry.The results showed that 1.5% hydrochloric acid-methanol solution as extraction agent，solid-liquid ratio of 1∶30 g?mL-1,extraction time of 3 hours,extraction temperature of 35 ℃，the anthocyanin content was 4.49 mg?g-1.There were about six kinds of component of anthocyanin have identified by high performance liquid chromatography-mass spectrometry in raspberry fruits,the content of cyanidin-3-glucosin was the highest，secondly were cyanidin-3-sophoroside and cyanidin-3-rutinoside，the sum of the three components accounted for 93.03% of the total anthocyanin content.
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备注/Memo
第一作者简介：孙侨冶（1993-），女，硕士研究生，研究方向为果树采后生理。E-mail:1404377468@qq.com.责任作者：于泽源（1961-），男，博士，教授，现主要从事果树采后生理等研究工作。E-mail:yzy@neau.edu.cn.收稿日期：2018-12-17