ZHANG Shuangshuang,CHEN Xiaobin,LIN Hui,et al.Water Extract Components and Pilot Extraction Process of JUNCAO-cultivated Ganoderma lucidum Spent Mushroom Substrate[J].Northern Horticulture,2019,43(10):108-113.[doi:10.11937/bfyy.20183184]
菌草灵芝菌糟提取物成分及中试提取工艺
- Title:
- Water Extract Components and Pilot Extraction Process of JUNCAO-cultivated Ganoderma lucidum Spent Mushroom Substrate
- Keywords:
- JUNCAO; SMSG-WE; component; polysaccharides; pilot extraction process
- 文献标志码:
- A
- 摘要:
- 以菌草灵芝菌糟为试材，采用国家标准、农业行业标准等测定了提取物的粗多糖、三萜类物质、氨基酸等成分含量，并在实验室采用正交实验法对其粗多糖进行提取，研究了不同的料液比、提取温度、提取时间和提取次数对菌糟多糖得率的影响，获得最优化的提取条件，在此基础上优化了菌草灵芝菌糟提取物中试生产工艺。结果表明：菌草灵芝菌糟提取物中主要成分粗多糖含量为24.16%，三萜类物质含量为0.82%，氨基酸总量为6.50%；实验室菌草灵芝菌糟粗多糖的最佳提取条件为料液比1∶20 g?mL-1，提取温度100 ℃，提取时间4 h，提取次数3次，在最佳条件下，菌草灵芝多糖得率为4.45%；确定菌草灵芝菌糟提取物中试提取条件为提取次数3次，第1次料液比1∶10 g?mL-1，加热至100 ℃后保持2 h，第2次料液比1∶8 g?mL-1，加热至100 ℃后保持2 h；第3次料液比1∶6 g?mL-1，加热至100 ℃后保持1.5 h，平均提取得率为20.03%。
- Abstract:
- JUNCAO-cultivated Ganoderma lucidum spent mushroom substrate was used as test material.The content of crude polysaccharides，triterpenes and amino acids and other ingredients in the water extract of Ganoderma lucidum spent mushroom substrate (SMSG-WE) was determined by using national standards，agricultural industry standards，and the extraction process of crude polysaccharides from SMSG was optimized by orthogonal test in the laboratory，the effects of different solid-liquid ratio，extraction temperature，extraction time and times on the yield of polysaccharide from Ganoderma lucidum spent mushroom substrate were studied to obtain the optimal extraction conditions.On this basis，the pilot production process of SMSG-WE was optimized.The results showed that the contents of crude polysaccharides，triterpenes and amino acids were 24.16%，0.82% and 6.50%.In laboratory the optimum extraction condition of polysaccharides was solid-liquid ratio 1∶20 g?mL-1，extracting temperature 100 ℃，extraction time 4 hours，extraction times for 3 times，under the best conditions，the polysaccharide yield is 4.45%;pilot extraction conditions of SMSG-WE were determined as follows,extraction for 3 times，for the first time the solid-liquid ratio 1∶10 g?mL-1，heated to 100 ℃ for 2 hours，second time solid-liquid ratio 1∶8 g?mL-1，heated to 100 ℃ for 2 hours，third time solid-liquid ratio 1∶6 g?mL-1，keep 1.5 hours after heated to 100 ℃，the average obtained at a rate of 20.03%.
参考文献/References:
[1]林占熺.菌草学[M].3版.北京:国家行政学院出版社,2013.[2]吴振忠,吴潇.菌草菌糠的研究开发和利用[J].食用菌,2010(2):4-6.[3]李济宸.菌糠饲料生产及使用技术[M].北京:金盾出版社,2006.[4]赖春芬,杨羽茜,张海洋,等.5种食用菌废菌糠的营养成分及胞外酶活性[J].贵州农业科学,2017,45(12):82-85.[5]潘军,廉红霞,高腾云,等.菌糠的营养价值及其在饲料中的应用[J].饲料资源,2009(21):38-41.[6]张旭辉,赵瑞华,李丹阳,等.超声法提取灵芝菌糠中总黄酮的工艺研究[J].延安大学学报(自然科学版),2017,36(12):63-66.[7]LIN Z B.Modern research on Ganoderma (Lingzhi)[M].4th ed.Beijing:Peking University Medical Press，2015.[8]LIU Y L,MEI L,LI J,et al.Effect of JUNCAO-cultivated Ganoderma lucidum spent mushroom substrate-hot water extract on immune function in mice[J].Tropical Journal of Pharmaceutical Research,2018,17(2):261-267.[9]刘艳玲,李卫东,林冬梅,等.菌草灵芝菌糟提取物对免疫力低下小鼠抗氧化能力的影响[J].福建农林大学学报(自然科学版),2015,44(3):303-307.[10]陈晓斌,张双双,林春梅,等.菌草菌糟作为饲料在奶牛饲养中的应用效果[J].福建农林大学学报(自然科学版),2013,42(2):179-183.[11]张双双,陈晓斌,林应兴,等.菌草灵芝菌糟对奶牛生产性能和血液生化指标的影响[J].福建农林大学学报(自然科学版),2014,43(1):76-79.[12]林占熺,高怀宾,林辉.菌草灵芝、菌糠和中草药饲料添加剂防治仔猪肠炎的效果[J].福建农林大学学报(自然科学版),2004,33(1):85-88.[13]李伟文.菌草鹿角灵芝多糖含量研究及其菌糠多糖应用初探[D].福州:福建农林大学,2010.[14]林占熺,张双双,陈晓斌,等.菌草灵芝菌糟水提物对奶牛生产性能和血液生化指标的影响[J].福建农林大学学报(自然科学版),2013,42(5):523-526.[15]广东省质量技术监督局.DB44/T 496-2008灵芝中三萜类物质的测定[S].广州:广东科技出版社,2008.[16]中华人民共和国农业部.NY/T 1676-2008食用菌中粗多糖含量的测定[S].北京:中国农业出版社,2008.[17]国家技术监督局.GB/T 6432-94饲料中蛋白质测定方法[S].北京:中国标准出版社,1994.[18]张丽英.饲料分析与饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.[19]国家质量监督检验检疫总局.GB/T 6434-2006饲料中粗纤维的含量测定 过滤法[S].北京:中国标准出版社,2006.[20]国家质量技术监督局.GB/T 18246-2000饲料中氨基酸的测定[S].北京:中国标准出版社,2000.[21]国家质量监督检验检疫总局.GB/T 13080-2004 饲料中铅的测定 原子吸收光谱法[S].北京:中国标准出版社,2004.[22]国家技术监督局.GB/T 13082-1991饲料中镉的测定方法[S].北京:中国标准出版社,1991.[23]国家质量监督检验检疫总局.GB/T 13081-2006饲料中汞的测定[S].北京:中国标准出版社,2006.[24]国家质量监督检验检疫总局.GB/T 13079-2006 饲料中总砷的测定[S].北京:中国标准出版社,2006.[25]中华人民共和国卫生部.GB/T 5009.23-2006食品中黄曲霉毒素B1、B2、G1、G2的测定[S].北京:中国标准出版社,2006.[26]JIANG J,SLIVOVA V,VALACHOVICOVA T,et al.Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3[J].International Journal of Oncology,2004,24(5):1093-1099.[27]伍松陵,李爱科,郝淑红.功能性多糖作为饲料添加剂的功效及应用前景展望[A].中国畜牧兽医学会动物微生态学分会.第三届第八次全国学术研讨会暨动物微生态企业发展战略论坛论文集[C].广东广州,2006.[28]杨美璐,李宇敏,张英,等.灵芝多糖的生物活性及其在动物生产中的应用研究[J].饲料研究,2018(3):55-58.[29]陈乃东,陈琼,何健.正交实验优选灵芝多糖的提取工艺[J].安徽农业科学,2013,41(19):8146-8148.[30]张树庭.提高灵芝提取物纯度与得率的重要意义[J].中国食用菌,2007,26(2):14.
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备注/Memo
第一作者简介：张双双（1986-），女，硕士，研究实习员，研究方向为菌草技术。E-mail：leaf136_q@163.com.责任作者：林占熺(1943-)，男，研究员，博士生导师，现主要从事菌草技术研究与应用及菌草产业发展的教学与科研等工作。E-mail：lzxjuncao@163.com.基金项目：福建农林大学科技创新专项基金资助项目（CXZX2017559）；福建省菌草生态产业协同创新中心资助项目(K80ND8002)。收稿日期：2019-01-15