[1]张宝智,胡永红,韩继刚,等.七个江南牡丹品种花瓣中类黄酮物质分析[J].北方园艺,2013,37(02):61-65.
　ZHANG Bao-zhi,HU Yong-hong,HAN Ji-gang,et al.Study on Flavonoids in Petals of Seven Jiangnan Tree Peony Cultivars[J].Northern Horticulture,2013,37(02):61-65.
点击复制

七个江南牡丹品种花瓣中类黄酮物质分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年02期 页码: 61-65 栏目: 出版日期: 2013-02-28
Title:
Study on Flavonoids in Petals of Seven Jiangnan Tree Peony Cultivars
文章编号:
1001-0009（2013）02-0061-05
作者:
张宝智1; 胡永红2; 3; 韩继刚2; 3; 刘群录1; 3; 4; 5
1.上海交通大学 农业与生物学院，上海 200240；
2.上海植物园，上海200231；
3.上海城市植物资源开发与应用工程技术研究中心，上海 200231；
4.农业部都市农业（南方）重点开放实验室，上海 200240；
5.上海交通大学 陆伯勋食品安全研究中心，上海 200240.
Author(s):
ZHANG Bao-zhi1; HU Yong-hong2; 3; HAN Ji-gang2; 3; LIU Qun-lu1; 3; 4; 5
1.School of Agriculture and Biology，Shanghai Jiaotong University，Shanghai 200240；
2.Shanghai Botanical Garden，Shanghai 200231；
3.Shanghai Engineering Research Center of Sustainable Plant Innovation，Shanghai 200231；
4.Key Laboratory of Urban Agriculture(South)，Ministry of Agriculture，Shanghai 200240；
5.Bor.S.Luh Food Safety Research Center，Shanghai Jiaotong University，Shanghai 200240
关键词:
江南牡丹; 花色苷; 黄酮; 黄酮醇
Keywords:
Jiangnan tree peony; anthocyanins; flavones; flavonols
分类号:
S 687.3
文献标志码:
A
摘要:
以“凤尾”、“凤丹白”、“西施”、“粉莲”、“昌红”、“呼红”和“云芳”7个江南牡丹品种为试材，利用UPLC-PDA和UPLC-Q-TOF MS技术对盛花期花瓣中类黄酮组分进行了定性和定量分析。结果表明：江南牡丹花瓣中共检测到15种类黄酮组分，其中花色苷4种：矢车菊素-3,5-二葡萄糖苷、矢车菊素-3-葡萄糖苷、芍药花素-3,5-二葡萄糖苷、芍药花素-3-葡萄糖苷；黄酮7种：木犀草素单糖苷（六碳糖）、木犀草素二糖苷（甲基五碳糖+六碳糖）、芹黄素单糖苷（六碳糖）、芹黄素二糖苷（五碳糖+六碳糖）、芹黄素二糖苷（甲基五碳糖+六碳糖）、金圣草黄素单糖苷（六碳糖）、金圣草黄素二糖苷（六碳糖+甲基五碳糖）；黄酮醇4种：槲皮素单糖苷（六碳糖）、槲皮素二糖苷（六碳糖+六碳糖）、山奈酚二糖苷（六碳糖+六碳糖）、异鼠李素二糖苷（六碳糖+六碳糖）；江南牡丹花瓣中主要的花色苷为芍药花素-3,5-二葡萄糖苷和矢车菊素-3,5-二葡萄糖苷，主要的黄酮为芹黄素糖苷，即芹黄素单糖苷（六碳糖）、芹黄素二糖苷（五碳糖+六碳糖）、芹黄素二糖苷（甲基五碳糖+六碳糖），黄酮醇为山奈酚糖苷，即山奈酚二糖苷（六碳糖+六碳糖）。
Abstract:
Utilizing 7 traditional cultivars of Jiangnan tree peony，‘Fengwei’，‘Fengdanbai’，‘Xishi’，‘Fenlian’，‘Changhong’，‘Huhong’，‘Yunfang’ as materials，the flavonoids in the petals at full-bloom stage were analyzed and quantified through UPLC-PDA and UPLC-Q-TOF MS.The results showed the existence of 15 flavonoids in the petals，including 4 anthocyanins：peonidin 3，5-di-O-glucoside (Pn3G5G)，cyanidin 3，5-di-O-glucoside (Cy3G5G)， cyaniding 3-O- glucoside (Cy3G)，peonidin 3-O-glucoside (Pn3G)，seven flavones：luteolin hexoside，luteolin deoxyhexo-hexoside，apigenin-hexoside，apigenin pento-hexoside，apigenin deoxyheso-hexoside，chrysoeriol hexoside，chrysoeriol deoxyhexo-hexoside and four flavonols：quercetin hexoside，quercetin di-hexoside，kaempferol di-hexoside，isorhamnetin di-hexoside.The main anthocyanins in the petals of Jiangnan tree peony were Pn3G5G and Cy3G5G，and the main flavones/flavonols were apigenin-hexoside，apigenin pento-hexoside，apigenin deoxyheso-hexoside and kaempferol di-hexoside respectively.

参考文献/References:

[1]王莲英.中国牡丹品种图志[M].北京:中国林业出版社,1997.

[2]Wang L S,Hashimoto F，Shiraishi A，et al.Chemical taxonomy of the Xibei tree peony from China by floral pigmentation [J].Journal of Plant Research,2004,117(1):47-55.

[3]Wang L S,Hashimoto F,Shiraishi A,et al.Phenetics in tree peony species from China by flower pigment cluster analysis [J].Journal of Plant Research,2001,114(3):213-221.

[4]Wang L S,Shiraishi A,Hashimoto F,et al.Analysis of petal anthocyanins to investigate flower coloration of Zhongyuan (Chinese) and Daikon Island (Japanese) tree peony cultivars [J].Journal of Plant Research,2001,114(1):33-43.

[5]Fan J L,Zhu W X,Kang H B,et al.Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree penoy cultivars [J].Journal of Functional Foods,2012,4(1):147-157.

[6]Li C,Du H,Wang L,et al.Flavonoid composition and antioxidant activity of tree peony (Paeonia?Section Moutan) yellow flowers [J].Journal of Agricultural and Food Chemistry,2009,57(18):8496-8503.

[7]Wang X,Cheng C,Sun Q,et al.Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa?by high-speed counter-current chromatography [J].Journal of Chromatography,2005,1075(1):127-131.

[8]樊金玲,朱文学,沈军卫,等.高效液相色谱-电喷雾质谱法分析牡丹花中花色苷类化合物[J].食品科学,2007,28(8):367-371.

[9]樊金玲,朱文学,沈军卫,等.红色系中原牡丹品种花色苷和黄酮的含量分析[J].北方园艺,2009(10):191-194.

[10]Heursel J.Diversity of flower colours in?Rhododendron simsii Planch and prospects for breeding [J].Euphytica,1981,30(1):9-14.

[11]Katsumoto Y,Masako F M,Yuko F,et al.Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin [J].Plant and Cell Physiology,2007,48(11):1589-1600.

[12]De V N,Jeroen T H,Henk-Peter V S,et al.A cytochrome b5 is required for full activity of flavonoid 3′,5′-hydroxylase，a cytochrome P450 involved in the formation of blue flower colors[J].Proceedings of the National Academy of Sciences,1999,96(2):778-783.


相似文献/References:

[1]王哲,徐皓.不同产地紫山药中花色苷含量分析[J].北方园艺,2013,37(03):49.
　WANG Zhe,XU Hao.Analysis of Anthocyanins Content in Dioscorea alata L.from Different Producing Areas[J].Northern Horticulture,2013,37(02):49.
[2]常仁杰,何 勇,朱祝军.植物花色苷抗逆性作用研究进展[J].北方园艺,2013,37(09):216.
　CHANG Ren-jie,HE Yong,ZHU Zhu-jun.Research Progress of the Resistance of Plant Anthocyanin to Stress[J].Northern Horticulture,2013,37(02):216.
[3]张俊环,杨 丽,孙浩元,等.不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J].北方园艺,2012,36(24):1.
　ZHANG Jun-huan,YANG Li,SUN Hao-yuan,et al.Changes of the Total Phenols and Flavonoids in Apricot Peel and Pulp of Different Cultivars During Fruit Development[J].Northern Horticulture,2012,36(02):1.
[4]尤扬,刘香坤.紫叶碧桃花色苷提取及其稳定性研究[J].北方园艺,2012,36(07):67.
　YOU Yang,LIU Xiang-kun.Study on the Extraction and Stability of Prunus persica Batsch.[J].Northern Horticulture,2012,36(02):67.
[5]肖凤艳.山葡萄花色苷稳定性研究[J].北方园艺,2013,37(16):33.
　XIAOFeng-yan.StudyonStabilityofAnthocyaninsfromVitisamurensis[J].Northern Horticulture,2013,37(02):33.
[6]李小龙,张振文.不同酿酒葡萄品种果实成熟过程中花色苷含量变化[J].北方园艺,2014,38(22):13.
　LI Xiao-long,ZHANG Zhen-wen.Change of Anthocyanins in Different Vitis vinifera Varieties During Ripening[J].Northern Horticulture,2014,38(02):13.
[7]张敏,刘刚,张雁南,等.响应面法优化蓝靛果花色苷提取工艺研究[J].北方园艺,2014,38(23):113.
　ZHANG Min,LIU Gang,ZHANG Yan-nan,et al.Optimization of Extraction of Lonicera edulis Anthocyanin by Response Surface Methodology[J].Northern Horticulture,2014,38(02):113.
[8]黄慧英,武国星,郑立军,等.正交实验优化紫叶甘蓝型油菜花色苷提取工艺[J].北方园艺,2015,39(21):125.[doi:10.11937/bfyy.201521032]
　HUANG Huiying,WU Guoxing,ZHENG Lijun,et al.Optimization Technology of Extracting Anthocyanins From the Purple Leaf Brassica napus by Orthogonal Experiment[J].Northern Horticulture,2015,39(02):125.[doi:10.11937/bfyy.201521032]
[9]王琴,王建友,李勇,等.我国黑果枸杞研究进展[J].北方园艺,2016,40(05):194.[doi:10.11937/bfyy.201605051]
　WANG Qin,WANG Jianyou,LI Yong,et al.Research Progress on Lycium ruthenicum Murr.in China[J].Northern Horticulture,2016,40(02):194.[doi:10.11937/bfyy.201605051]
[10]谢国芳,王瑞,周笑犁,等.蓝莓花色苷稳定性研究进展[J].北方园艺,2016,40(07):190.[doi:10.11937/bfyy.201607047]
　XIE Guofang,WANG Rui,ZHOU Xiaoli,et al.Research Progress on the Stability of Blueberry Anthocyanins[J].Northern Horticulture,2016,40(02):190.[doi:10.11937/bfyy.201607047]

备注/Memo

第一作者简介：张宝智（1987-），男，硕士，研究方向为园林植物与观赏园艺。E-mail：shllzbza@sjtu.edu.cn.

责任作者：刘群录（1970-），男，博士，副教授，硕士生导师，研究方向为园林植物育种。E-mail：liuql@sjtu.edu.cn。
基金项目：上海市重点科技攻关资助项目(10391901200;11391901101)。

更新日期/Last Update: 2014-08-30