[1]卫聪聪,江定,吴根堃,等.甘蓝ARF基因家族的鉴定与生物信息学分析[J].北方园艺,2017,41(19):16-24.[doi:10.11937/bfyy.20170746]
　WEI Congcong,JIANG Ding,WU Genkun,et al.Genome-wide Identification and Bioinformatics Analysis of ARF Gene Family in Brassica oleracea[J].Northern Horticulture,2017,41(19):16-24.[doi:10.11937/bfyy.20170746]
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甘蓝ARF基因家族的鉴定与生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年19 页码: 16-24 栏目: 出版日期: 2017-10-15
Title:
Genome-wide Identification and Bioinformatics Analysis of ARF Gene Family in Brassica oleracea
作者:
卫聪聪1; 江定2; 吴根堃2; 陈长明2
1.黄河水利职业技术学院,河南 开封 475000；2.华南农业大学 园艺学院,广东 广州 510642
Author(s):
WEI Congcong1; JIANG Ding2; WU Genkun2; CHEN Changming2
1.Yellow River Conservancy Technical Institute,Kaifeng,Henan 475000;2.Horticulture College,South China Agriculture University,Guangzhou,Guangdong 510642
关键词:
甘蓝; ARF; 生物信息学
Keywords:
Brassica oleracea; auxin response factor; bioinformatics
DOI:
10.11937/bfyy.20170746
文献标志码:
A
摘要:
生长素响应因子(auxin response factor,ARF)基因家族是植物特有的转录因子家族，在调控植物生长发育过程中起重要作用。以公布的甘蓝基因组数据库为基础，采用生物信息学方法鉴定出29个ARF基因，命名为BoARFs,研究分析了其基因结构、染色体分布、蛋白质保守结构域、系统进化树及表达模式，以期为甘蓝ARF基因家族功能的深入研究提供参考依据。结果表明：BoARF家族基因结构相对复杂，含有2~14个外显子；在染色体上呈不均匀分布，除BoARF29基因未定位到染色体上外，其它基因均定位在不同的染色体上；所有BoARF蛋白均具有保守的B3结构域和Auxin_resp结构域，但只有21个BoARF蛋白包含1~2个AUX/IAA结构域；转录组数据表达模式分析表明，ARF基因具有不同的组织表达模式，部分基因表现出组织特异性。
Abstract:
Auxin response factor (ARF) gene family is the specific transcription factor in plant,and plays an important role in regulation of plant organ development.Based on the published genomic database of Brassica oleracea,29 ARF genes were identified by bioinformatics,named BoARFs.Their genetic structure,chromosome distribution,conservative domain structure of protein,phylogenetic tree and expression pattern of reference were analyzed for the further research of Brassica oleracea ARF gene family function to provide the reference basis.The results showed that the gene structure of BoARF family was relatively complex and contained 2-14 exons.Expect the BoARF29,all other genes were located in different chromosomes.All BoARF proteins had conserved B3 domain and Auxin_resp domain,while only 21 BoARF proteins contained 1 or 2 AUX/IAA domains.Transcriptome data analysis showed that ARF gene had different tissue expression patterns,and some genes showed tissue specificity.

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备注/Memo

第一作者简介：卫聪聪（1983-），女，硕士，讲师，研究方向为观赏园艺。E-mail：weicongcong@yrcti.edu.cn.

责任作者：陈长明（1984-），男，博士，副教授，现主要从事蔬菜遗传育种与生物技术教学与科研等工作。E-mail：cmchen@scau.edu.cn.
基金项目：广东省科技计划资助项目(2015A020209117)。收稿日期：2017-04-06

更新日期/Last Update: 2017-10-11