[1]崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响[J].北方园艺,2013,37(24):31-34.
　CUI Lu-lu,YU Hai-xia,LI Jing-fu,et al.Effect of PEG on Seed Vigor of Aging Cabbage[J].Northern Horticulture,2013,37(24):31-34.
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聚乙二醇对老化甘蓝种子活力的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年24期 页码: 31-34 栏目: 出版日期: 2013-12-30
Title:
Effect of PEG on Seed Vigor of Aging Cabbage
文章编号:
1001-0009(2013)24-0031-04
作者:
崔路路; 于海霞; 李景富; 许向阳
东北农业大学 园艺学院，黑龙江 哈尔滨 150030
Author(s):
CUI Lu-lu; YU Hai-xia; LI Jing-fu; XU Xiang-yang
College of Horticulture，Northeast Agricultural University，Harbin，Heilongjiang 150030
关键词:
甘蓝; PEG; 人工老化; 自然老化; 种子活力
Keywords:
cabbage; PEG; artificial aging; natural aging; seed vigor
分类号:
S 565.401
文献标志码:
B
摘要:
以“晚丰”甘蓝种子为试材，采用不同分子量、浓度、处理时间的PEG渗透剂分别对自然老化和人工老化种子进行组合引发处理，比较了不同PEG处理组合对老化甘蓝种子可溶性蛋白质含量、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性的影响。结果表明：PEG对老化种子有一定修复作用，可以提高种子活力；PEG分子量因素对自然老化种子的影响最大，浓度次之，处理时间最小；而经PEG渗透调节后老化种子萌发代谢过程有所改变，其中MDA含量比对照降低，可溶性蛋白质含量有所提高，抗氧化酶系统活性高于对照组；同时因老化条件的不同，自然老化与人工老化在生理生化方面存在差异，对PEG引发反应也有所区别，自然老化种子PEG引发效果较明显。
Abstract:
Taking ‘Wanfeng’ cabbage seeds as material，PEG penetrant combination treatment with different molecular weight，concentration，treatment time were adopted to natural aging and artificial aging of seeds，the effects of different combination of PEG treatment on soluble protein content，malondialdehyde (MDA) content，superoxide dismutase (SOD)，catalase (CAT) and peroxidase (POD) activity were compared.The results showed that PEG had some repair effect on aging seed，could improve seed vigor.PEG molecular had significant influence on natural aging seeds，followed by was the factors of PEG concentration，the minimum was PEG treatment time.Aging seed metabolic germination had changed after PEG-osmotic processes，including MDA content was lower than control，soluble protein content increased，antioxidant enzymes activity was higher than CK.At the same time due to aging conditions，there were difference on physiological and biochemical between the natural aging and artificial aging seeds，including the PEG induced reactions，natural aging seeds had the most obvious induced reactions after PEG triggered.

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备注/Memo

第一作者简介：崔路路（1987-），男，硕士研究生，研究方向为蔬菜遗传育种研究。Email:neaucui@163.com.

责任作者：许向阳(1969-)，男，博士，研究员，博士生导师，研究方向为蔬菜遗传育种及生物技术和蔬菜种质资源。E-mail：xxy709@126.com.
基金项目：国家现代农业产业技术体系专项资金资助项目（CARS-25）；黑龙江省杰出青年科学基金资助项目（JC201204）；哈尔滨市科技创新人才研究专项资金资助项目（2011RFXXN031）；东北农业大学创新团队基金资助项目；东北农业大学博士启动基金资助项目（2009RC09）。
收稿日期：2013-09-03

更新日期/Last Update: 2014-12-06