[1]张玮滢,于丽杰.转il-4基因甘蓝后代的遗传及表现分析[J].北方园艺,2012,36(08):133-137.
　ZHANG Wei-ying,YU Li-jie.Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea[J].Northern Horticulture,2012,36(08):133-137.
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转il-4基因甘蓝后代的遗传及表现分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年08期 页码: 133-137 栏目: 出版日期: 2012-08-16
Title:
Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea
文章编号:
1001-0009（2012）08-0133-05
作者:
张玮滢; 于丽杰
黑龙江省普通高等学校植物生物学重点实验室,哈尔滨师范大学 生命科学与技术学院，黑龙江 哈尔滨 150025
Author(s):
ZHANG Wei-ying; YU Li-jie
Higher Education Key Laboratory of Plant biology，College of Life Sciences and Technology，Harbin Normal University,Harbin,Heilongjiang 150025
关键词:
甘蓝; 白细胞介素-4; 分子检测; 田间分析
Keywords:
cabbage; interleukin-4; molecule detection; field survey
分类号:
S 635.603.6
文献标志码:
A
摘要:
利用农杆菌介导法将il-4基因转入甘蓝品种“中甘11”，用已转化得到的T3、T4代甘蓝品种为试验材料，通过PCR扩增、PCR-Southern杂交、蛋白质浓度测定和ELISA技术，对il-4基因转化甘蓝的后代进行了检测，并对il-4基因在T3、T4代甘蓝中的传递规律以及后代甘蓝植株的田间表现进行了调查。结果表明：T3、T4代转il-4基因甘蓝阳性植株数较少，甘蓝中il-4基因出现性状分离。同时，田间调查表明，相对于对照组（非转基因）植株，转il-4基因阳性植株的田间抗病性下降，植株高度降低，结实率降低，单株结籽数减少，生长势等农艺性状降低。
Abstract:
The il-4 gene was transformed into Brassica oleracea cv.‘Zhonggan 11’ by using Agrobacterium-mediated method.The obtained T3 and T4 generation materials were detected by using PCR amplification，PCR-southern blotting，protein concentration determination and ELISA.Meanwhile the genetic rule of il-4 gene and the phenotypes of progenies were investigated.The results showed that a few positive transgenic cabbage plants were detected in T3 and T4 generation.The genetic rule of il-4 gene in transgenic cabbages didn’t according with Mendel law.Meanwhile，the field survey showed that the disease resistance，height，seed setting rate，growth potential decreased comparing to the control.

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备注/Memo

第一作者简介：张玮滢（1986-），女，在读硕士，现主要从事植物分子生物学研究工作。E-mail：zhangweiying860411@163.com。

责任作者：于丽杰（1961-），女，博士，教授，现主要从事分子生物学研究工作。E-mail：yulijie1961@126.com。
基金项目：黑龙江省普通高等学校骨干教师创新能力资助项目；黑龙江省自然科学基金资助项目（C0014）；哈尔滨市学科后备带头人基金资助项目（0071007002）。
收稿日期：2011-12-23

更新日期/Last Update: 2014-08-31