[1]梁春辉,黄敏,李秀平,等.沙姜的组织培养与快繁技术[J].北方园艺,2017,41(19):11-15.[doi:10.11937/bfyy.20170859]
　LIANG Chunhui,HUANG Min,LI Xiuping,et al.Tissue Culture and Rapid Propagation of Kaempferia galangal L.[J].Northern Horticulture,2017,41(19):11-15.[doi:10.11937/bfyy.20170859]
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沙姜的组织培养与快繁技术

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年19 页码: 11-15 栏目: 出版日期: 2017-10-15
Title:
Tissue Culture and Rapid Propagation of Kaempferia galangal L.
作者:
梁春辉1; 黄敏1; 李秀平1; 董斌1; 闫晓东2
1.广东农工商职业技术学院 热作系，广东 广州 510507；2.广州出入境检验检疫局，广东 广州 510623
Author(s):
LIANG Chunhui1; HUANG Min1; LI Xiuping1; DONG Bin1; YAN Xiaodong2
1.Department of Tropical Crops,Guangdong Agriculture Industry Business Polytechnic College,Guangzhou,Guangdong 510507；2.Guangzhou Entry-Exit Inspectional and Quarantine Bureau,Guangzhou,Guangdong 510623
关键词:
沙姜; 不定芽; 组织培养
Keywords:
Kaempferia galangal L.; adventitious bud; tissue culture
DOI:
10.11937/bfyy.20170859
文献标志码:
A
摘要:
以沙姜芽为外植体，研究不同因子对沙姜消毒、增殖、生根的影响，以期建立沙姜组培快繁体系，并为其脱毒技术研究提供参考。结果表明：沙姜芽使用75%酒精60 s+0.1%升汞（HgCl2）8 min灭菌效果最好，污染率为13.33%，成活率达86.67%；不同激素组合对沙姜不定芽的继代增殖效果各异，以2.00 mg?L-16-BA+0.25 mg?L-1NAA的激素配比的增殖效果最好，增殖系数为6.30，沙姜组培苗最佳的生根培养基为1/2MS+NAA 0.50 mg?L-1，平均生根数为15.32条。
Abstract:
The bud of Kaempferia galangal were was used as the materials,to study the effect of different factors on the disinfection,multiplication,root induction,so as to establish in vitro culture system and provide reference for detoxification.The results showed that the best disinfection method of the bulb explants of Kaempferia galangal was 75% ethanol for 60 seconds,followed by 0.1% HgCl2 8 minutes,the contamination rate was 13.33%,and the survival rate was 86.67%.The effects of hormones on proliferation of adventitious bud were diffferent,the appropriate medium for proliferation was MS+6-BA 2.00 mg?L-1+NAA 0.25 mg?L-1 with the multiplication coefficient of 6.30.The best medium for root induction was 1/2MS+NAA 0.50 mg?L-1 and the number of roots per plantlets was 15.32．

参考文献/References:

[1]中华人民共和国药典委员会.中国药典(2005年版) [S].一部.北京:化学工业出版社,2005:21.

[2]RUJJANAWATE C,KANJANAPOTHI D,AMOMLERDPISON D,et al.Anti-gastric ulcer effect of Kaemperia parviflora[J].Journal of Ethnopharmacol,2005,102:120-122.
[3]STEVENSON P C,VEITCH C N,SIMMONDS M S J.Polyoxygenated cyclohexane derivatives and other constituents from Kaempferia rotunda L.[J].Phytochemistry,2007,68：1579-1586.
[4]薛颖,村上明,小清水弘一.沙姜中抗促癌有效成分的分离鉴定[J].中国中药杂志,2002,27(7):522.
[5]王锐,何嵋,周云,等.山奈不同溶剂提取物对DPPH自由基的清除作用[J].安徽农业科学,2010,38(29):16232-16233.
[6]何自福,虞皓,李志慧,等.沙姜瘟的发生及田间遮阴防治试验[J].广东农业科学,2005(5):44-45.
[7]王教义.姜脱毒组培技术研究[J].山东农业科学,1999(6):7-9.
[8]邓年方,潘百明.姜的组织培养研究进展[J].安徽农业科学,2009,37(26):12406-12407.[9]佘小漫,何自福.广东沙姜青枯病菌株遗传多样性研究[C].中国植物病理学会,2012年学术年会论文集,2012.
[10]何自福,佘小漫,虞皓,等.沙姜瘟病原菌鉴定[J].农业实验与产业化,2006(4):142-144.[11]文衍堂,郑小波.海南岛广藿香、沙姜青枯病病原菌鉴[J].热带作物学报,1984,5(2):113-117.
[12]高山林.脱毒生姜高产栽培技术[J].农业新技术,2005(1):14-15.
[13]易诚,张天晓,梁称福.黄姜根茎组培快繁技术研究[J].黑龙江农业科学,2008(1):11-13.
[14]黄菊辉.生姜种质资源的离体繁殖和保存[J].中国农业科学,1995,28(2):24-30.
[15]葛胜娟.生姜组培苗的培育及其生产应用[J].中国农学通报,2007,23(5):75-78.
[16]杭玲,黄卓忠,江文,等.生姜组织培养快繁技术研究与应用[J].江苏农业科学,2006(5):125-127.
[17]梁称福,易诚.黄姜组培快繁技术试验研究[J].湖南环境生物职业技术学院学报,2006,12(2):135-138.
[18]张玲,马林,李卫锋.生姜组织培养的快繁技术[J].山地农业生物学报,2003,22(2):173-175.
[19]邱运亮,段鹏慧,赵华.植物组培快繁及时[M].北京:化学工业出版社,2012:58-65.

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备注/Memo

第一作者简介：梁春辉（1985-），女，硕士，实验师，现主要从事植物组织培养的教学与研究等工作。E-mail：chhliang@gdaib.edu.cn.

责任作者：闫晓东（1987-），男，博士，农艺师，现主要从事出入境植物研究等工作。E-mail：yanm16@163.com.
基金项目：广东省高等职业教育作物生产技术品牌专业建设资助项目（粤教高函[2015]191号）；广东省高等职业教育园林技术品牌专业建设资助项目（粤教高函[2016]293号）；广东农工商职业技术学院科研资助项目（xyyb1402）。
收稿日期：2017-04-06

更新日期/Last Update: 2017-10-11