GAO Ying- ying,WANG Chao,ZHANG Zhi jie.Electron Microscopic Observation on Male Sterile CMSPM and Maintainer Line of Cabbage[J].Northern Horticulture,2014,38(02):15-19.
甘蓝CMS不育系和保持系的电镜观察
- Title:
- Electron Microscopic Observation on Male Sterile CMSPM and Maintainer Line of Cabbage
- 文章编号:
- 1001-0009(2014)02-0015-05
- Keywords:
- cabbage; CMS; electron microscopy; anther abortion
- 分类号:
- S 63.5
- 文献标志码:
- A
- 摘要:
- 为研究甘蓝CMS不育系材料PM的败育机理，以甘蓝不育系PM和保持系PF为试材，用透射电镜观察其小孢子发育的整个过程。结果表明：不育系在花粉母细胞时期形状不规则，严重影响了减数分裂的正常进行；在单核小孢子期，绒毡层细胞不解体且异常膨大，挤压小孢子使其变形，粘连在一起，并出现了严重的质壁分离现象，最后降解的小孢子和绒毡层细胞充斥着整个花粉囊，导致花药败育。
- Abstract:
- Using transmission electron microscopy techniques，the anther and microspore development were studied,in order to obtain differences between CMSPM and its maintainer line of?Brassica oleraceaL.var.capitata L..The results showed that pollen mother cells (PMC) of CMSPM was irregular.Thus meiosis of PMC was influenced seriously.During mononuclear stage，the tapetal cells did not degenerated and expand abnormally，pressing the microsporocyte made to be out of shape，and microspore appeared serious plasmolysis phenomenon.Finally degradation of microspore and tapetal cells was full of the whole pollen sac which caused the anther abortion.
参考文献/References:
[1]Hirose T，Fujimc Y.Studies on the hybrid seed production by using a new male sterile line in pepper(Capsicum annuum?L.)Ⅱ.On the process of pollen degeneration[J].Jap Soc Hort Sci,1981,50(1):66-70. [2]许忠民,张恩惠.甘蓝胞质雄性不育系CMS158小孢子发生的细胞学研究[J].西北农业学报,2012,21(3):118-121. [3]余凤群,傅廷栋.甘蓝型油菜几个雄性不育花药发育的细胞形态学研[J].武汉植物学研究,1990,8(3):209-216. [4]危文亮,王汉中,刘贵华.甘蓝型油菜细胞质雄性不育系NCa花药发育的细胞学观察[J].中国农业科学,2005,38(6):1232-1237. [5]王福青,王翠兰,宋再华.大白菜雄性不育系88-3花药和花粉发育的细胞形态学观察[J].西北植物学报,2001,22(3):570-574. [6]卞春松.几份不同遗传类型甘蓝雄性不育花药发育的细胞形态学研究[D].北京:中国农业科学院研究生院,1994. [7]耿三省,王志源,将健箴,等.辣椒雄性不育系小孢子发生的细胞学观察[J].园艺学报,1994,2(2):165-169. [8]Kaul M L H.Male sterility in higher plants[D].Berlin：Springer verlag,1988:211-256. [9]张德双,张凤兰.大白菜CMS96不育系和保持系电镜观察[J].华北农学报,2012,27(2):133-139. [10]Aarts M G，Hodge R，Kalantidis K，et al.The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes[J].The Plant Journal，1997,12(3):615-623. [11]Jin W,Horner H T,Palmer R G.Genetics and cytology of a new genic male-sterile soybean[Glycine max(L.)Morr.][J].Sex Plant Reprod,1997,10(1):13-21. [12]谢潮添,魏冬梅,田惠桥.高等植物雄性不育的细胞生物学研究进展[J].植物生理与分子生物学学报,2006,32(1):17-23. [13]Vizcay-Barrena G,Wilson Z A.Altered tapetal PCD and pollen wall development in the Arabidopsis ms1 mutant[J].Journal of Experimenta Botany,2006,57（11）:2709-2717. [14]赵会芳.新型大白菜Ogura细胞质雄性不育系RC7不育机理研究[D].杨凌:西北农林科技大学,2010. [15]罗鑫娟,刘旭昊,王新宇.绒毡层小体,一种在花药绒毡层细胞中新发现的含油细胞器[J].细胞生物学杂志,2006,28（1）:61-65.
相似文献/References:
[1]马志峰,张恩惠,王智民.角额壁蜂对网室制种甘蓝授粉效果的影响[J].北方园艺,2013,37(13):29.
MA Zhi-feng,ZHANG En-hui,WANG Zhi-min.Pollination Effects of Osmia cornifrons on Breeding Cabbage in Net Houses[J].Northern Horticulture,2013,37(02):29.
[2]高海娜,王朝阳,张恩慧.甘蓝胚状体诱导成苗因素研究[J].北方园艺,2014,38(01):97.
GAO Hai-na,WANG Zhao-yang,ZHANG En-hui.Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)[J].Northern Horticulture,2014,38(02):97.
[3]崔路路,于海霞,李景富,等.磁场处理对结球甘蓝种子活力及萌发进程的影响[J].北方园艺,2014,38(02):20.
CUI Lu- lu,YU Hai- xia,LI Jing- fu,et al.Effect of Magnetic Treatment on Seed Vigor and Seed Germination of Cabbage[J].Northern Horticulture,2014,38(02):20.
[4]金晓霞,于海凤,于丽杰.农杆菌介导的人白细胞介素-4基因转化甘蓝的研究[J].北方园艺,2013,37(15):113.
JIN Xiao-xia,YU Hai-feng,YU Li-jie.Study on Agrobacterium mediated Inter leukin4 Gene Transferred into Cabbage[J].Northern Horticulture,2013,37(02):113.
[5]郭国寿.甘蓝品种比较试验[J].北方园艺,2012,36(15):38.
GUO Guo-shou.Comparison Test of Cabbage Varieties[J].Northern Horticulture,2012,36(02):38.
[6]张玮滢,于丽杰.转il-4基因甘蓝后代的遗传及表现分析[J].北方园艺,2012,36(08):133.
ZHANG Wei-ying,YU Li-jie.Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea[J].Northern Horticulture,2012,36(02):133.
[7]崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响[J].北方园艺,2013,37(24):31.
CUI Lu-lu,YU Hai-xia,LI Jing-fu,et al.Effect of PEG on Seed Vigor of Aging Cabbage[J].Northern Horticulture,2013,37(02):31.
[8]范林林,高丽朴,左进华,等.不同包装材料对甘蓝的保鲜效果[J].北方园艺,2015,39(11):112.[doi:10.11937/bfyy.201511028]
FAN Lin-lin,GAO Li-pu,ZUO Jin-hua,et al.Effect of Different Packaging on Storage of Cabbage[J].Northern Horticulture,2015,39(02):112.[doi:10.11937/bfyy.201511028]
[9]蔡 霞,李桂莲,孟平红,等.大蒜间套菜用糯玉米、夏秋大白菜间套甘蓝一年两季四收栽培模式[J].北方园艺,2015,39(03):52.[doi:10.11937/bfyy.201503016]
[J].Northern Horticulture,2015,39(02):52.[doi:10.11937/bfyy.201503016]
[10]金玉忠,李志民,赵福顺,等.无公害农产品甘蓝生产技术规程[J].北方园艺,2015,39(05):58.[doi:10.11937/bfyy.201505020]
[J].Northern Horticulture,2015,39(02):58.[doi:10.11937/bfyy.201505020]
备注/Memo
第一作者简介：高营营（1987- ），女，硕士，研究方向为蔬菜育种。E-mail:qhyy1314@126.com.