GAO Hai-na,WANG Zhao-yang,ZHANG En-hui.Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)[J].Northern Horticulture,2014,38(01):97-101.
甘蓝胚状体诱导成苗因素研究
- Title:
- Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)
- 文章编号:
- 1001-0009（2014）01-0097-05
- Keywords:
- cabbage; microspore; cotyledon type embryoids; plant regeneration
- 分类号:
- S 635
- 文献标志码:
- A
- 摘要:
- 以3个优良甘蓝品种为试材，进行了游离小孢子培养和胚状体植株再生技术研究，以期探讨不同甘蓝材料的基因型、胚状体类型、不定芽大小、烯效唑对不定芽诱导和生根的影响。结果表明：甘蓝子叶型胚容易发育单芽，鱼雷型胚和球型胚容易形成丛生芽；且单芽率和丛生芽率在不同基因型之间存在差异。高度大于2 cm的不定芽转接到生根培养基上后生长速度先慢后快，生根数少且细长；小于2 cm的不定芽生长速度先快后慢，生根数多且粗壮。小孢子不定芽在添加0.50 mg/L烯效唑的MS+0.3 mg/L NAA生根培养基中生根率最高且根系生长健壮，移栽成活率高达95.6%。
- Abstract:
- Taking 3 superior cabbage varieties as test materials,isolated microspores culture and embryoid plant let regeneration technology were studied,the effects of different genotypes,different type of embryoids,different size of shoots,uniconazole on embryoids seedling induction were discussed.The results showed that cotyledon embryoids were easily form to single plants,and torpedo embryoids and ball-type embryoids were easily form to multiple shoots in all different genotypes.The rate of single plants and multiple shoots were different in different genotypes.The size of adventitious shoots was larger than 2 cm,the growth rate showed slow to fast after transferred to rooting medium,and the roots were fine and longer,The size of adventitious shoots shorter than 2 cm,the growth rate showed fast to slow after transferred to rooting medium,and the roots were healthy and strong.Add 0.50 mg/L uniconazole MS+0.3 mg/L NAA regenerated plantlets rooting medium up to 95.6% survival rate of regenerated shoots thick stems and roots.
参考文献/References:
[1] Fletcher R,Coventry,Kott L S.Double haploid technology for winter Brassica napus [D].Canada University of Guelph,1998：42. [2] 杨丽梅，方智远，刘玉梅,等.“十一五”我国甘蓝遗传育种研究进展[J].中国蔬菜，2011(2)：1-10. [3] Kuginuki Y,Yoshikawa H,Hirai M.Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapaL.ssp.pekinensis)[J].European Journal of Plant Pathology,1999：105(4):327-332 [4] Chuong P V,Beversdorf W D,Powell A D,et al.The use of haploid protoplast fusion to combine cytoplasmic atrazine resistance and cytoplasmic male sterility in Brassicanapus[J].Plant CelTissue and Organ Culture,1988,12(2):181-184 [5] Caredda S,Doncoeur C,Devaux P,et al.Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgareL.)[J].Sexual Plant Reproduction,2000,13(2)：95-104. [6] 马文卿,李青,刘燕.大花蕙兰试管苗增殖过程中的褐化研究[J].中国农学通报,2010,26(7):186-190. [7] 崔广荣,刘云兵,谷业理.琼脂浓度、pH值及接种苗大小对文心兰试管苗增殖的影响[J].生物学杂志,2004,21(6):29-31. [8] 王会.红实美草莓试管苗瓶外生根的研究[J].长江蔬菜,2008(22):44-47. [9] 马勇斌,张恩慧,李殿荣,等.利用甘蓝游离小孢子不定芽叶片再生DH植株技术研究[J].西北农林科技大学学报(自然科学版),2011(4):111-116. [10]许念芳.诱导甘蓝小孢子高出胚率和成苗技术研究[D].杨凌:西北农林科技大学，2010:23. [11]周祖富,艾素云,杨美纯.烯效唑对罗汉果试管苗生长的影响[J].福建果树,2005(1):13-15. [12]葛云侠,陈凤玉.S3307对番茄插枝生根的作用[J].植物生理学通讯,2001,37(1):15-17.
相似文献/References:
[1]马志峰,张恩惠,王智民.角额壁蜂对网室制种甘蓝授粉效果的影响[J].北方园艺,2013,37(13):29.
MA Zhi-feng,ZHANG En-hui,WANG Zhi-min.Pollination Effects of Osmia cornifrons on Breeding Cabbage in Net Houses[J].Northern Horticulture,2013,37(01):29.
[2]高营营,王 超,张智杰.甘蓝CMS不育系和保持系的电镜观察[J].北方园艺,2014,38(02):15.
GAO Ying- ying,WANG Chao,ZHANG Zhi jie.Electron Microscopic Observation on Male Sterile CMSPM and Maintainer Line of Cabbage[J].Northern Horticulture,2014,38(01):15.
[3]崔路路,于海霞,李景富,等.磁场处理对结球甘蓝种子活力及萌发进程的影响[J].北方园艺,2014,38(02):20.
CUI Lu- lu,YU Hai- xia,LI Jing- fu,et al.Effect of Magnetic Treatment on Seed Vigor and Seed Germination of Cabbage[J].Northern Horticulture,2014,38(01):20.
[4]金晓霞,于海凤,于丽杰.农杆菌介导的人白细胞介素-4基因转化甘蓝的研究[J].北方园艺,2013,37(15):113.
JIN Xiao-xia,YU Hai-feng,YU Li-jie.Study on Agrobacterium mediated Inter leukin4 Gene Transferred into Cabbage[J].Northern Horticulture,2013,37(01):113.
[5]郭国寿.甘蓝品种比较试验[J].北方园艺,2012,36(15):38.
GUO Guo-shou.Comparison Test of Cabbage Varieties[J].Northern Horticulture,2012,36(01):38.
[6]张玮滢,于丽杰.转il-4基因甘蓝后代的遗传及表现分析[J].北方园艺,2012,36(08):133.
ZHANG Wei-ying,YU Li-jie.Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea[J].Northern Horticulture,2012,36(01):133.
[7]崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响[J].北方园艺,2013,37(24):31.
CUI Lu-lu,YU Hai-xia,LI Jing-fu,et al.Effect of PEG on Seed Vigor of Aging Cabbage[J].Northern Horticulture,2013,37(01):31.
[8]范林林,高丽朴,左进华,等.不同包装材料对甘蓝的保鲜效果[J].北方园艺,2015,39(11):112.[doi:10.11937/bfyy.201511028]
FAN Lin-lin,GAO Li-pu,ZUO Jin-hua,et al.Effect of Different Packaging on Storage of Cabbage[J].Northern Horticulture,2015,39(01):112.[doi:10.11937/bfyy.201511028]
[9]蔡 霞,李桂莲,孟平红,等.大蒜间套菜用糯玉米、夏秋大白菜间套甘蓝一年两季四收栽培模式[J].北方园艺,2015,39(03):52.[doi:10.11937/bfyy.201503016]
[J].Northern Horticulture,2015,39(01):52.[doi:10.11937/bfyy.201503016]
[10]金玉忠,李志民,赵福顺,等.无公害农产品甘蓝生产技术规程[J].北方园艺,2015,39(05):58.[doi:10.11937/bfyy.201505020]
[J].Northern Horticulture,2015,39(01):58.[doi:10.11937/bfyy.201505020]
备注/Memo
第一作者简介：高海娜（1988-），女，硕士，现主要从事蔬菜育种及栽培等研究工作。E-mail：danbai76@163.com.