LUO Qing,ZHANG Bo,LI Yan-long,et al.Effect of Temperature and Dark Culture on Anther Embryoid Induction of Lycium barbarum[J].Northern Horticulture,2014,38(17):111-113.
温度及暗培养对枸杞花药胚状体诱导的影响
- Title:
- Effect of Temperature and Dark Culture on Anther Embryoid Induction of Lycium barbarum
- 文章编号:
- 1001-0009(2014)17-0111-03
- Keywords:
- wolfberry anther; in vitro culture; embryoid; induction rate
- 分类号:
- R33
- 文献标志码:
- A
- 摘要:
- 以 “宁杞1号”花药为试材，进行离体培养诱导胚状体发生并获得完整植株，分别研究了低温预处理、高温热处理及暗培养对花药胚状体诱导的影响。结果表明：低温预处理5 d能显著提高胚状体诱导率，诱导率达1.43%；而33℃高温热处理和暗培养胚状体诱导率低于对照。
- Abstract:
- Taking ‘Ningqi No.1’ as test material,using method of anther embryoid induction to obtain regenerated plants.Some factors such as the pretreatment of low temperature,high temperature heat treatment,and dark culture were studied.The results showed that,the embryoid induction rate was significantly improved when the anther was treated 5 days under the the low temperature (4℃),and inducing rate was 1.43%.But embryoid induction rate was lower than the comparison by culture underh high temperature(33℃) and darkness.
参考文献/References:
［1］顾淑荣.枸杞花粉植株的获得［J］.植物学报,1981,23(3):246-248.
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备注/Memo
第一作者简介：罗青（1964-），女，副研究员，现主要从事枸杞遗传育种等研究工作。E-mail:luoqing640603@163.com.