SHI Sheng-feng,WANG Yong-zhi,LI Qi-yun,et al.Estalishment of Tissue Culture and Rapid Propagation of Commelina communis L.[J].Northern Horticulture,2012,36(24):111-114.
鸭跖草组织培养再生体系建立的研究
- Title:
- Estalishment of Tissue Culture and Rapid Propagation of Commelina communis L.
- 文章编号:
- 1001-0009（2012）24-0111-04
- 分类号:
- S 681.9
- 文献标志码:
- A
- 摘要:
- 以鸭跖草为试材，以无菌带节茎段、节间茎段、叶片为外植体，采用正交实验设计，研究了不同激素水平对鸭跖草再生体系的影响，以期探讨快速获得大量无性系苗的有效方法。结果表明：最佳外植体为幼嫩的带节茎段；最佳诱导培养基配方为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L；最佳增殖培养基配方为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L；最佳生根培养基配方为1/2MS+IBA 0.2 mg/L+NAA 0.1 mg/L。
- Abstract:
- Taking Commelina communis L as material，using the stem segments with node，internode stems and leaves as explants，the effect of different concentrations of hormone on the regeneration system of Commelina communis L were studied by orthogonal experiment，in order to get a large number of clonal seedlings rapidly.The results showed that the best explant was tender stem segments with node；the best compounding for explant growth was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L；the best compounding for proliferation growth was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L；and the best compounding for root growth was 1/2MS+IBA 0.2 mg/L +NAA 0.1 mg/L.
参考文献/References:
[1] 中国科学院中国植物志编辑委员会 . 中国植物志 [M].13 卷 .3 分册 . 北京 : 科学出版社 ,1997:127. [2]陈建华,刘承平.鸭跖草的家庭盆栽莳养[J].中国花卉盆景,1995,16(3):115-116. [3]〖JP〗Jing S R，Lin Y F，Wang T W，et al.Microcosm wetlands for wastewater treatment with different hydraulic loading rates and macrophyte [J].J Environ Qual,2002,31(2):690-696. [4]王桂云,方芳.鸭跖草属药用植物的研究进展[J].国际中医中药杂志,2008,30(6):474-476. [5]〖JP〗Zhang G B，Bing F H，Liu J，et al.Effect of total alkaloids from Commelina communisL.on lung damage by influenza virus infection[J].Microbiol Immunol,2010,54(12):754-757. [6]Bing F H，Liu J，Li Z，et al.Anti-influenza-virus activity of total alkaloids from Commelina communisL.[J].Arch Virol,2009,154(11):1837-1840. [7]Youn J Y，Park H Y，Cho K H.Anti-hyperglycemic activity of Commelina communisL.：inhibition of alpha-glucosidase[J].Diabetes Res Clin Pract,2004,66(1):149-155. [8]马成亮.鸭跖草的栽培[J].特种经济动植物,2005,32(5):36-39. [9]张孟仁.IBA和NAA处理菊花扦插生根试验[J].北方园艺,2008(9):130-131. [10]戴云新,张健,李敏,等.NAA和IBA对非洲菊组培苗生根的影响[J].安徽农业科学,2009,37(19):8845-8847. [11]Bonga J M,Durzan D J.树木组织培养[M].阙国宁,郭达初,译.北京:中国林业出版社,1988.
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备注/Memo
第一作者简介：时圣凤（1985-），女，山东单县人，在读硕士，研究方向为园林植物遗传育种与生物技术。E-mail：shishengfeng9@163.com.