GAO Jin-qiu,YU Li-jie,TAO Lei.Study on Transferred Interleukin-4 Gene in Daucus carota L. by Cell Transformation[J].Northern Horticulture,2012,36(24):115-119.
白细胞介素-4基因转化胡萝卜的研究
- Title:
- Study on Transferred Interleukin-4 Gene in Daucus carota L. by Cell Transformation
- 文章编号:
- 1001-0009（2012）24-0115-05
- 关键词:
- 胡萝卜; 白细胞介素-4基因（il-4基因）; 遗传转化; 根癌农杆菌
- Keywords:
- Daucus carota L.(carrot); interleukin-4 gene(il-4 gene); genetic transformation; Agrobacterium tumfaciens
- 分类号:
- Q 943.2；812
- 文献标志码:
- A
- 摘要:
- 以胡萝卜‘长城红玥’与‘开拓者’胚轴为受体材料，研究了菌种、预处理、农杆菌菌液浓度、侵染时间和共培养时间对胡萝卜胚轴转化率的影响。结果表明：C58C1菌株对胡萝卜胚轴伤害较小；‘长城红玥’以7日龄无菌苗于4℃预处理3 d，经3.2×107个/mL农杆菌菌液侵染6 min，共培养3 d，转化率可达60%；‘开拓者’以7日龄无菌苗于4℃预处理3 d，经4.8×107个/mL农杆菌菌液侵染10 min，共培养4 d，或7日龄无菌苗于4℃预处理10 d，经3.2×107个/mL农杆菌菌液侵染10 min，共培养2 d转化率可达100%；但2个品种均以7日龄无菌苗于4℃预处理3 d，经1.6×107个/mL农杆菌菌液侵染2 min，共培养2 d，可得最多转化苗。
- Abstract:
- Taking the hypocotyls of ‘Changchenghongyue’ and ‘Kaituozhe’ of Daucus carota L. as materials,the effect of the strains，the condition of pretreatment，concentration of Agrobacterium suspension，the time of infection and the time of co-cultivation on the transformation of carrot ‘Changchenghongyue’ and ‘Kaituozhe’ hypocotyls were studied.The results showed that the C58C1 strain was less damaged for carrot hypocotyls;and the transformation frequency was up to 60% for hypocotyls of ‘Changchenghongyue’，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 10 days，infected with suspension of A.tumefactions at the concentration of 3.2×107 cells/mL for 6 mins，co-cultivated for 3 days；and the transformation frequency was up to 100% for hypocotyls of ‘Kaituozhe’，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 3 days，infected with suspension of A.tumefactions at the concentration of 4.8×107 cells/mL for 10 min，co-cultivated for 4 days or derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 10 days，infected with suspension of A.tumefactions at the concentration of 3.2×107 cells/mL for 10 min，co-cultivated for 2 days;and the transformants were more for both of their hypocotyls，which derived from pretreatment 7-day-old aseptic plantlets at 4℃ for 3 days，infected with suspension of A.tumefactions at the concentration of 1.6×107 cells/mL for 2 mins，co-cultivated for 2 days.
参考文献/References:
[1] 朱晓艳 , 于丽杰 . 转基因胡萝卜的研究进展 [J]. 北方园艺 ,2012(2):188-190. [2]金伯泉,朱勇,李德敏,等.细胞和分子免役学[M].2版.北京:科学出版社,2001:139-141. [3]上海创未生物技术有限公司.重组人白细胞介素4介绍[EB/OL].http://www.biopro.net.cn/news/shownews.php?id=31.2010-03-03. [4]资讯频道.重组人白细胞介素-4[EB/OL].http://www.bio1000.com/news/cp/371988.html.2012-06-26. [5]Hardegger M,Sturm A.Transformation and regeneration of carrot (Daucus carotaL.)[J].Molecular Breeding,1998(4):119-127. [6]高金秋,于丽杰.胡萝卜体细胞胚再生系统的建立[J].北方园艺,2009(8):73-77. [7]王关林,方宏筠.植物基因工程原理与技术[M].2版.北京:科学出版社,2002:391,393,744. [8]王凌健,倪迪安,陈永宁,等.利用转基因胡萝卜表达肺结核疫苗[J].植物学报,2001,43(2):132-137. [9]Desfeux C，Clough S J，Bent A F.Female reproductive tissues are the primary target of Agrobact-erium-mediated transformation by the arabidopsis floraldip method[J].Plant Physiology,2000,123(3):895-904. [10]Ye G N,Stone D,Pang S Z,et al.Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation[J].The Plant Journal,1999,19(3):249-257. [12]郭蓉,张金文,刘玲,等.胡萝卜遗传转化体系的建立[J].甘肃农业大学学报,2005,40(4):535-539. [13]刘艳军,杨静慧,杨恩芹,等.提高农杆菌基因转化率方法的研究[J].西南农业大学学报,2003,25(2):144-146. [14]何泼,戴群,何放亭.泡桐遗传转化系统的建立[J].山东大学学报(自然科学版),1999,34(3):332-338. [15]王晓峰,鲁瑞芳,彭学贤,等.结球甘蓝外源基因转化再生时的激素条件研究[J].西北农业大学学报,2000,28(1):43-47. [16]黄学林,李筱菊.高等植物组织离体培养的形态建成及其调控[M].北京:科学出版社,1995:19.
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备注/Memo
第一作者简介：高金秋（1976-），女，硕士，讲师，研究方向为植物分子生物学,现主要从事基因工程及植物组织培养等的教学工作。E-mail:wawa760824@yahoo.com.cn.