LI Yu-wei,LIAN Rui-li,BAN Qiang,et al.Optimization of Liquid Fermentation Conditions for Ganoderma Lucidum Using Response Surface Methodology[J].Northern Horticulture,2012,36(13):179-182.
灵芝液体发酵培养条件的响应面法优化研究
- Title:
- Optimization of Liquid Fermentation Conditions for Ganoderma Lucidum Using Response Surface Methodology
- 文章编号:
- 1001-0009(2012)13-0179-04
- 分类号:
- S 567.3+1
- 文献标志码:
- A
- 摘要:
- 为获得更多的灵芝菌丝体产量，以单因素摇瓶试验法研究了发酵温度、发酵时间、发酵液的初始pH对灵芝液体深层发酵的影响；在此基础上，利用响应面法优化了灵芝液体发酵条件。结果表明：这3个因素对菌丝体产量的影响由大到小依次为发酵液的初始pH、发酵时间、发酵温度，而当发酵温度为28.7℃、发酵时间为124 h、发酵液的初始pH为5.9时，获得的菌丝体产量最大，为22.89 g/L，预测值与实测值吻合得较好。
- Abstract:
- For the yields of Ganoderma Lucidummycelium，different factors，including fermentation temperature，fermentation time，initial pH were studied comparatively by using single-factor test during the submerged fermentation of Ganoderma lucidum liquor.Based on the result of single-factor test，fermentation conditions were optimized by using response surface test.The results indicated that the three factors had different effects on the yields of Ganoderma Lucidummycelium and they were initial pH，fermentation time，fermentation temperature from high to low ordinarily；the maximum yields of Ganoderma Lucidummycelium (22.89 g/L) was acquired under the conditions that the temperature，fermentation time，initial pH were 28.7℃，124 h，5.9 respectively.
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备注/Memo
第一作者简介：李宇伟（1976-），男，河南汝州人，硕士，讲师，现主要从事高等真菌资源开发与药物制剂的教学与研究工作。
责任作者：李先芳（1964-），女，硕士，教授，现主要从事生物资源开发与应用方面的教学与研究工作。基金项目：河南省教育厅科技攻关计划资助项目（2011A230014）；河南省教育厅科技攻关计划资助项目（2009B210025）。
收稿日期：2012-03-10